Antitcoagulant and antiplatelet activities of scolymoside

Abstract

Cyclopia subternata is a medicinal plant commonly used in traditional medicine to relieve pain. Here, the anticoagulant effects of scolymoside, an active compound in C. subternata, were examined by monitoring activated partial thromboplastin time (aPTT), prothrombin time (PT), and the activities of thrombin and activated factor X (FXa). The effects of scolymoside on plasminogen activator inhibitor type 1 (PAI-1) and tissue-type plasminogen activator (t-PA) expression were evaluated in tumor necrosis factor (TNF)-α-activated human endothelial cells. Treatment with scolymoside resulted in prolonged aPTT and PT and the inhibition of thrombin and FXa activities and production. In addition, scolymoside inhibited thrombin-catalyzed fibrin polymerization and platelet aggregation. Scolymoside also elicited anticoagulant effects in mice, including a significant reduction in the PAI-1 to t-PA ratio. Collectively, these findings indicate that scolymoside possesses anticoagulant activities and could be developed as a novel anticoagulant. [BMB Reports 2015; 48(10): 577-582]

Inhibitory Effect of Zingerone on Secretory Group IIA Phospholipase A2

“…Cell Culture: Primary HUVECs were obtained from Cambrex Bio Science Inc. (Charles City, IA) and maintained as previously described [40][41][42].…”

“…Animals and Husbandry: Male C57BL/6 mice (6-7-weeks old, weighing 27 g) purchased from Orient Bio Co. (Sungnam, Republic of Korea), were maintained as described previously [40][41][42].…”

Suppressive effects of methylthiouracil on polyphosphate‐mediated vascular inflammatory responses

“…Primary human umbilical vein endothelial cells (HUVECs) were purchased from Cambrex Corporation (Charles City, IA, USA) and maintained as previously described . Briefly, the cells were cultured to confluency at 37°C and 5% CO 2 in endothelial basal medium (EBM)‐2 basal media supplemented with growth supplements (Cambrex Bio Science, Charles City, IA, USA).…”

Exploring the anticoagulant and antiplatelet effect of the extracts of the red marine alga Acanthophora spicifera

“…Thus, researchers are looking for new molecules with anticoagulant and/or antiplatelet effects able to improve therapies, but without restrictions or limitations. Lot of molecules derived from natural sources, be it from terrestrial or marine environment, have shown antihemostatic effects (Pimentel et al, 2003;de Andrade et al, 2011;Yoon et al, 2015;Lorigooini et al, 2015). Nonetheless, as known, most products with a clinical or medical interest came from oceans, approximately 33 and 25% from sponges and algae, respectively (Kijjoa and Sawangwong, 2004;Newman and Cragg, 2012).…”