Antisense-overexpression of the MsCOMT gene induces changes in lignin and total phenol contents in transgenic tobacco plants

Seong, Eun Soo; Yoo, Ji Hye; Lee, Jae Geun; Kim, Hee Young; Hwang, In Seong; Heo, Kweon; Kim, Jae Kwang; Lim, Jung Dae; Sacks, Erik J.; Yu, Chang Yeon

doi:10.1007/s11033-012-2255-y

Cited by 10 publications

(6 citation statements)

References 49 publications

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“…These results demonstrated that the efficiency of using an antisense approach with a heterologous gene, as shown previously using an endogenous homologous gene (Tottey et al 2003). The antisense expression of a heterologous gene results in reduced expression level of the endogenous homologous gene, which has been confirmed by the expression of antisense FBP1 (from Phaseolus vulgaris) in Arabidopsis (Bindschedler et al 2006), and antisense MsCOMT (from Miscanthus sinensis) and antisense GMPase (from tomato) in tobacco plants Seong et al 2013). Feedback inhibition of chlorophyll synthesis is observed.…”

Section: Discussionsupporting

confidence: 80%

Molecular characterization and primary functional analysis of PeMPEC, a magnesium-protoporphyrin IX monomethyl ester cyclase gene of bamboo (Phyllostachys edulis)

et al. 2015

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A homologous gene of MPEC from Phyllostachys edulis was isolated and characterized. Its overexpression in Arabidopsis thaliana increased chlorophyll concentration and photosynthesis efficiency, indicating it is involved in chlorophyll biosynthesis. Magnesium-protoporphyrin IX monomethyl ester cyclase (MPEC) is an essential enzyme in the biosynthesis of chlorophyll, which plays an important role in photosynthesis. However, limited information is available on the roles of MPEC gene in bamboo. A homologous gene, PeMPEC was identified from Phyllostachys edulis, which comprised 1474 bp and contained an open reading frame encoding 415 amino acids. PeMPEC was transcribed abundantly in leaf blade where photosynthesis predominantly occurs, which agreed with the protein accumulation pattern confirmed by Western blotting. The PeMPEC transcription was promoted by continuous darkness for 24 h, and was suppressed by increasing light intensity (100-1500 µmo1 m(-2) s(-1)) and high temperature (42 °C). However, transcription was induced within 0.5 h and thereafter declined with prolonged treatment (up to 12 h) under low temperature (4 °C). Although PeMPEC expressed weakly in etiolated leaves, transcript levels increased gradually with subsequent light treatment (200 µmol m(-2) s(-1)). Overexpression of PeMPEC in Arabidopsis thaliana resulted in increased chlorophyll concentration and photosynthesis efficiency in sense transgenic plants compared with a reduction in antisense transgenic plants. These changes were consistent with the transcript levels of PeMPEC. These results indicated that PeMPEC might be involved in chlorophyll biosynthesis and play important roles in maintaining the stability of photosystems, and provide a basis for the study of chlorophyll biosynthesis and dissection of photosynthesis in bamboo.

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Section: Discussionsupporting

confidence: 80%

Molecular characterization and primary functional analysis of PeMPEC, a magnesium-protoporphyrin IX monomethyl ester cyclase gene of bamboo (Phyllostachys edulis)

et al. 2015

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“…The silencing of HCT gene resulted in the decrease in lignin content and the change in lignin composition in the callus of Pinus radiata [55]. Antisense overexpression of the MsCOMT gene induces changes in lignin and total phenol contents in transgenic tobacco plants [56]. The transcription of lignin synthesis genes 4CL and CAD are upregulated, leading to lignin deposition, thickening of secondary cell walls, and enhancing of the salt-resistance and permeability of birch and apple [57,58].…”

Section: Discussionmentioning

confidence: 99%

Physiology, Transcriptome and Root Exudates Analysis of Response to Aluminum Stress in Pinus massoniana

Ling

Tan

Chen

et al. 2023

Forests

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Pinus massoniana is an important timber tree species in southern China, and acid aluminum stress seriously endangers its growth. This study focuses on physiology, gene regulation and root exudates. Aluminum stress increased the activity of malondialdehyde (MDA), proline (PRO), peroxidase (POD), soluble proteins (SP), soluble sugars (SS) and superoxide dismutase (SOD) in P. massoniana seedlings, and led to changes in growth. We identified hub genes (UCHL3, TCP1, SEC27, GluRS and ACTF) responding to aluminum stress of low concentration and hub genes (RGP, MPT, RPL24, RPL7A and EC3.2.1.58) responding to aluminum stress of high concentration. Aluminum stress mainly affected phenylpropanoid biosynthesis and flavonoid biosynthesis, and it may alleviate aluminum toxicity by inducing the upregulation of genes such as CHS, COMT, DFR and LAR to enhance root exudation of catechin. These results lay the foundation for in-depth studying the molecular mechanism of P. massoniana aluminum stress.

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“…The transgenic tobacco plants with down-regulated COMT gene expression demonstrate that COMT plays a crucial role related to controlling lignin and phenol content in plants. Moreover, COMT activity may be related to flavonoid production in the plant lignin pathway [ 47 ]. For this, studies have shown that plants strengthen their cell walls through the accumulation of phenolic compounds, which was thought to be a ubiquitous defense response [ 48 ].…”

Section: Discussionmentioning

confidence: 99%

RNA-Seq and Iso-Seq Reveal the Important Role of COMT and CCoAOMT Genes in Accumulation of Scopoletin in Noni (Morinda citrifolia)

Jia

Chen

Gong

et al. 2022

Genes

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Scopoletin, the main component of clinical drugs and the functional component of health products, is highly abundant in noni fruit (Morinda citrifolia). Multiple enzyme genes regulate scopoletin accumulation. In the present study, differentially expressed genes of noni were analyzed by RNA sequencing (RNA-Seq) and the full-length genes by isoform-sequencing (Iso-Seq) to find the critical genes in the scopoletin accumulation mechanism pathway. A total of 32,682 full-length nonchimeric reads (FLNC) were obtained, out of which 16,620 non-redundant transcripts were validated. Based on KEGG (Kyoto Encyclopedia of Genes and Genomes) annotation and differential expression analysis, two differentially expressed genes, caffeic acid 3-O-methyltransferase (COMT) and caffeoyl-CoA O-methyltransferase (CCoAOMT), were found in the scopoletin accumulation pathway of noni. Real-time quantitative polymerase chain reaction (q-PCR), phylogenetic tree analysis, gene expression analysis, and the change in scopoletin content confirmed that these two proteins are important in this pathway. Based on these results, the current study supposed that COMT and CCoAOMT play a significant role in the accumulation of scopoletin in noni fruit, and COMT (gene number: gene 7446, gene 8422, and gene 6794) and CCoAOMT (gene number: gene 12,084) were more significant. These results provide the importance of COMT and CCoAOMT and a basis for further understanding the accumulation mechanism of scopoletin in noni.

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Antisense-overexpression of the MsCOMT gene induces changes in lignin and total phenol contents in transgenic tobacco plants

Cited by 10 publications

References 49 publications

Molecular characterization and primary functional analysis of PeMPEC, a magnesium-protoporphyrin IX monomethyl ester cyclase gene of bamboo (Phyllostachys edulis)

Molecular characterization and primary functional analysis of PeMPEC, a magnesium-protoporphyrin IX monomethyl ester cyclase gene of bamboo (Phyllostachys edulis)

Physiology, Transcriptome and Root Exudates Analysis of Response to Aluminum Stress in Pinus massoniana

RNA-Seq and Iso-Seq Reveal the Important Role of COMT and CCoAOMT Genes in Accumulation of Scopoletin in Noni (Morinda citrifolia)

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