Antiradical properties of chemo drug, carboplatin, in cooperation with ZnO nanoparticles under UV irradiation in putative model of cancer cells

Antitumor activities of carboplatin–doxorubicin–ZnO complexes in different human cancer cell lines (breast, cervix uteri, colon, liver and oral) under UV exposition

“…The strongest efficiency of suppressing tumour growth through the abundant accumulation of this antitumor agent was caused because of the following reasons. One, the cell killing effect by native ZnO nanoparticles is not caused by the toxicity of ZnO, which conforms with the results reported in previous studies [21,22]. Two, DOX, as a cell proliferation and apoptotic marker, provides specific recognition fluorescence signals.…”

“…After homogenization of five different concentrations of chemo drug combinations as displayed in Table 2 (5 min of vortex for 30 min at 27 C), ZnO was blended in the mix of chemo drugs (5 min of vortex for 24 h at 27 C). The weight ratio of ZnO:(CP þ DOX) was preserved at 5:1, in accordance with a previous protocol [4,22]. The mix solution was centrifuged at 5000 rpm for 15 min to obtain the pellet-bound chemo drug compound.…”

“…For comparison of antioxidant activity of each chemo drug on DPPH Ã by the DPPH protocol [22,23], 1 mL of individual chemo drug solution was dispensed in a sterile test tube and sonicated with 2 mL of 0.1 nM methanolic DPPH Ã for 90 min. Six chemo drugs were tested without UV exposure for 24 h at 37 C, while other three were introduced into the 8 W, 400 nm UV chamber for 24 h at 37 C (Spectroline: CX-21, Kleinblittersdorf, Germany).…”

“…Figure 11 confirms the mean fluorescent intensity (MFI) with corresponding cellular apoptosis, which was obtained using flow cytometry analysis. A significant number of ZnO transported into the cells [21] emits intense intrinsic fluorescence caused by DOX to track the live cells [4], whereas CP enhances the response of oxidizers under UV exposition [22]. The cytoplasm of the live cells was therefore visualized in red.…”

“…Furthermore, the cellular cytotoxicity of ZnO nanoparticles depends on the concentration and reaction time. DOX offers an excellent fluorescence signal [4], whereas a combination of ZnO and CP with UV ray offers an enhanced response of oxidizers [22].…”