ANTIPROLIFERATIVE EFFECT OF Dendrophthoe pentandra EXTRACTS TOWARDS HUMAN BREAST ADENOCARCINOMA CELLS (MCF-7)

Abstract

Dendrophthoe pentandra (DP) is a semi-parasitic plant. Previous studies showed that this plant possessed diverse medicinal properties. The present study was carried out to determine antiproliferative activity of various DP crude extracts. Extracts of petroleum ether, diethyl ether, chloroform, ethyl acetate and methanol, have been evaluated through IC50 value, which defined by the concentration of drug that is required for the inhibition of 50% of cell population in vitro. The determination of percentage cell viability was conducted by using MTT assay. The screening assay involved the use of malignant cell line MCF-7 (breast cancer cell) whereas the normal cell line was L929 (connective cell). The control drug used was tamoxifen. The ethyl acetate and methanol extracts were found to be more effective against MCF-7 cancer cell lines compared to the others. All extracts showed no remarkable effect against normal cell L929 with IC50 values more than 100 µg/mL. In contrast to tamoxifen, all extracts showed less cytotoxicity effect towards normal cells. The data obtained from this study provide notable preliminary information on the cytotoxic/antiproliferative effect of different DP extracts and further study should be carried out to isolate the active compound of the most potential DP extracts.

Study of Dendrophthoe Pentandra Ethyl Acetate Extract as Potential Anticancer Candidate on Safety and Toxicity Aspects

“…Zainuddin et al [21] proposed that the traditional claimed on the medicinal plant DP might be contributing to the existence of these compounds in DP. The finding of the present research project was in line with the result from Zainuddin et al [21,28] where benzoic acid, hexadecanoic acid and linolenic acid were in common. Though the GC-MS analysis derived possibility of 39 compounds of DPEA, the purity of the sample would be concerned but the result obtained still considered as valid as bioactive compounds detected supported by previous research.…”

“…For this screening assay, malignant cell lines MCF-7 and L929 cells showed significant anti-proliferative activity. From the previous study, Zainuddin stated that different types of extract could reveal different therapeutic effects towards MCF-7 breast cancer cells [28]. The present finding in tandem of the previous work where DPEA inhibit MCF-7 cell growth in MTT treatment.…”

“…Saponin has the antioxidant effect [22], antiglycation [22,23], anti-inflammatory [24], surfactant activity due to amphipathic nature [25], complexation with cholesterol and causes hemolysis in erythrocyte on iv, enhance proteins penetration through membranes and augment the cytotoxicity of targeted immunotoxins directed against human cancer cells [26]. Researchers also in line with the present finding where DP had shown to have anticancer and free radical scavenging potency by means of anticancer activity on cell proliferation inhibition and inducing apoptosis of breast cancer cell lines T47D and MCF-7 [21,27,28].…”

“…Another study from Zainuddin [21,28] performed MBA assay and reported that no IC 50 detected in MDCK, L929 and Vero cell lines when tested with several concentrations of the extract. This indicates that the DP extract does not show any severe cytotoxic effect on mammalian normal cells from previous studies.…”

Study of Dendrophthoe Pentandra Ethyl Acetate Extract as Potential Anticancer Candidate on Safety and Toxicity Aspects

“…From the literature search, DP was found out to have antiproliferative activity on breast cancer cell line T47D cell proliferation and induce apoptosis using MTS assay [27]. Another study from Zainuddin [21,28] performed MBA assay and reported that no IC 50 detected in MDCK, L929 and Vero cell lines when tested with several concentrations of the extract. This indicates that the DP extract does not show any severe cytotoxic effect on mammalian normal cells from previous studies.…”