Antiproliferative Effect and Induction of Apoptosis by Inula viscosa L. and Retama monosperma L. Extracts in Human Cervical Cancer Cells

Merghoub, Nawel; Benbacer, Laïla; Btaouri, Hassan El; benhassou, H. Ait; Terryn, Christine; Attaleb, Mohammed; Madoulet, Claudie; Benjouad, Abdelaziz; Mzibri, Mohamed El; Morjani, Hamid; Amzazi, S.

doi:10.5772/30025

Cited by 37 publications

(38 citation statements)

References 67 publications

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“…We have previously reported that I. viscosa L. extracts exhibited inhibit proliferation and induce apoptosis in cervical cancer cells. We demonstrated that extract target telomere machinery in cancer HeLa and SiHa cells [Merghoub et al, 2009[Merghoub et al, , 2016Benbacer et al, 2012]. The aim of our study is to verify that tomentosin, which has been found as one of the main components in hexanic extract of I. viscosa L., might target the telomere machinery and induce apoptosis in cervical cancer cells.…”

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confidence: 83%

Tomentosin Induces Telomere Shortening and Caspase‐Dependant Apoptosis in Cervical Cancer Cells

Merghoub

Btaouri

Benbacer

et al. 2016

J of Cellular Biochemistry

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Tomentosin, a natural sesquiterpene lactone purified from of Inula viscosa L., was investigated for its anti-proliferative, telomere shortening, and apoptotic effects on human cervical cancer HeLa and SiHa cell lines. Tomentosin was found to inhibit the growth of SiHa and HeLa cell lines in dose and time-dependent manner (IC values of 7.10 ± 0.78 μM and 5.87 ± 0.36 μM, respectively after 96 h of treatment). As evidenced by TTAGGG telomere length assay, tomentosin target specifically the telomeric overhang lengthening. This was confirmed by the evaluation of the cytotoxic effects of tomentosin in the foetal fibroblast Wi38 and JW10 cells which were derived from Wi38 and express hTERT, the telomerase catalytic subunit. We found that JW10 cells are 4.7-fold more sensitive to tomentosin which argues for telomere as its specific target. Furthermore, we found that tomentosin mediate this cytotoxic effect by inducing apoptosis and cell cycle arrest at G2/M phase. Morphological features of treated cells, as evidenced by Hoechst 33324 staining, revealed that the cytotoxic effect was due to induction of apoptosis. This was accompanied by pro-caspase-3 cleavage, an increase in caspase-3 activity and a cleavage of poly (ADP-ribose) polymerase (PARP). Moreover, tomentosin induced a decrease in mitochondrial membrane potential (ΔΨm) and an increase in reactive oxygen species (ROS), accompanied by a decrease in Bcl-2 expression. This indicates that tomentosin-induced apoptosis may involve a mitochondria-mediated signaling pathway. This study provides the first evidence that tomentosin targets telomere machinery and induces apoptosis in cervical cancer cells. The molecular mechanism underlying tomentosin-induced apoptosis may involve a mitochondria-mediated signaling pathway. J. Cell. Biochem. 118: 1689-1698, 2017. © 2016 Wiley Periodicals, Inc.

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confidence: 83%

Tomentosin Induces Telomere Shortening and Caspase‐Dependant Apoptosis in Cervical Cancer Cells

Merghoub

Btaouri

Benbacer

et al. 2016

J of Cellular Biochemistry

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“…d'origine marocaine ont mis en évidence la présence de dix constituants : spartéine, N-méthylcytisine, 17-oxospartéine, lupanine, anagyrine [16,33], α-isospartéine, β-isospartéine, ammodendrine, cytisine et 5,6-déhydrolupanine [33]. Dans ce travail, seulement huit de ces alcaloïdes (tableau I) ont été retrouvés dans les feuilles de Retama monosperma (L.) Boiss.…”

Section: Discussionunclassified

“…L'anagyrine constitue seulement 18,93 % du contenu total en alcaloïdes contre 39,63 % selon Benbacer et coll. [16]. La lupanine et l'α-isospartéine n'ont pas été détectés dans nos échan-tillons.…”

Section: Discussionunclassified

“…Webb., qui pousse au Maroc dans les régions désertiques (Tafilalt) et le Moyen Atlas est essentiellement recommandée par les guérisseurs traditionnels [7,8] comme antidiabétique. En outre, il a été rapporté que ces espèces possèdent divers effets pharmacologiques associés entre autres à la présence des quinolizidines : effets hypoglycémique, diuré-tique [9][10][11], cytotoxique [12][13][14][15][16], antihypertenseur [17], antibactérien [18][19][20], antifongique, antiviral [18,21,22] et antioxydant [19,22].…”

Section: Introductionunclassified

“…Pour l'espèce Retama monosperma (L.) Boiss., originaire du Maroc, relativement peu d'études récentes ont été entreprises sur ses alcaloïdes [16,34,35]. Les résultats de ces recherches font apparaître des différences quantitatives notables, mais aussi des différences qualitatives essentiellement dues à la présence ou l'absence d'un alcaloïde ou d'un autre.…”

Section: Introductionunclassified

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Distribution des alcaloïdes dans les parties aériennes deRetama monosperma(L.) Boiss. du Maroc

et al. 2012

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Résumé -Introduction :Retama monosperma (L.) Boiss. fait partie de la famille des Fabaceae. C'est une espèce très utilisée en médecine populaire marocaine, certaines de ses propriétés biologiques et pharmacologiques ont été scientifiquement prouvées et sont probablement liées à la présence d'alcaloïdes. Matériels et méthodes : Les parties aériennes (tiges, feuilles, graines) d'un spécimen sauvage ont été récoltées dans la commune de Haouzia (El Jadida, Maroc). Les alcaloïdes ont été extraits de chaque partie de la plante par du méthanol puis analysés par chromatographie gazeuse couplée à la spectrométrie de masse. Résultats : Le contenu en alcaloïdes varie d'un organe à l'autre. Treize alcaloïdes connus ont été identifiés. La cytisine, la spartéine, l'ammodendrine et l'anagyrine sont les composés majoritaires. Le profil chimique des tiges est qualitativement très proche de celui des feuilles. Les graines ne montrent pas la même diversité en terme de structures chimiques. Conclusion : Les profils métaboliques trouvés pour les trois parties de la plante d'origine marocaine diffèrent largement de ceux reportés dans la littérature pour les espèces Retama. Mots clés :Retama monosperma (L.) Boiss., alcaloïdes, cytisine, spartéine, CPG-SM Abstract -Introduction: Retama monosperma (L.) Boiss. is a member of the family Fabaceae. This species is widely used in Moroccan folk medicine; some of its biological and pharmacological properties have been proven by several authors and are probably related to the presence of alkaloids. Materials and methods: The aerial parts (stems, leaves, seeds) of a wild specimen were collected in the municipality of Haouzia (El Jadida, Morocco). The alkaloids were extracted from each part of the plant with methanol and then analyzed by gas chromatography-mass spectrometry. Results and discussion: The alkaloid content varies from one organ to another. Thirteen known alkaloids were identified. Cytisine, sparteine, ammodendrine and anagyrine are the major compounds. The chemical profile of stems is qualitatively very similar to that of leaves. The seeds do not show the same diversity in terms of chemical structure. Conclusion: The metabolic profiles found for the three plant parts of Moroccan origin differ widely from those reported in the literature for the species Retama.

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Inula viscosa (L.) Aiton Ethanolic Extract Inhibits the Growth of Human AGS and A549 Cancer Cell Lines

Rechek

Haouat

Hamaidia

et al. 2023

Chemistry & Biodiversity

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The present study shows the chemical profile and cytotoxic properties of the ethanolic extracts of Inula viscosa from Northeast Algeria. The extract was obtained by maceration using ethanol. Its phenolic profile was determined using ultra-high-performance liquid chromatography coupled with a diode array detector and an electrospray mass spectrometer (UHPLC-DAD-ESI/MS), which allowed the identification and quantification of 17 compounds, 1,5-O-caffeoylquinic acid being the most abundant. The cytotoxic activity was assessed against human gastric cancer (AGS) and human non-small-cell lung cancer (A549) cell lines, whereas ethanolic extract elicited nearly 60 % and 40 % viability loss toward AGS and A549 cancer cells, respectively. Results also showed that cell death is caspase-independent and confirmed the involvement of RIPK1 and the necroptosis pathway in the toxicity induced by the I. viscosa extract. In addition, the ethanolic extract would not provoke morphological traits in the cancer cells. These findings suggest that I. viscosa can be a source of new antiproliferative drugs or used in preparation plant-derived pharmaceuticals.

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Antiproliferative Effect and Induction of Apoptosis by Inula viscosa L. and Retama monosperma L. Extracts in Human Cervical Cancer Cells

Cited by 37 publications

References 67 publications

Tomentosin Induces Telomere Shortening and Caspase‐Dependant Apoptosis in Cervical Cancer Cells

Tomentosin Induces Telomere Shortening and Caspase‐Dependant Apoptosis in Cervical Cancer Cells

Distribution des alcaloïdes dans les parties aériennes deRetama monosperma(L.) Boiss. du Maroc

Inula viscosa (L.) Aiton Ethanolic Extract Inhibits the Growth of Human AGS and A549 Cancer Cell Lines

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