Inula viscosa (L.) Aiton Ethanolic Extract Inhibits the Growth of Human AGS and A549 Cancer Cell Lines

Abstract

The present study shows the chemical profile and cytotoxic properties of the ethanolic extracts of Inula viscosa from Northeast Algeria. The extract was obtained by maceration using ethanol. Its phenolic profile was determined using ultra-high-performance liquid chromatography coupled with a diode array detector and an electrospray mass spectrometer (UHPLC-DAD-ESI/MS), which allowed the identification and quantification of 17 compounds, 1,5-O-caffeoylquinic acid being the most abundant. The cytotoxic activity was assessed against human gastric cancer (AGS) and human non-small-cell lung cancer (A549) cell lines, whereas ethanolic extract elicited nearly 60 % and 40 % viability loss toward AGS and A549 cancer cells, respectively. Results also showed that cell death is caspase-independent and confirmed the involvement of RIPK1 and the necroptosis pathway in the toxicity induced by the I. viscosa extract. In addition, the ethanolic extract would not provoke morphological traits in the cancer cells. These findings suggest that I. viscosa can be a source of new antiproliferative drugs or used in preparation plant-derived pharmaceuticals.

Phytochemical Profiling, Antimicrobial, Antiproliferative and Apoptotic Effects of Stemodia viscosa Roxb. of Western Ghats Region, India

“…These findings obtained in this study correlate with in vitro cytotoxicity results of Anula viscosa extract, a plant species belonging to the same genus as S. viscosa , the ethanolic extract of Anula viscosa was reported to have antiproliferative activity by inhibiting the proliferation of human gastric cancer cells (AGS) by 60 % and human lung cancer (A549) cells by 40 % [60] . The antiproliferative activity of S. viscosa observed in this study can be attributed to the identified compounds such as Phytol ( 7 ) with the GC/MS technique.…”

“…[76] B16F10 skin and COLO205 colon cancer cells were incubated at a concentration of 1x10 4 cells/mL in a culture medium at 37 °C with 5 % CO 2 for 24 h. The cells were seeded at a density of 104 cells/well in 100 μL of culture medium in microplates. Subsequently, the cells were exposed to various concentrations (20,40,60,80, and 100 μg/mL) of the 80 % ethanolic extract solution. Wells with DMSO (0.2 % in PBS) were used as controls, and the samples were incubated in triplicate.…”

Algerian Propolis from Distinct Geographical Locations: Chemical Profiles, Antioxidant Capacity, Cytotoxicity and Inhibition of 5‐Lipoxygenase Product Biosynthesis

“…[67] Caffeic acid is known to arrest cell cycle in a dose-and time-dependent manner and activate apoptosis, [68][69][70] while quercetin, a flavonoid present in propolis, inhibits the growth of human lung adenocarcinoma cell lines (A549 and HCC-44) [71] and JB6 P + cells [72] by inducing caspase-3-dependent apoptosis. [73][74][75] Ferulic acid was shown to modulate several molecular pathways involved in a multitude of biological functions. Accordingly, ferulic acid was shown to reverse multiple drug resistance against paclitaxel, [76] and regulated epithelial-to-mesenchymal transitions resulting in the inhibition of metastasis in MDA-MB-231 breast carcinoma cells.…”

Achiral Sm(III)-Based Metal–Organic Framework as a Luminescence Sensor for Enantiodiscrimination of Quinine and Quinidine

“…QN is the main active ingredient in drugs used to treat malaria, and it also has analgesic, antipyretic, and antiinflammatory properties. , In addition, QN is utilized as a flavoring agent and provides bitterness in soft drinks. , QD is the enantiomer of QN, which can also be employed in the treatment of malaria and arrhythmias in clinical applications. Excessive use of QN and QD can cause many side effects, such as nausea, headache, asthma, kidney failure, and nerve and cardiovascular damage. − Up to now, some methods for determining QN and QD are high-performance liquid chromatography, , chemiluminescence, , atomic absorption spectrometry, , and electrochemical analysis. , Nevertheless, these analytical methods are not very effective in distinguishing two enantiomers and have the disadvantages of high cost, complex preconditioning, and time consumption. Therefore, a quick, convenient, and economical detection method is necessary but challenging in the field of monitoring QN and QD.…”

“…Excessive use of QN and QD can cause many side effects, such as nausea, headache, asthma, kidney failure, and nerve and cardiovascular damage. 5−7 Up to now, some methods for determining QN and QD are highperformance liquid chromatography, 8,9 chemiluminescence, 10,11 atomic absorption spectrometry, 12,13 and electrochemical analysis. 14,15 Nevertheless, these analytical methods are not very effective in distinguishing two enantiomers and have the disadvantages of high cost, complex preconditioning, and time consumption.…”