Antiproliferative and cytotoxic effects of bioactive compounds isolated from Onosma bourgaei

Abstract

Onosma species have been used commonly for traditional medicine for years due to their bioactive compounds content. Onosma bourgaei aerial part was extracted with hexane and methanol successively. The methanol extract was subjected to chromatographic techniques to isolate allantoin (1), 3,4dihydroxybenzaldehyde (2), luteolin-7-O-glucoside (3), apigenin-7-O-β-glucoside (4), diosmetin-7-O-βglucoside (5), rosmarinic acid (6), globoidnan A (7). The structure of isolated compounds were identi ed by spectroscopic techniques such as 1D-NMR, 2D-NMR, FTIR, and LC-TOF/MS/MS. Antiproliferative activity of extract and natural compounds were carried out using HeLa (human epithelial cervix adenocarcinoma, ATCC® CCL-2™), HT-29 (human colorectal adenocarcinoma, ATCC® HTB38™), MCF-7 (human mammary gland adenocarcinoma, ATCC® HTB22™) and A549 (human lung carcinoma, ATCC® CCL185™) cancerous cells and normal cells, FL (human epithelial amnion cell, ATCC® CCL62™). Lactate dehydrogenase (LDH) was performed for cytotoxicity. The compounds, 4, 6, and 7 displayed the strong antiproliferative activity against corresponding cell lines. Apigenin-7-O-β-glucoside (4) revealed the excellent activity on HeLa, HT29, A549, MCF6 cancer cell lines with the values of (IC 50, µM) 167.3, 196.8 181.1, 203.5 respectively compared standard compound, cisplatin.

Chemopreventive Effects of Onosma mutabilis against Azoxymethane-Induced Colon Cancer in Rats via Amendment of Bax/Bcl-2 and NF-κB Signaling Pathways

“…The significant biological activity of OME was linked with the plant's antioxidant potential (1.12-2.33 mg/mL) possessed by its phytochemical contents, alkaloids (78.77%) and steroids (11.48%), especially 5,8-dihydroxy-2-(4-methylpent-3-enyl) naphthalene-1,4-dione, 3-O-Methyl-d-glucose, and β-Sitosterol as shown by the GC-MS analysis [16]. Similarly, researchers have shown experimental data results on the anticancer activity of Onosma species, Onosma stellulata [27], Onosma bracteata [15], and Onosma bourgaei [14]. Previously, scientists have shown the anticancer potential (against HCT 116 cell) of naphthoquinone (β-hydroxyisovaleryl shikonin) at the sub-G1 phase through its facilitating roles in the production of the reactive oxygen species (ROS) and inducing cancer cell apoptosis by caspase8/9 stimulation [28].…”

“…The genus Onosma is a group of well-known traditional medicinal plants that contain numerous phytochemicals offering different bioactivity against a wide range of moderate and severe human diseases [12,13]. The identified phytochemicals (rosmarinic acid, apigenin-7-O-β-glucoside, and globoidnan A) from O. bourgaei showed significant anticancer efficiency against human colorectal adenocarcinoma HT-29 and human mammary gland adenocarcinoma MCF-7 cells [14]. Furthermore, O. bracteata Wall was identified with different phytochemicals (1,2-benzene dicarboxylic acid and bis (2-methyl propyl) ester) and its aerial part extracts were found to be significant growth inhibitors of human lung carcinoma A549 cell, human osteosarcoma MG-63, and human neuroblastoma IMR-32 [15].…”

Ethnobotanical, Phytochemistry, and Pharmacological Activity of Onosma (Boraginaceae): An Updated Review

“…For the past decades, several studies have confirmed the traditional usage of the Onosma species as cytotoxic agents, and mammalian cancer cell division was inhibited by its extracts and isolated compounds [45,55,60].…”

“…The methanol extract of O. mutabilis aerial parts indicated significant anticancer activity against prostate (DU-145), mammary (MCF-7), and cervical cancer (Hep2c) cells with IC 50 values as 35.67 ± 0.15, 28.79 ± 0.23, and 41.83 ± 0.21 µg/mL, respectively [55]. The crude extracts of O. aucheriana showed significant cytotoxicity activity against human rhabdomyosarcoma, human cervix carcinoma Hep2c, and from murine fibroblast (L2OB) cell lines with IC 50 values range between 25.54 to 50.57 µg/mL [45].…”

Rosmarinic Acid and Related Dietary Supplements: Potential Applications in the Prevention and Treatment of Cancer

“…The anti-proliferative ability of RA was improved at lower concentrations in combination therapy [ 11 , 101 ]. RA behaved the excellent anti-proliferative activity against HeLa, HT29, A549 and MCF6 cancer cell lines with the IC 50 values of 249.80, 277.85, 241.47, and 220.25 µM [ 102 ]. EGFR is a primary target of the anti-proliferation effects of RA [ 80 , 101 ].…”