Antiproliferative and Antioxidant Effects of Withania coagulans Extract on Benign Prostatic Hyperplasia in Rats

Sarbishegi, Maryam; Khani, Mohaddeseh; Salimi, Saeedeh; Valizadeh, Mohharam; Aval, F Sargolzaei

doi:10.5812/numonthly.33180

Cited by 25 publications

(32 citation statements)

References 35 publications

(36 reference statements)

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“…A recent study showed that WCE increased the total antioxidant capacity (TAC) in benign prostatic hyperplasia in rats (36). These data are in line with the finding of our study indicating that pretreatment with WCE for 30 days improved the mean of SOD and CAT activity in WCE group when compared to I/R group.…”

Section: Discussionsupporting

confidence: 82%

Withania coagulans Protects Striatum from Oxidative Damages Induced by Global Brain Ischemia in Rat

Mahmoudzadeh‐Sagheb

Heidari

Sarbishegi

2017

Jundishapur J Nat Pharm Prod

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Background: Withania coagulans is a traditional plant that possesses diverse pharmacological characteristics including antihyperglycemic, anti-hyperlipidemic, antitumor, anti-inflammatory, and antioxidant effects. Objectives: This study evaluated the effect of preischemic administration of Withania coagulans extract (WCE) on antioxidant status and histopathological alteration of the striatum region in rat model of brain global ischemia reperfusion (I/R). Methods: 48 male Wistar rats were divided into four groups (n = 12): Control, Sham operated (Sham), I/R, and WCE + I/R. The animals received WCE or distilled water by oral gavage for 30 days before I/R except the control group. I/R was induced by two common carotid obstructions for 30 minutes. Three days following I/R, the animals were sacrificed, the brain was removed, and striatum was dissected out. Antioxidant statuses such as superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) activities, and malondialdehyde (MDA) level were measured and histological alteration was investigated in corpus striatum by Nissl and TUNEL staining. Results:The results showed that the CAT, SOD, and GPx activities decreased and MDA level increased in I/R group compare to the control group. Preischemic administration of WCE significantly decreased MDA level and increased antioxidant statuses in the striatum region compared to I/R group (P < 0.001). Histopathological analysis revealed that pretreatment with WCE markedly decreased necrotic and apoptotic neurons (P < 0.001) in striatum and reduced white matter injury induced by I/R. Conclusions: We concluded that WCE by its antioxidant and anti-inflammatory effects could modify ischemia reperfusion damage in rat striatum.

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Section: Discussionsupporting

confidence: 82%

Withania coagulans Protects Striatum from Oxidative Damages Induced by Global Brain Ischemia in Rat

Mahmoudzadeh‐Sagheb

Heidari

Sarbishegi

2017

Jundishapur J Nat Pharm Prod

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“…Antioxidant enzymes function as scavengers of free radicals by converting them to less harmful oxygen species [26]. Higher activities of antioxidant enzymes have an antioxidant effect by the alleviation of oxidative stress [7,25]. PFE treatment significantly the increased activities of antioxidant enzymes responsible for the reduction of oxidative damage in prostatic tissue induced by testosterone.…”

Section: Discussionmentioning

confidence: 99%

Inhibitory effects of Ponciri Fructus on testosterone-induced benign prostatic hyperplasia in rats

Jeon

Ohn

Seo

et al. 2017

65th International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research (GA 2017)

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Background: Benign prostatic hyperplasia (BPH) is non-cancerous condition of enlargement of the prostate, a common occurrence in older men. The immature fruits of Poncirus trifoliata (L.) Rafinesque (Rutaceae), Ponciri Fructus are widely used in traditional oriental medicine for the therapy of various diseases. However, little is known about the mechanism underlying the pathogenesis of BPH. In the present study, we investigated the protective effects of a Ponciri Fructus extract (PFE) on the development of BPH in a in a rat model of BPH induced by testosterone propionate (TP). Methods: Male Sprague Dawley rats were used as a model of BPH after its induction by daily subcutaneous injections of TP/corn oil, for a period of four weeks. PFE was administrated daily 1 h before TP/corn oil injection by oral gavage at a dose level of 200 mg/kg during the 4 weeks of TP/corn oil injections. All rats were sacrificed at the end of the experiment, we measured the relative prostate weight, the levels of testosterone and dihydrotestosterone (DHT), histological changes, activities of antioxidant enzymes (catalase, glutathione peroxidase, glutathione reductase, and superoxide dismutase), and expression of proliferating cell nuclear antigen (PCNA). In addition, we also measured the inhibition (%) of 5α-reductase in the prostatic tissue.

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“…In our previous study, we showed that WC extract (WCE) improved the prostate antioxidant status and decreased the stromal cell proliferation in a rat BPH model (14). Our aim in the present study was to evaluate the effects of WCE on COX-2 expression and apoptosis in a rat model of BPH.…”

Section: Objectivesmentioning

confidence: 99%

“…Withania coagulans (WC) is a traditional medicine from the Solanaceae family that is used for the treatment of various health problems and diseases in South Asia and the southeast regions of Iran (13). WC is well known for its antioxidant, anti-inflammatory, antiproliferative, and anti-cancerous properties (14-16). It has been reported that Withaferin A, one of the bioactive components of Withania, induces apoptosis in prostate cancer cells (17).…”

Section: Introductionmentioning

confidence: 99%

Withania coagulans Extract Induces Cell Apoptosis and Inhibits COX-2 Expression in a Rat Model of Benign Prostatic Hyperplasia

2016

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BackgroundPhytotherapy is a popular treatment option in cases of benign prostatic hyperplasia (BPH), with many different herbal products being used for the treatment of this condition. Withania coagulans (WC) is an herbal medicine that has shown anti-tumoral, anti-inflammatory, and antioxidant effects.ObjectivesThis study examined the effect of Withania coagulans extract (WCE) on prostatic cell apoptosis and cyclooxygenase-2 (COX-2) expression in cases of benign prostatic hyperplasia (BPH) in rats.MethodsForty Wistar rats were equally divided into five groups: control, sham, BPH, BPH + WCE, and BPH + CLX (celecoxib) as a positive control group. The induction of BPH was achieved via the subcutaneous injection of 3 mg/kg of testosterone propionate (TP) daily for 28 days. The animals received WCE, celecoxib, or distilled water by oral gavage accompanied by the TP injection. After four weeks, the prostate glands of the rats were weighed to measure the prostatic index (PI). The ventral lobes of the prostates were dissected and processed with paraffin blocks in order to study the number of mast cells. A TUNEL analysis was performed to evaluate the cell apoptosis, while the expression of COX-2 was examined using immunohistochemistry.ResultsBPH was obvious in the ventral lobe of the prostate, and the administration of WCE markedly decreased the PI and the number of mast cells (P < 0.001) in the BPH rats. Additionally, the WCE treatment induced prostatic cell apoptosis when compared to the BPH group. Furthermore, following the WCE treatment, the expression of COX-2 in the prostatic tissues was significantly decreased when compared to the BPH groups.ConclusionsAccording to the results of this study, WCE was effective in the treatment of BPH in rats. It may therefore have beneficial effects in the treatment of patients with BPH.

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Antiproliferative and Antioxidant Effects of Withania coagulans Extract on Benign Prostatic Hyperplasia in Rats

Cited by 25 publications

References 35 publications

Withania coagulans Protects Striatum from Oxidative Damages Induced by Global Brain Ischemia in Rat

Withania coagulans Protects Striatum from Oxidative Damages Induced by Global Brain Ischemia in Rat

Inhibitory effects of Ponciri Fructus on testosterone-induced benign prostatic hyperplasia in rats

Withania coagulans Extract Induces Cell Apoptosis and Inhibits COX-2 Expression in a Rat Model of Benign Prostatic Hyperplasia

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