Antioxidative responses in zebrafish liver exposed to sublethal doses Aphanizomenon flos-aquae DC-1 aphantoxins

Zhang, De Lu; Liu, Si Yi; Zhang, Jing; Hu, Chun Xiang; Li, Dun Hai; Liu, Yong Ding

doi:10.1016/j.ecoenv.2014.12.029

Cited by 16 publications

(8 citation statements)

References 67 publications

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“…Their physiological complexity also provides conserved models of human disease for in vivo validation studies[10]. The biological characteristics of zebrafish are suitable for toxicity testing, including eco-toxicology[11]. However, the Clock gene involved in the central oscillation to coordinate endogenous rhythms is linked to the generation of circadian rhythms[12].…”

Section: Introductionmentioning

confidence: 99%

Flexible and waterproof micro-sensors to uncover zebrafish circadian rhythms: The next generation of cardiac monitoring for drug screening

Zhang

Beebe²,

Jen³

et al. 2015

Biosensors and Bioelectronics

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Flexible electronics are the next generation of sensors for mobile health and implantation. Zebrafish (Danio rerio) is an emergent strategy for pre-clinical drug development and toxicity testing. To address the confounding effects from sedation of fish and removal from the aquatic habitat for micro-electrocardiogram (μECG) measurements, we developed waterproof and wearable sensors to uncover the circadian variation in heart rate (HR) and heart rate variability (HRV)[1]. The parylene-C based ECG sensor consisted of an ultra-soft silicone integrated jacket designed to wrap around the fish during swimming. The Young’s modulus of this silicone jacket matched with the fish surface, and an extended parylene cable connected the underwater chest electrodes with the out-of water electronics. In addition, embedded micro-glass spheres in the silicone effectively reduced the effective density of the jacket to ~ 1 g·cm−3. These innovations enabled physiological ECG telemetry in the fish’s natural habitat without the need for sedation. Furthermore, a set of non-linear signal processing techniques filtered out the breathing and electromagnetic artifacts from the recorded signals. We observed a reduction in mean HR and an increase in HRV over 24 hours at 10 dpa, accompanied by QT prolongation as well as diurnal variations, followed by normalization in mean HR and QT intervals at 26 days post ventricular amputation (dpa). We revealed Amiodarone-mediated QTc prolongation, HR reduction and HRV increase otherwise masked by sedation. The novel features of the flexible silicon jacket for μECG telemetry unraveled the biological clock and normalization of QT intervals at 26 dpa, providing the first evidence of new physiological phenomena during cardiac injury and repair as well as cardiac drug-mediated aberrant rhythms. Thus, the light weight and waterproof design holds promise to advance the next generation of mobile health and drug discovery.

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Section: Introductionmentioning

confidence: 99%

Flexible and waterproof micro-sensors to uncover zebrafish circadian rhythms: The next generation of cardiac monitoring for drug screening

Zhang

Beebe²,

Jen³

et al. 2015

Biosensors and Bioelectronics

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“…It is known that microcystins and saxitoxins are able to induce oxidative stress in animals. Taking into account the results of antioxidant enzyme activity and fitness measures ( r ) of cladocerans, it seem reasonable to suppose that oxidative stress played a role in the decreased fitness in the cyanobacterial treatments.…”

Section: Discussionmentioning

confidence: 99%

Single and combined effects of microcystin‐ and saxitoxin‐producing cyanobacteria on the fitness and antioxidant defenses of cladocerans

Ferrão-Filho

Silva

Oliveira

et al. 2017

Enviro Toxic and Chemistry

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Cyanobacteria produce different toxic compounds that affect animal life, among them hepatotoxins and neurotoxins. Because cyanobacteria are able to produce a variety of toxic compounds at the same time, organisms may be, generally, subjected to their combined action. In the present study, we demonstrate the single and combined effects on cladocerans of cyanobacteria that produce microcystins (hepatotoxins) and saxitoxins (neurotoxins). Animals were exposed (either singly or combined) to 2 strains of cyanobacteria isolated from the same environment (Funil Reservoir, Rio de Janeiro, Brazil). The effects on clearance rate, mobility, survivorship, fecundity, population increase rate (r), and the antioxidant enzymes glutathione-S-transferase (GST) and catalase (CAT) were measured. Cladoceran species showed a variety of responses to cyanobacterial exposures, going from no effect to impairment of swimming movement, lower survivorship, fecundity, and general fitness (r). Animals ingested cyanobacteria in all treatments, although at lower rates than good food (green algae). Antioxidant defense responses were in accordance with fitness responses, suggesting that oxidative stress may be related to such effects. The present study emphasizes the need for testing combined actions of different classes of toxins, because this is often, and most likely, the scenario in a more eutrophic world with global climatic changes. Environ Toxicol Chem 2017;36:2689-2697. © 2017 SETAC.

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“…Previous research has demonstrated that PSPs could be absorbed into the body of fish through the consumption of PSPpolluted foodstuffs (Jiang et al, 2007). Previous studies in fish have reported that PSPs are absorbed, accumulate in the muscle, influence development, cause deformation and death of larval fish, affect swimming ability, and result in changes in the structure and function of the brain and liver, demonstrating that PSPs or aphantoxins are toxic to fish (Samson et al, 2008;Bakke et al, 2010;Zhang et al, 2013aZhang et al, ,b,c, 2014Zhang et al, , 2015.…”

Section: Introductionmentioning

confidence: 99%

Respiratory toxicity of cyanobacterial aphantoxins from Aphanizomenon flos-aquae DC-1 in the zebrafish gill

et al. 2016

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Antioxidative responses in zebrafish liver exposed to sublethal doses Aphanizomenon flos-aquae DC-1 aphantoxins

Cited by 16 publications

References 67 publications

Flexible and waterproof micro-sensors to uncover zebrafish circadian rhythms: The next generation of cardiac monitoring for drug screening

Flexible and waterproof micro-sensors to uncover zebrafish circadian rhythms: The next generation of cardiac monitoring for drug screening

Single and combined effects of microcystin‐ and saxitoxin‐producing cyanobacteria on the fitness and antioxidant defenses of cladocerans

Respiratory toxicity of cyanobacterial aphantoxins from Aphanizomenon flos-aquae DC-1 in the zebrafish gill

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