Antioxidative effects of α-lipoic acid in the brain, liver and kidneys in selected mouse organs exposed to zymosan

Abstract

This study investigated the role of exogenous α-lipoic acid (ALA) in the inflammation caused by zymosan application. Seventy-two adult male white mice were divided into twelve groups: three control groups, three Zymosan groups, three ALA groups and three groups being the combination of Zymosan and ALA. In the experimental groups, the animals were decapitated after 3, 6 and 24 hours after the injection. The activity of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) were determined in the brain, liver and kidneys of the mice. After the injection of Zymosan, it was found that the activity of SOD, CAT and GSH-Px in the brain, liver and kidneys of mice was significantly lower in all time periods. The administration of ALA resulted in an opposite effect, namely, it increased the activity of the enzymes studied in the selected organs of mice. The Zymosan and ALA combination significantly inhibited the decrease in the activity of the enzymes compared with the values obtained in the groups of animals which received Zymosan only. The results of our study, using the Zymosan-induced inflammation, clearly indicate that ALA is an anti-inflammatory agent.

Protective effect of alpha‐lipoic acid, aerobic or resistance exercise from colitis in second hand smoke exposed young rats

“…19 In recent studies of sepsis and organ damage models, ALA application was reported to ameliorate the damage in liver and kidney by decreasing inflammatory parameters 7 and by increasing antioxidant levels. 20 Our results are in accordance with previous studies which examined the anti-inflammatory and antioxidant effects of ALA, however, in our study antioxidant GSH levels were not increased via ALA application. Besides, our research aimed F I G U R E 4 (A) Luminol and (B) lucigenin-enhanced chemiluminescence levels in colon tissue of control or colitis-induced rats, which were either sedentary or performed aerobic or resistance exercise for 5 weeks before colitis induction, which were either vehicle or α-lipoic acid (ALA)-treated and which were exposed to second hand smoke or not.…”

“…While there is little research indicating the effect of ALA on colitis, it was shown to be effective on Helicobacter pylori ‐induced gastritis . In recent studies of sepsis and organ damage models, ALA application was reported to ameliorate the damage in liver and kidney by decreasing inflammatory parameters and by increasing antioxidant levels . Our results are in accordance with previous studies which examined the anti‐inflammatory and antioxidant effects of ALA, however, in our study antioxidant GSH levels were not increased via ALA application.…”

D-Galactosamine Causes Liver Injury Synergistically with Lipopolysaccharide but not Zymosan in Chicks

“…LPS increases hepatic TBARS levels and decreases SOD activity in rats[ 4 ]. In mice, IP injection of zymosan also decreases hepatic SOD activity[ 12 ]. Moreover, in mice LPS combined with D-GalN (1 and 300 mg/kg) increased TBARS levels and decreased SOD activity[ 9 ].…”

“…Similarly, Cuzzocrea et al[ 11 ] demonstrated that zymosan did not induce severe liver injury in inducible NO synthase (iNOS)-deficient mice compared to that in wild-type mice. Additionally, oxidative stress may mediate PAMP-induced organ injury, because lipid peroxidation is increased by treatment with PAMPs[ 4 , 9 , 12 ].…”