Antioxidative and hepatoprotective activities of a novel polysaccharide (LSAP) from Lepista sordida mycelia

Xu, Yingyin; Li, Yuanhui; Lu, Yuxiao; Feng, Xiao; Gao, Tian; Liu, Qinghong

doi:10.1016/j.fshw.2021.04.016

Cited by 15 publications

(11 citation statements)

References 35 publications

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“…It has been shown that polysaccharides can enhance antioxidant activity in immunosuppressed mice (26). Our research found that F. velutipes polysaccharide could reduce the level of MDA and alleviate oxidative stress in the body by increasing the activity of SOD, CAT and T-AOC, which is consistent with the results of Xu et al (56).…”

Section: Discussionsupporting

confidence: 92%

The Effect of Flammulina velutipes Polysaccharide on Immunization Analyzed by Intestinal Flora and Proteomics

et al. 2022

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Proteomics and intestinal flora were used to determine the mechanism of immune modulatory effects of Flammulina velutipes polysaccharide on immunosuppressed mice. The results showed that compared with the model group, F. velutipes polysaccharide could increase thymus and spleen indices and improve thymus tissue structure in mice; IL-2 and IL-4 contents were significantly increased and IL-6 and TNF-α contents were significantly decreased; serum acid phosphatase (ACP), lactate dehydrogenase (LDH) and total antioxidant capacity (T-AOC) activities were increased (P < 0.05); in the liver, superoxide dismutase (SOD) and catalase (CAT) activities were increased (P < 0.001), while malondialdehyde (MDA) content was decreased (P < 0.001). Proteomics discovered that F. velutipes polysaccharides may exert immune modulatory effects by participating in signaling pathways such as immune diseases, transport and catabolism, phagosomes and influenza A, regulating the immune-related proteins Transferrin receptor protein 1 (TFRC) and Radical S-adenosyl methionine domain-containing protein 2 (RSAD2), etc. Gut microbial studies showed that F. velutipes polysaccharides could increase the abundance of intestinal flora and improve the flora structure. Compared to the model group, the content of short-chain fatty acids (SCFAs) and the relative abundance of SCFA-producers Bacteroides and Alloprevotella were increased in the F. velutipes polysaccharide administration group, while Lachnospiraceae_NK4A136_group and f_Lachnospiraceae_Unclassified decreased in relative abundance. Thus, F. velutipes polysaccharide may play an immunomodulatory role by regulating the intestinal environment and improving the balance of flora.

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Section: Discussionsupporting

confidence: 92%

The Effect of Flammulina velutipes Polysaccharide on Immunization Analyzed by Intestinal Flora and Proteomics

et al. 2022

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“…The FVPs were passed through a Shiseido C18 column at room temperature at a flow rate of 1 ml/min. The saccharides were analyzed using the standard method outlined by the National Renewable Energy Laboratory (CO, United States): hydrolysis with dilute sulfuric acid together with calibration using standard solutions of xylose, rhamnose, fucose, mannose, arabinose, galactose, galacturonic acid, glucose, glucuronic acid, and ribose ( Xu et al, 2021a ). The weight-average (Mw) and number-average (Mn) molecular weights of the FVPs were determined via high-performance gel permeation chromatography, as previously described in the literature ( Xu et al, 2021a ).…”

Section: Methodsmentioning

confidence: 99%

“…The saccharides were analyzed using the standard method outlined by the National Renewable Energy Laboratory (CO, United States): hydrolysis with dilute sulfuric acid together with calibration using standard solutions of xylose, rhamnose, fucose, mannose, arabinose, galactose, galacturonic acid, glucose, glucuronic acid, and ribose ( Xu et al, 2021a ). The weight-average (Mw) and number-average (Mn) molecular weights of the FVPs were determined via high-performance gel permeation chromatography, as previously described in the literature ( Xu et al, 2021a ). Fourier transform infrared (FT-IR) spectra of the FVPs were captured using an FT-IR microscope (model iN 10, Thermo Nicolet Corp.; Madison, WI, United States) fitted with a liquid nitrogen-cooled mercury-cadmium-telluride detector ( Xu et al, 2021b ).…”

Section: Methodsmentioning

confidence: 99%

Flammulinavelutipes Polysaccharides Modulate Gut Microbiota and Alleviate Carbon Tetrachloride-Induced Hepatic Oxidative Injury in Mice

Zhang

Wang

et al. 2022

Front. Microbiol.

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A carbon tetrachloride-induced acute liver injury mouse model is used to study the regulation of gut microbiota and hepatoprotective effect of polysaccharides from Flammulina velutipes (FVPs). The hepatoprotective effect of the FVPs leads to reduced levels of serum aspartate transaminase (AST), alanine aminotransferase (ALT), triglyceride (TG), total cholesterol (TC), total bile acid (TBA) content, and change in liver histopathology. Their anti-oxidant activity is exhibited by decreased levels of hepatic malonaldehyde (MDA) and protein carbonyl (PC) content and increased catalase (CAT) and superoxide dismutase (SOD) content. The anti-inflammatory ability of the FVPs is reflected in a decrease in pro-inflammatory cytokines (including IL-6, IL-1β, and TNF-α). 16S rRNA sequencing shows that the FVPs change the composition of the gut microbiota. A subsequent metabolomics analysis of the gut bacteria (UHPLC–MS/MS-based) revealed that fatty acid biosynthesis, tryptophan metabolism, and metabolism of xenobiotics by cytochrome P450 play important roles in the hepatoprotective effect. This study provides a potential way to modulate gut microbiota and manage liver diseases using natural products.

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“…The low EC 50 values for these radicals are 30 and 330 μg/ml, respectively (Murcia et al, 2002). Therefore, from these data, it can be inferred that the species of Lepista exhibit high antioxidative activity and L. sordida in particular (Kandaswami & Middleton, 1997) have the potency to treat age‐related disorders in mice and human model systems (Xu et al, 2021). Thereby both species mark their rich prospects in the field of therapeutic applications (Zhong et al, 2013).…”

Section: Prospective Medicinal Attributesmentioning

confidence: 99%

A review on Blewit mushrooms ( Lepista sp.) transition from farm to pharm

Nandi

Acharya

2022

Food Processing Preservation

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Mushrooms are an emerging natural resource to meet the current demands of functional food and therapeutics. Modern pharmacological as well as clinical trials of new‐age drugs have presented the nutritional and medicinal attributes of mushrooms in light. One such resource is the genus Lepista of the family Tricholomataceae, which is yet to be explored wholly. In recent years, the abandoned taxon Lepista has been in focus for the reason of its palatability, farming procedure, and curative importance. The key message of the review is to update the present status of the genus Lepista with reference to its significance as functional food, a new source of bioactive components, and therapeutic value, which will put forward beneficial guidance and new approaches for its advancement as a potent mycomedicine. Practical applications Application of mushrooms as food and medicine was conducted from the nutritional contents point of view and as medicinal value. In this field, the species of Lepista is globally admired but they are still largely unexplored. The present findings illustrate the promising nutritional and medicinal benefits of Lepista nuda and Lepista sordida. Additionally, the cultivation process of this species provides the people with additional vegetables of high quality and enriches the diet, which can be of direct benefit to human health and fitness.

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Antioxidative and hepatoprotective activities of a novel polysaccharide (LSAP) from Lepista sordida mycelia

Cited by 15 publications

References 35 publications

The Effect of Flammulina velutipes Polysaccharide on Immunization Analyzed by Intestinal Flora and Proteomics

The Effect of Flammulina velutipes Polysaccharide on Immunization Analyzed by Intestinal Flora and Proteomics

Flammulinavelutipes Polysaccharides Modulate Gut Microbiota and Alleviate Carbon Tetrachloride-Induced Hepatic Oxidative Injury in Mice

A review on Blewit mushrooms ( Lepista sp.) transition from farm to pharm

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