Antioxidant status and physiological responses of wheat (Triticum aestivum L.) to cycocel application and bio fertilizers under water limitation condition

Abstract

In order to study antioxidant status and physiological responses of wheat to cycocel (CCC) and bio fertilizers application under water limitation condition, a factorial experiment was conducted based on randomized complete block design with three replications in 2015. Treatments included water limitation in three levels [normal irrigation (I 1 ) as control; moderate water limitation (I 2 ) or irrigation withholding at 50% of heading stage; severe water limitation (I 3 ) or irrigation withholding at 50% of booting stage]; four bio fertilizer levels [(no bio fertilizer (F 0 ), seed inoculation by Azotobacter chrocoocum strain 5 (F 1 ), Pseudomonas putida strain 186 (F 2 ), Azotobacter + Pseudomonas (F 3 ))] and four CCC levels [(without CCC as control (C 0 ), application of 400 (C 1 ), 800 (C 2 ) and 1200 (C 3 ) mg/l)]. The results showed that water limitation decreased the chlorophyll-a, chlorophyll-b, total chlorophyll, carotenoid, stomata conductance, leaf area index (LAI) and relative water content of wheat, but activity of catalase (CAT), peroxidase (POD), polyphenol oxidase (PPO) enzymes and proline content were increased. Similar results were observed in CAT, POD and PPO activities due to bio fertilizers and CCC application. Besides the water limitation effects, CCC-treated plants displayed a significant decrease in stomata conductance and LAI. Generally, it was concluded that the application of bio fertilizers and CCC can be a proper tool for increasing wheat yield under water limitation. ARTICLE HISTORY

Pigments photosynthétiques, enzymes antioxydantes et potentiel osmotique foliaire de dix génotypes de blé dur (Triticum durum) : effet du stress hydrique

“…Outre les dommages causés à la chlorophylle, le stress hydrique réduit également la concentration des Car (Kiani et al 2008), essentiel- lement par la production accentuée de ROS dans les thylakoïdes (Reddy et al 2004). Des résultats similaires ont été obtenus chez de nombreuses espèces, tels que le riz (Oryza sativa L.) (Cha-Um et al 2010), le blé tendre (Triticum aestivum) (Hammad et Ali 2014;Khalilzadeh et al 2016) et le blé dur (Chahbar et Belkhodja 2016), en réponse au stress hydrique. Les Car jouent un rôle très important dans la tolérance à la sècheresse, en dissipant l'excès d'énergie sous forme de chaleur et en protégeant la plante des dommages oxydatifs (Gill et Tuteja 2010).…”

Effect of bacterization with Aeromonas media GS4 and Pseudomonas extremorientalis PhS1 on wheat seedlings under different abiotic conditions

“…In the West Siberia, abiotic factors that are the most stress-inducing for plants include low temperatures and high soil moisture at the beginning of crop vegetation and, on the contrary, extremely high temperatures and drought during budding and flowering. Abiotic stresses cause a number of morphological, physiological and molecular changes; disturb energy metabolism and regulation systems, which influence the growth and productivity of plants, as well as can lead to their death [4][5][6]. Under the influence of stress factors, both non-specific plant responses and production of specific compounds were observed [7][8][9][10].…”

“…When plants are subjected to stresses for a long time, protective systems may fail to cope with their functions, and the balance between ROS formation and quenching is disturbed, which results in oxidative damage. The main enzymes protecting from the damaging effect of hydrogen peroxide in the plant organism are catalase and peroxidase mobilizing it to H 2 O [4,6]. In a living cell, peroxidases play a key role in keeping molecules in a reduced state, which is one of the main conditions for normal existence of living organisms [8,11].…”

“…Thus, the main function of peroxidases is to protect a plant organism from a negative impact of ROS forming during photosynthesis and respiration [10][11]. The compensatory effect of peroxidases at the level of ROS during its increasing under the influence of various stress factors should be highlighted [4][5][10][11][12], and the activity dynamics of these enzymes correlates with the development of plant resistance to abiotic stresses [4,6,8,11].…”

“…They show an increase in plant growth and biomass, induction of systemic resistance, and an increase in the activity of antioxidant enzymes in wheat, Arabidopsis, tomato and other plants when treated with Enterobacter ludwigii and Flavobacterium sp. [1], Azotobacter chrocoocum and Pseudomonas putida [6], Bacillus licheniformis [17], Bacillus amyloliquefaciens [3; 18], etc.…”