Antioxidant responses and oxidative stress after microcystin exposure in the hepatopancreas of an estuarine crab species

Pinho, Grasiela Lopes Leães; Rosa, C. Moura da; Maciel, Fábio Everton; Bianchini, Adalto; Yúnes, João; Proença, Luís Antônio de Oliveira; Monserrat, José M.

doi:10.1016/j.ecoenv.2004.11.014

Cited by 109 publications

(58 citation statements)

References 39 publications

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“…Antioxidant enzymes such as SOD, CAT, and GPx constitute the major defensive system against ROS (Sies, 1993), which alleviates the toxic effects of ROS by scavenging free radicals and ROS. Therefore, activation of antioxidant enzymes indicates the formation of ROS in the target organism (Pinho et al, 2005). On the other hand, oxidative damage in cytosol of cardiac muscle was evaluated with regard to LPO content as well, which is a widely recognized consequence of oxyradical production (Winston and Di Giulio, 1991).…”

Section: Discussionmentioning

confidence: 99%

The profound effects of microcystin on cardiac antioxidant enzymes, mitochondrial function and cardiac toxicity in rat

et al. 2009

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Section: Discussionmentioning

confidence: 99%

The profound effects of microcystin on cardiac antioxidant enzymes, mitochondrial function and cardiac toxicity in rat

et al. 2009

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“…Danos teciduais também associados ao estresse oxidativo induzido pela microcistina foram descritos por Pinho et al (2005) (GEHRINGER et al, 2003). Milutinovic et al (2003) relataram que os mecanismos responsáveis pela hepatotoxicidade aguda causada pela microcistina em ratos (Rattus norvegicus), foram também responsáveis pela nefrotoxicidade crônica observada nestes animais.…”

Section: Fisiopatologia Da Intoxicação Por Microcistinaunclassified

“…Em organismos aquáticos a exposição pode ocorrer por via oral pela ingestão de cianobactérias ou pela absorção de toxinas extracelulares, presentes na água (CARBIS et al, 1997;PINHO et al, 2005).…”

Section: Exposição àS Cianotoxinas E Sinais Clínicosunclassified

“…Pesquisas têm demonstrado o perigo da bioacumulação de microcistina em invertebrados e vertebrados aquáticos, incluindo os peixes, que participam da cadeia alimentar humana (MAGALHÃES; SOARES; AZEVEDO, 2001;MAGALHÃES et al, 2003;FIGUEIREDO et al, 2004;PINHO et al, 2005;XIE et al, 2007). Mohamed, Carmichael e Hussein (2003) relataram o acúmulo de microcistina no intestino, no fígado e no rim de tilápias (O. niloticus).…”

Section: Bioacumulação De Microcistinaunclassified

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Hepatotoxicidade associada à microcistina

Santos¹,

Bracarense

2008

Sem. Ci. Agr.

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ResumoEfluentes industriais e urbanos e a intensa exploração agrícola e de pescado têm levado à eutrofização de muitos mananciais de água, destinados ao consumo e às atividades recreacionais. A eutrofização das águas, freqüentemente, tem como conseqüência o desenvolvimento expressivo de cianobactérias. Estas florações induzem a sérios problemas, visto que a ocorrência de Microcystis aeruginosa, uma das cianobactérias mais difundidas, pode produzir microcistinas (MCs). O efeito citotóxico da MC tem sido descrito no fígado, pulmões, estômago e intestino. Mortes de seres humanos, de animais silvestres e domésticos têm sido associadas à exposição a MC. Esta pode ocorrer diretamente por ingestão, inalação, contato, inoculação intravenosa (hemodiálise) ou indiretamente, pelo consumo de animais, dentre os quais os peixes e moluscos, que podem ingerir as cianobactérias e suas toxinas. A mais tóxica e também mais comum das MCs é a microcistina-LR (MC-LR), cujo órgão alvo é o fígado. A MC chega ao fígado especificamente por transporte dos ácidos biliares e, uma vez no citoplasma, inibe as proteínas fosfatases 1 e 2A, induzindo ao aumento da fosforilação protéica. Esta reação tem duas conseqüências: destruição do citoesqueleto, causando efeitos citotóxicos e descontrole da divisão celular, levando à promoção tumoral. A exposição aguda à MC induz severa hemorragia intra-hepática, necrose e apoptose, enquanto a exposição crônica pode causar neoplasia hepática ou intestinal. Também tem sido relatado que a hepatotoxicidade da MC está intimamente associada à formação intracelular de espécies reativas de oxigênio. A MC pode ser naturalmente degradada por bactérias ou pela radiação solar. Contudo, se não houver eficiente degradação, a MC poderá persistir na cadeia alimentar aquática. A contaminação de águas pela MC representa, portanto, perigo à saúde humana e de animais. Assim, esforços para evitar a eutrofização de fontes de águas são essenciais para minimizar riscos à saúde pública. Palavras-chave: Microcystis aeruginosa, microcistina, cianotoxinas, hepatotoxina AbstractUrban and industrial discharges, intense agricultural exploitation and fisheries have been causing the eutrophication in both drinking and recreational waters. A frequent consequence of eutrophication in waters is the massive development of cyanobacteria. The occurrence of these blooms induces a severe problem, as Microcystis aeruginosa, the most widespread distributed cyanobacteria, can produce microcystins (MC). Toxic effects of MC have been described in liver, lungs, stomach, and intestine. Deaths in wildlife, livestock and human beings were also associated with MC exposition. MC exposition can occurs directly by ingestion, inhalation, contact, intravenous inoculation of contaminated water (hemodialysis) or indirectly, by the consumption of animals, as fish and mollusks, the majors ingestors of cyanobacteria and its toxins. The most 1 Prof. Ass.,

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“…The ROS are scavenging by the defense of antioxidant system which are antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), glutathione (GSH) peroxidase and GSH reductase, and non-enzymatic antioxidants such as reduced GSH [8]. When the antioxidant defense has not enough to remove the ROS or ROS are produced in a large quantity, they can stimulate oxidative damage resulting in lipid peroxidation, chromosome aberration and induce DNA damage through the 8-oxo-deoxyguanosine formation (8-oxo-dG) consider as a marker of oxidative DNA damage [9].…”

Section: Introductionmentioning

confidence: 99%

The Biochemical Alteration and Dna Damage in Rats (Rattus Rattus) After Chronic Intraperitoneally Injection to Purified Microcystin-Lr From Anabaena Circinalis

Khadairi

Al-amari

Almamoori

2017

Asian J Pharm Clin Res

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Objective: This study determined the effect of purified microcystin-leucine arginine (MC-LR) on biochemical and DNA damage parameters in rats.Methods: Utilization of preparative high-performance liquid chromatography in analysis, purification and collection of MC-LR, then intraperitoneally injection of purified MC-LR to rats. At the end of exposure, animals were sacrificed, and liver cell was isolated to measure the biochemical markers such as superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH) as well as measured malondialdehyde (MDA), reactive oxygen species (ROS) and cytochrome P450 (Cyt P450), and DNA damage markers such as comet length, tail length, and tail moment were measured with the single cell gel electrophoresis also called comet assay. Results:The present results showed significantly increased activities of SOD as well as concentration of MDA, ROS with increasing concentration of MC-LR but the activities of CAT and GSH, as well as Cyt P450, were significantly decreased with increasing MC-LR dose while makers of DNA damage such as comet length, tail length, and tail moment also significantly increased with increasing MC-LR dose. Conclusion:This study demonstrated that chronic exposure to MC-LR toxin can induce alteration of biochemical and DNA damage markers.

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Antioxidant responses and oxidative stress after microcystin exposure in the hepatopancreas of an estuarine crab species

Cited by 109 publications

References 39 publications

The profound effects of microcystin on cardiac antioxidant enzymes, mitochondrial function and cardiac toxicity in rat

The profound effects of microcystin on cardiac antioxidant enzymes, mitochondrial function and cardiac toxicity in rat

Hepatotoxicidade associada à microcistina

The Biochemical Alteration and Dna Damage in Rats (Rattus Rattus) After Chronic Intraperitoneally Injection to Purified Microcystin-Lr From Anabaena Circinalis

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