Antioxidant phenolic glucosides from Gentiana piasezkii

Abstract

An extract of Gentiana piasezkii afforded a new arbutin derivative 6'-O-vanilloylarbutin (1) and a new flavone-C-glucoside 7-O-feruloylorientin (2), together with four known flavonoids lutonarin (3), saponarin (4), isoorient (5) and luteolin (6). Their structures were established based on spectroscopic methods including 2D NMR (COSY and gHMBC) techniques. Compounds 1, 2, 5 and 6 were evaluated for the antioxidant activity in the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay system.

Phytochemical Investigation ofAnaphalis lactea

“…According to the reported procedures [3], the antioxidant activities of compounds 2, 4 -6 and 8 -16 were assessed on the basis of the radical scavenging effect of the stable DPPH free radical. Three milliliters of a 100 μM DPPH ethanol solution were added to 10 μL of sample solutions of different concentrations and allowed to react at room temperature.…”

“…Chemical structures of the isolated compounds.4,6-Dihydroxy-7-isobutyryl-5-prenyl-2(3H)-benzofuranone (4): White crystal (CHCl 3 ); m. p. 148 -150°C; UV (MeOH): λ max (log ε) = 240 (3.67), 280 (3.80), 332 (3.13) nm; [α] D -8 (c 0.2, CHCl 3 ); IR (KBr): ν max = 3453, 1803, 1630, 1604 cm -1 ; 1 H-NMR (CDCl 3 , 300 MHz): δ = 13.84 (brs, 6-OH), 6.60 (brs, 4-OH), 5.27 (1H, t, J = 6.6 Hz, H-2′), 3.70 (1H, sept, J = 6.6 Hz, H-9),3.62 (2H, s, H-3), 3.43 (2H, d, J = 6.6 Hz, H-1′), 1.84 (3H, s, H-5′), 1.79 (3H, s, H-4′), 1.21 (6H, d, J = 6.6 Hz, H-10,11); 13 C-NMR (CDCl 3 , 75 MHz): δ = 208.4 (s, C-8), 173.5 (s, C-2), 163.0 (s, C-6), 156.8 (s, C-4), 153.7 (s, C-7a), 137.2 (s, C-3′), 120.9 (d, C-2′), 109.1 (s, C-5), 100.7 (s, C-7), 100.4 (s, C-3a), 38.6 (d, C-9), 29.7 (t, C-3), 25.8 (q, C-4′), 21.8 (t, C-1′), 18.9 (q, C-10, 11), 17.9 (q, C-5′); EI-MS: m/z ( % rel. int.)…”

Use of lyophilised and powdered Gentiana lutea root in fresh beef patties stored under different atmospheres

“…The active properties of G. lutea have been reported several times by Aberham et al with commercial and fresh plants. The root compounds consist of iridoids, secoiridoids and xanthones which could be developed as antioxidant agents and radical scavengers and may contribute to the decrease of lipid oxidation . These compounds have been described as having antioxidant properties and are capable of scavenging free radicals and removing the superoxide radicals naturally produced in cells .…”

“…The root compounds consist of iridoids, secoiridoids and xanthones which could be developed as antioxidant agents and radical scavengers and may contribute to the decrease of lipid oxidation. 29 These compounds have been described as having antioxidant properties and are capable of scavenging free radicals and removing the superoxide radicals naturally produced in cells. 30 Recent studies have focused on evaluating the potential of natural plants to improve nutritional properties, to reduce lipid oxidation and to extend the shelf life of meat products.…”

Detection of the Genotoxicity of Gentiana L. Extracts by Using RAPD-PCR and ISSR-PCR Techniques

“…[5][6][7][8][9] Mostly the aerial parts and roots contain xanthones, free or glycosidic flavonoids, alkaloids, iridoids, secoiridoids and also triterpenoids. 4,[10][11][12] The clastogenic agents cause lesions in the genetic material. The capacity of them called as genotoxicity.…”