Detection of the Genotoxicity of Gentiana L. Extracts by Using RAPD-PCR and ISSR-PCR Techniques

Abstract

Backround: The RAPD-and ISSR-PCR techniques are offering an insight into the detecting the genotoxicity of Gentiana extracts. Objective: It is aimed with the present study, to detect the genotoxicity of methanol extracts of ten Turkish Gentiana L. taxa on germinated Allium cepa L. root tips. Methods: RAPD-and ISSR-PCR techniques were used for detection of genotoxicity of Gentiana extracts. Results: Four RAPD and three ISSR primers produced the reproducible polymorphic and monomorphic banding patterns among 10 RAPD and 10 ISSR primers for all DNA samples. It is not any serious alteration along with band intensity change, the disappearance of the bands, and appearance of the new bands in the band profiles amplified from the Gentiana extracts-treated genomic DNA sample of A. cepa. The most efficient results were obtained by RAPD-P9 and ISSR-1 primer among the seven productive primers. Discussion: It is not observed any variation in the RAPD-and ISSR-PCR band profiles in time and concentration-dependent manner. Conclusion: It is determined that the three different concentrations of Gentiana extracts did not interact with the A. cepa DNA.

Presence of arsenic and potentially toxic metals (Cd, Cr, Pb) in water and soil of the NE shore of Chapala Lake, Mexico, and its genotoxic effect in the edible chayote fruit (Sechium edule (Jacq.) Sw.)

“…In Allium cepa, ISSR techniques offered insight into detecting genotoxicity caused by Gentiana extracts (Poyraz et al, 2018). In the present study, the clustering corresponds to differences among sample sites and the detection of different concentrations of metals (Figure 3).…”

“…The displacement of DNA from the nucleus can be used as an indicator of damage (Jiang et al, 2014;Carbajal-López et al, 2015). Moreover molecular markers, such as ISSR (Inter Simple Sequence Repeat), have been used for the detection of DNA changes associated with abiotic factors (Bajpai et al, 2015;Kalubi et al, 2015) and genotoxicity (Al-Qurainy, 2010;Poyraz et al, 2018). Due to the importance of this crop for the region around Lake Chapala and the scarce studies for genotoxicity caused by some elements like metals in this crop, the objective of this study was to use comet assay to evaluate genotoxic damage and ISSR for genetic variation, which are caused by the presence of this pollutants in chayote crops cultivated on the NE shores of Lake Chapala.…”