Antioxidant peptides isolated from sea cucumber Stichopus Japonicus

Abstract

The sea cucumber Stichopus japonicus protein was hydrolyzed by pepsin, trypsin, papain, acid protease and neutral protease, respectively, to get five kinds of peptide fractions: pepsin peptides (PP), trypsin peptides (TP), acid protease peptides (AP), neutral protease peptides (NP) and papain peptide (PAP). Antioxidative activities of all peptide fractions were evaluated by hydroxyl radical-(ÁOH) and Superoxide anion (O 2 Á 2 )-scavenging activity.Trypsin peptide (TP) exhibited the highest antioxidative activity compared to other peptide fractions. In considering scavenging effects on hydroxyl radicals (ÁOH) and Superoxide anions (O 2 Á 2 ), TP was employed for isolation, purification and identification of antioxidant peptide. To purify and characterize antioxidative peptide, two steps gel filtration, one-step ion-exchange column chromatography and reversed-phase high-performance liquid chromatography (RP-HPLC) were used. The purified antioxidative peptide TP2b-1 was a novel peptide and was sequenced as GPEPTGPTGAPQWLR, in which the low molecular weight and some amino acid constituents played important role in the radical-scavenging effects according reports. The IC 50 values of TP2b-1 were 138.9 lM on ÁOH and 353.9 lM on O 2 Á 2 .

Protective effects against H2O2-induced oxidative damage in lung fibroblast cell by peptide isolated from plasma albumin hydrolysate

“…The antioxidative capability of peptides can be attributed to the long aliphatic side-chain groups of Leu, Ile and Val [46]. Ala also had the function of enhancing radical scavenging activity [47]. Secondly, the hydrophobicity of proteins or peptides provides easy access to hydrophobic targets and enhances the affinity and reactivity of peptides of the cell membrane in living cells [29,48].…”

Biological activities and biomedical potential of sea cucumber (Stichopus japonicus): a review

“…These oligopeptides exhibited protective barrier against hydroxyl radical-induced DNA damage at a concentration of 2 mg/ml. Zhou et al (2012) also studied the antioxidant activity of a novel peptide from S. japonicus by hydrolyzation with trypsin. The peptide was sequenced as GPEPTGPTGAPQWLR and IC 50 values of hydroxyl and superoxide radical scavenging activity were 138.9 and 353.9 μM, respectively.…”

“…The body wall of the sea cucumber is a major edible part and consists mainly of collagen and mucopolysaccharides (Duan et al 2010). In addition, the body wall with peptide, collagen, gelatin, polysaccharide, and saponin has several biological activities such as anticancer, anticoagulation, anti-oxidation, and anti-osteoclastogenesis (Kariya et al 2004;Lu et al 2010;Zhou et al 2012;Yang et al 2015a). Furthermore, sea cucumbers have high applications in the biomedical field because of their regenerative capacities as they can regenerate tissues and organs within a few months (Zohdi et al 2011).…”

Cosmeceuticals Properties of Sea Cucumbers: Prospects and Trends

“…Gelatin is a soluble protein obtained by partial hydrolysis of collagen, mainly from animal skin, bone, tendon, and cartilage [50]. So far, studies on sea cucumber collagen have been mainly focused on functions of hydrolytic bioactive peptides, including damaged tissue repairing [51], antitumor [52], antioxidant [53], and angiotensin-converting enzyme inhibitory activity [54,55]. Due to their antioxidant properties, collagen fibres have been used in skin care products [56,57].…”