Antioxidant enzymes expression in <i>Pseudomonas aeruginosa</i> exposed to UV‐C radiation

Kloula, Salma; Maalej, Lobna; Khefacha, Sana; Chourabi, Kalthoum; Imene, Ouzari; Chatti, Abdelwaheb

doi:10.1002/jobm.201500753

Cited by 10 publications

(4 citation statements)

References 18 publications

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“…For example, UV-C treatment has been shown to modify the cell structure in tomato fruit surface and thereby could play an important role in the resistance of infection (Charles et al 2008). UV-C has also been shown to increase the levels of reactive oxygen species (ROS) in both eukaryotic and prokaryotic organisms (Salma et al 2016), change the membrane fatty acid composition of cells (Ghorbal et al 2013), and enhance the antioxidant activity of products (Wu et al 2016). Thus, it can be concluded that UV-C pretreatment promotes the ability of stress resistance in potato tuber during cold storage.…”

Section: Expressions Of Sugar Metabolism Related Genesmentioning

confidence: 99%

UV-C treatment on physiological response of potato (Solanum tuberosum L.) during low temperature storage

et al. 2016

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The storage of potato tuber (Solanum tuberosum L.) at low temperatures minimizes sprouting and disease but can cause cold-induced sweetening (CIS), which leads to the production of the cancerogenic substance acrylamide during the frying processing. The aim of this research was to investigate the effects of UV-C treatment on CIS in cold stored potato tuber. 'Atlantic' potatoes were treated with UV-C for an hour and then stored at 4°C up to 28 days. The UV-C treatment significantly prevented the increase of malondialdehyde content (an indicator of the prevention of oxidative injury) in potato cells during storage. The accumulation of reducing sugars, particularly fructose and glucose, was significantly reduced by UV-C treatment possibly due to the regulation of the gene cascade, sucrose phosphate synthase, invertase inhibitor 1/3, and invertase 1 in potato tuber, which were observed to be differently expressed between treated and untreated potatoes during low temperature storage. In summary, UV-C treatment prevented the existence of oxidative injury in potato cells, thus, lowered the amount of reducing sugar accumulation during low temperature storage of potato tubers.

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Section: Expressions Of Sugar Metabolism Related Genesmentioning

confidence: 99%

UV-C treatment on physiological response of potato (Solanum tuberosum L.) during low temperature storage

et al. 2016

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“…Differences in the cell viability between strains were statistically significant (p < 0.05). These results suggest that strain I1P must have an efficient antioxidant defense mechanism that allows these cells to deal with ROS generation to prevent oxidative damage, particularly during the first stages of UV exposure [11,[51][52][53][54]. In order to study the antioxidant response to UV radiation, intracellular ROS levels were measured with the free radical probe H 2 DCFDA.…”

Section: Resultsmentioning

confidence: 99%

Isolation of a Psychrotolerant and UV-C-Resistant Bacterium from Elephant Island, Antarctica with a Highly Thermoactive and Thermostable Catalase

Monsalves¹,

Ollivet-Besson²,

Amenábar³

et al. 2020

Microorganisms

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Microorganisms present in Antarctica have to deal not only with cold temperatures but also with other environmental conditions, such as high UV radiation, that trigger the generation of reactive oxygen species. Therefore, Antarctic microorganisms must have an important antioxidant defense system to prevent oxidative damage. One of these defenses are antioxidant enzymes, such as catalase, which is involved in the detoxification of hydrogen peroxide produced under oxidative conditions. Here, we reported the isolation and partial characterization of an Antarctic bacterium belonging to the Serratia genus that was resistant to UV-C radiation and well-adapted to cold temperatures. This microorganism, denominated strain I1P, was efficient at decreasing reactive oxygen species levels produced after UV-C irradiation. Genomic and activity assays suggested that the enzymatic antioxidant defense mechanisms of strain I1P, especially its catalase enzyme, may confer UV resistance. This catalase was active in a wide range of temperatures (20–70 °C), showing optimal activity at 50 °C (at pH 7.0), a remarkable finding considering its psychrotolerant origin. In addition, this enzyme was thermostable, retaining around 60% of its activity after 6 h of incubation at 50 °C. The antioxidant defense systems of strain I1P, including its surprisingly thermoactive and thermostable catalase enzyme, make this microorganism a good source of biocompounds with potential biotechnological applications.

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“…This response is similar to that observed for three serovars of Salmonella: Salmonella Typhimurium, Salmonella Hadar, and Salmonella Zanzibar treated with UVc radiations (Maâlej et al, 2014). Therefore, UFAs are over-produced to scavenge the reactive oxygen species (ROS) and this overproduction is beneficial to ensure more radio-tolerance (Kloula et al, 2013, 2016). The relation between fatty acyl desaturation and bacterial adaptation was reported for a number of strains exposed to heat, cold and oxidative damage (Denich et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

Pseudomonas aeruginosa Swarmer Cells Adaptation Toward UVc Radiations

et al. 2019

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Swarming is the most rapid surface motility allowing Pseudomonas aeruginosa bacteria to rapidly colonize new surfaces. However, swarming behavior is affected by environmental factors like ultraviolet irradiation (UVc). UVc radiation is the most disinfection technology usually applied for wastewater and proven to be effective to inactivate microorganisms. However, efficiency against motile bacteria is not yet studied. This study aims to explain the mechanisms of resistance of swarmer P. aeruginosa cells toward UVc exposure. P. aeruginosa liquid cultures were allowed to swarm across a semisolid surface for 18 h and directly exposed to UVc radiations. Emergent swarmer colonies, revealed after re-incubation, were selected to study biofilm formation, fatty acid (FA) composition, and ultrastructure. Our results showed that membrane adaptation to UVc radiations was seen in Pseudomonas cells by an increase of cyclic fatty acid (CFA) content, confirming the role of cyclopropane in radio-resistance of swarmer cells. Furthermore, electron microscopic study confirmed that over production of S-layer is believed to be a protective form adopted by P. aeruginosa swarmer cells to resist after 5 min of UVc exposure. Moreover, membrane disintegration is the lethal effect observed after 15 min of UVc exposure. In the other hand, study of biofilm production showed an enhancement of biofilm formation, of swarmer cells mainly after 15 min of UVc exposure. There results confirmed that swarming process is highly correlated with particular FA composition of P. aeruginosa membrane and that radio-resistance of swarmer cells is highly supported by CFA biosynthesis and S-layer overproduction.

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Antioxidant enzymes expression in Pseudomonas aeruginosa exposed to UV‐C radiation

Cited by 10 publications

References 18 publications

UV-C treatment on physiological response of potato (Solanum tuberosum L.) during low temperature storage

UV-C treatment on physiological response of potato (Solanum tuberosum L.) during low temperature storage

Isolation of a Psychrotolerant and UV-C-Resistant Bacterium from Elephant Island, Antarctica with a Highly Thermoactive and Thermostable Catalase

Pseudomonas aeruginosa Swarmer Cells Adaptation Toward UVc Radiations

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