Pseudomonas aeruginosa Swarmer Cells Adaptation Toward UVc Radiations

Ghorbal, Salma Kloula Ben; Chourabi, Kalthoum; Maalej, Lobna; Ammar, Aouatef Ben; Ouzari, Hadda‐Imene; Hassen, A.; Jaafoura, H.; Chatti, Abdelwaheb

doi:10.3389/fmicb.2019.00556

Cited by 10 publications

(6 citation statements)

References 24 publications

(39 reference statements)

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“…Our results showed decreased levels of cy C17:0 in the swarmer cells of the ATCC 29906 and 1984 strains. Similar results were observed for swarmers of Caulobacter crescentus [48] and Pseudomonas aeruginosa [49]. Limitation of the cyclopropanation reaction seems likely to be important for the differentiation process of P. mirabilis elongated cells.…”

Section: Discussionsupporting

confidence: 78%

Adjustment in the Composition and Organization of Proteus mirabilis Lipids during the Swarming Process

Stolarek,

Bernat,

Różalski

2023

IJMS

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Proteus mirabilis, an opportunistic pathogen of the urinary tract, is known for its dimorphism and mobility. A connection of lipid alterations, induced by the rods elongation process, with enhanced pathogenicity of long-form morphotype for the development of urinary tract infections, seems highly probable. Therefore, research on the adjustment in the composition and organization of P. mirabilis lipids forming elongated rods was undertaken. The analyses performed using the ultra-high performance liquid chromatography with tandem mass spectrometry showed that drastic modifications in the morphology of P. mirabilis rods that occur during the swarming process are directly related to deprivation of the long-form cells of PE 33:1 and PG 31:2 and their enrichment with PE 32:1, PE 34:1, PE 34:2, PG 30:2, PG 32:1, and PG 34:1. The analyses conducted by the gas chromatography-mass spectrometry showed negligible effects of the swarming process on fatty acids synthesis. However, the constant proportions between unsaturated and saturated fatty acids confirmed that phenotypic modifications in the P. mirabilis rods induced by motility were independent of the saturation of the phospholipid tails. The method of the Förster resonance energy transfer revealed the influence of the swarming process on the melting of ordered lipid rafts present in the short-form rods, corresponding to the homogeneity of lipid bilayers in the long-form rods of P. mirabilis. Confocal microscope photographs visualized strong Rhod-PE fluorescence of the whole area of swarmer cells, in contrast to weak membrane fluorescence of non-swarmer cells. It suggested an increased permeability of the P. mirabilis bilayers in long-form rods morphologically adapted to the swarming process. These studies clearly demonstrate that swarming motility regulates the lipid composition and organization in P. mirabilis rods.

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Section: Discussionsupporting

confidence: 78%

Adjustment in the Composition and Organization of Proteus mirabilis Lipids during the Swarming Process

Stolarek,

Bernat,

Różalski

2023

IJMS

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“…K. intermedius OMVs from KMC, exposed at upper unprotected level of the sample carrier, had very low capacity to build the proton gradient. It should be noted that UV can act as a trigger for modulation of membrane structure modification, leading to either loss of implicated proteins or their suppression (Ghorbal et al, 2019). To sum up, Mars-like illumination, gravity, and atmosphere influenced outer membrane alterations, and UV, as a stressful factor, was the most pronounced for K. intermedius.…”

Section: Discussionmentioning

confidence: 97%

Fitness of Outer Membrane Vesicles From Komagataeibacter intermedius Is Altered Under the Impact of Simulated Mars-like Stressors Outside the International Space Station

et al. 2020

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Outer membrane vesicles (OMVs), produced by nonpathogenic Gram-negative bacteria, have potentially useful biotechnological applications in extraterrestrial extreme environments. However, their biological effects under the impact of various stressors have to be elucidated for safety reasons. In the spaceflight experiment, model biofilm kombucha microbial community (KMC) samples, in which Komagataeibacter intermedius was a dominant community-member, were exposed under simulated Martian factors (i.e., pressure, atmosphere, and UV-illumination) outside the International Space Station (ISS) for 1.5 years. In this study, we have determined that OMVs from post-flight K. intermedius displayed changes in membrane composition, depending on the location of the samples and some other factors. Membrane lipids such as sterols, fatty acids (FAs), and phospholipids (PLs) were modulated under the Mars-like stressors, and saturated FAs, as well as both short-chain saturated and trans FAs, appeared in the membranes of OMVs shed by both post-UV-illuminated and "dark" bacteria. The relative content of zwitterionic and anionic PLs changed, producing a change in surface properties of outer membranes, thereby resulting in a loss of interaction capability with polynucleotides. The changed composition of membranes promoted a bigger OMV size, which correlated with changes of OMV fitness. Biochemical characterization of the membrane-associated enzymes revealed an increase in their activity (DNAse, dehydrogenase) compared to wild type. Other functional membraneassociated capabilities of OMVs (e.g., proton accumulation, interaction with linear DNA, or synaptosomes) were also altered after exposure to the spaceflight stressors. Despite alterations in membranes, vesicles did not acquire endotoxicity, cytotoxicity, and neurotoxicity.

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“… 40 The relation between the swarming and biofilm formation is still unclear, so in a recent study, it was concluded that the swarm cells of P. aeruginosa become adapted to UV radiation and enhance the formation of biofilm. 41 In another study, it was discovered that due to exposure to kanamycin the swarming cells of Bacillus subtilis activate matrix genes and induces biofilm formation. 42 In the present findings, the EO from T. minuta inhibited the formation of biofilm as well as the swarming motility which was quite like previous findings.…”

Section: Discussionmentioning

confidence: 99%

Essential Oil from Tagetes minuta Has Antiquorum Sensing and Antibiofilm Potential against Pseudomonas aeruginosa Strain PAO1

Khan,

Waheed,

Azeem

et al. 2023

ACS Omega

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Biofilms are complex communities of microorganisms that are enclosed in a matrix that shows increased resistance to antimicrobial and immunological encounters. Mostly, the traditional methods to control biofilm are exhausted; therefore, the aim is to evaluate the potential of essential oil (EO) from Tagetes minuta to encounter biofilm and other related virulence factors. The EO of T. minuta was extracted through steam-distillation, analyzed on gas chromatography–mass spectrometry, and the biofilm inhibition assays were performed with various concentrations of EO. Mainly the EO from T. minuta contains cis-β-ocimene (29.1%), trans-tagetenone (23.1%), and cis-tagetenone (17.7%). The virulence factors were monitored while applying different concentrations of EO and it was recorded that the EO from T. minuta significantly inhibited the virulence factors linked with quorum sensing (QS), such as pyocyanin production, protease production, and swarming motility. Biofilm formation is one of the most important virulence factors associated with the QS pathway and was inhibited up to 79% in the presence of EO. Antibacterial activity against the PAO1 of EO was not so promising particularly and it has high MIC (325 μg/mL) and MBC (5000 μg/mL). EO is quite efficient to inhibit biofilm in a very small concentration of 20 μg/mL, which confirms that the biofilm inhibition by EO is not by killing bacterial cells but by inhibiting the QS pathway. The study on PAO1 constructs carrying various QS reported genes confirmed that the EO interferes with the QS pathway that ultimately controls various virulence factors caused by PAO1.

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Pseudomonas aeruginosa Swarmer Cells Adaptation Toward UVc Radiations

Cited by 10 publications

References 24 publications

Adjustment in the Composition and Organization of Proteus mirabilis Lipids during the Swarming Process

Adjustment in the Composition and Organization of Proteus mirabilis Lipids during the Swarming Process

Fitness of Outer Membrane Vesicles From Komagataeibacter intermedius Is Altered Under the Impact of Simulated Mars-like Stressors Outside the International Space Station

Essential Oil from Tagetes minuta Has Antiquorum Sensing and Antibiofilm Potential against Pseudomonas aeruginosa Strain PAO1

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