Antioxidant defenses at transcriptional and enzymatic levels and gene expression of Nrf2-Keap1 signaling molecules in response to acute zinc exposure in the spleen of the large yellow croaker Pseudosciaena crocea

Functional characterization and differential nutritional regulation of putative Elovl5 and Elovl4 elongases in large yellow croaker (Larimichthys crocea)

“…The stability of β-actin was verified and confirmed and used as the reference gene 53 . The amplification was performed in a quantitative thermal cycler (Mastercycler EP Realplex, Eppendorf, Germany).…”

Chronic waterborne zinc and cadmium exposures induced different responses towards oxidative stress in the liver of zebrafish

“…Thus, Zn and Cd toxicity is a well-studied topic in the field of aquatic toxicology. However, most studies have merely focused on the effects of the single Zn or Cd exposure on growth, survival, and oxidative stress in fish (Defo et al, 2014;Zheng et al, 2016aZheng et al, , 2016b. The molecular mechanisms that underlie these differences remain to be elucidated.…”

“…The complexity of antioxidant responses reflects a likewise complex mechanism of regulation, which involves different pathways modulating either the catalytic activity of pre-existing enzymes or the de novo synthesis of new molecules. Several studies reported the effects of metal exposure on the mRNA level and activity of antioxidant enzymes in fish and suggested a significant correlation between metal concentrations and the transcription level of some antioxidant genes (Craig et al, 2007;Giuliani and Regoli, 2014;Hansen et al, 2007;Zheng et al, 2016a;Zheng et al, 2016b). However, the changes of enzymes activities and gene transcription levels were inconsistent, suggesting that changes in gene transcription levels do not necessarily lead to modifications in the activity of corresponding enzymes (Giuliani et al, 2013).…”

“…Furthermore, numerous experimental evidences, mainly driven in mammalian models, demonstrated that antioxidant-related genes are under the control of numerous transcription factors activated by ROS, such as Nrf2 (NF-E2-related factor 2), which represents a critical mechanism of protection against chemically induced oxidative stress through Nrf2 signal molecule. In recent years, a few studies have demonstrated Nrf2 antioxidant defense induced by waterborne copper, zinc or cadmium in fish Wang and Gallagher, 5 2013; Zheng et al, 2016a). Thus, there may be a close relationship between metal induced oxidative stress and Nrf2, and Nrf2 may play an important role in modulating gene expression of antioxidant enzymes in fish, however, these warrant investigation.…”

Modulation of growth, immunity and antioxidant‐related gene expressions in the liver and intestine of juvenileSillago sihamaby dietary vitamin C

“…Nrf2 usually kept at a low level due to repression by Keap1 under normal physiological conditions (Jaiswal, ). Abundant studies have demonstrated that antioxidant gene (SOD, CAT, GPx, GST and GR) expression could be enhanced by activation of Nrf2 (Wang, Pan, Si, & Miao, ; Zhao et al, ; Zheng et al, ). To our knowledge, no research has studied Nrf2, Keap1 and antioxidant gene expression in S .…”