Chronic waterborne zinc and cadmium exposures induced different responses towards oxidative stress in the liver of zebrafish

Zheng, Jia; Yuan, Shu; Wu, Changwen; Li, Weiye

doi:10.1016/j.aquatox.2016.06.001

Cited by 96 publications

(22 citation statements)

References 51 publications

(51 reference statements)

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“…In this study, expression changes of cat, gstω1 and gpx1 in spleen as well as sod1, cat, gstω1 and gpx1 in head kidney were in parallel with alteration in nrf2 expression, suggesting that Cd 2+ affected antioxidant status of immune organs via Nrf2 signaling. Similarly, a positive correlation between nrf2 expression and the mRNA levels of antioxidant enzyme genes was also reported by other studies in fish after Cd exposure (Wang & Gallagher, 2013;Zheng, Yuan, Wu, & Li, 2016).…”

Section: Discussionsupporting

confidence: 85%

“…In this study, Cd-induced alterations of tgf-β expression in spleen as well as il-10 and tgf-β expression in head kidney were parallel with tor mRNA levels, probably implying that Cd altered the expression of anti-inflammatory cytokines by affecting the transcription of tor. Nevertheless, alteration of il-10 transcription level did not present similar tendency with tor in spleen, which could be related to other transcription factor such as NFκB that could also regulate anti-inflammatory expression (Li et al, 2016).…”

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confidence: 76%

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Sun¹,

Li²,

An³

et al. 2020

Turk. J. Fish. Aquat. Sci.

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Cadmium (Cd) is an environmental contaminant that presents significant threat to aquatic organisms. This study was conducted to examine histological structure as well as antioxidative and inflammatory responses in spleen and head kidney of yellow catfish after 8 weeks of exposure to 50 and 200 µg/L Cd 2+. It showed that Cd 2+ exposure induced Cd accumulation and impaired histological structure in the spleen and head kidney. For antioxidative response: in the spleen, sod1 expression was significantly upregulated by 50 and 200 µg/L Cd 2+ , while cat, gpx1, gstω1 and nrf2 expressions were up-regulated by 50 µg/L Cd 2+ but not by 200 μg/L Cd 2+ ; in the head kidney, these genes were down-regulated by Cd 2+ exposure. For inflammatory response: in the spleen, the expression of il-8, tnf-α and il-10 were up-regulated by 50 µg/L Cd 2+ but not by 200 μg/L Cd 2+ , while the expression of tor and tgf-β were significantly up-regulated only by 200 µg/L Cd 2+ ; in the head kidney, Cd 2+ exposure down-regulated the expression of il-1β, il-8, tor, tnf-α, il-10 and tgf-β. Therefore, this study suggested that Cd 2+ exposure could cause Cd accumulation in the spleen and head kidney, and induced histological lesions, antioxidative and inflammatory responses in both tissues.

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Section: Discussionsupporting

confidence: 85%

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confidence: 76%

Untitled

Sun¹,

Li²,

An³

et al. 2020

Turk. J. Fish. Aquat. Sci.

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“…Heavy metals are known to accumulate in fish tissues and catalyse reactions that generate reactive oxygen species (ROS) and lead to oxidative stress. In fish, antioxidant parameters can be induced or inhibited by heavy metals depending on the dose, the species, and the organ (Sanchez et al, 2005;Farombi et al, 2007;Zheng et al, 2016). In our study, the absence of oxidative stress at this concentration could be due to the protective role of other organs (i.e.…”

Section: Antioxidant Parametersmentioning

confidence: 51%

Effects of chronic exposure to cadmium and temperature, alone or combined, on the threespine stickleback (Gasterosteus aculeatus): Interest of digestive enzymes as biomarkers

et al. 2018

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The development of predictive, sensitive and reliable biomarkers is of crucial importance for aquatic biomonitoring to assess the effects of chemical substances on aquatic organisms, especially when it comes to combined effects with other stressors (e.g. temperature). The first purpose of the present study was to evaluate the single and combined effects of 90 days of exposure to an environmental cadmium concentration (0.5 μg L) and two water temperatures (16 and 21 °C) on different parameters. These parameters are involved in (i) the antioxidant system (superoxide dismutase activity -SOD- and total glutathione levels -GSH-), (ii) the energy metabolism, i.e. energy reserves (glycogen, lipids, proteins) and digestive enzymes (trypsin, amylase, intestinal alkaline phosphatase -IAP-), and (iii) biometric parameters (weight, length, Fulton's condition factor, and the gonadosomatic index -GSI-) of threespine stickleback (Gasterosteus aculeatus). The second purpose was to determine the interest of the three digestive enzymes as biomarkers in comparison with the other parameters. The higher temperature (21 °C) impacted the anti-oxidant and energy reserve parameters. In liver, GSH levels increased on day 60, while SOD decreased on days 15 and 90, with a significant decrease of protein and lipid energy reserves on day 90. In muscle, the higher temperature decreased SOD activity only on day 90. G. aculeatus biometric parameters were also impacted by the higher temperature, which limited stickleback growth after 90 days of exposure. In female sticklebacks, the GSI peaked on day 60 and decreased sharply on day 90, while the highest values were reached at day 90 in the control groups, suggesting impaired reproduction in sticklebacks raised at 21 °C. These results suggest that 21 °C is an upper-limit temperature for long-term physiological processes in sticklebacks. In contrast, very low-concentration cadmium exposure had no effect on classical biomarkers (energy reserves, antioxidant parameters, biometric parameters). However, digestive enzymes showed an interesting sensitivity to cadmium, which was emphasized by high temperature. The activity of the three digestive enzymes decreased significantly on day 90 when sticklebacks were exposed to cadmium alone, while the decrease was stronger and was recorded earlier (from day 15) when they were exposed to the cadmium-temperature combination. Compared to conventional measurements, digestive enzymes responded rapidly. This could be an important advantage for them to be used as early warning tools to reflect the health status of organisms, particularly for trypsin and IAP activities.

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“…Cd is a potent immunotoxicant, which has been reported to affect the immunocytes and immune organs both in humans and in rodents [ 45 ]. Researches indicated that Cd exposure dramatically enhanced mRNA level of TNF-α via the master inflammatory regulator— NF-κB in the brain, ovary and liver of zebrafis [ 46 , 47 ]. NF-κB is widely distributed in biological organisms that participate in the regulation of multiple genes, inflammatory response and immune response and is essential in priming inflammasome activation for production of cytokines like TNF-α , IL-6 and also of pro- IL-1β [ 48 , 49 ].…”

Section: Discussionmentioning

confidence: 99%

Astragalus Polysaccharide Protect against Cadmium-Induced Cytotoxicity through the MDA5/NF-κB Pathway in Chicken Peripheral Blood Lymphocytes

Xie

et al. 2017

Molecules

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Cadmium (Cd) is a known environmental pollutant that is associated with inflammation, oxidative stress, and cell apoptosis. Astragalus polysaccharide (APS) is a major component of Astragalus membranaceus, a vital qi-reinforcing herb medicine with favorable immuneregulation properties. To study the effect of APS on the inhibition of the cadmium-induced injury of peripheral blood lymphocytes (PBLs) in chickens through the MDA5/NF-κB signaling pathway, PLBs acquired from 15-day-old chickens were divided into control group, Cd group, APS + Cd group, anti-MDA5 mAb + Cd group, BAY 11-7082 (a nuclear factor kappa-light chain-enhancer of activated B cells [NF-κB] inhibitor) +Cd group, APS group, anti-MDA5 mAb group, and BAY 11-7082 group. The transcription levels of melanoma differentiation-associated gene 5 (MDA5), interferon promoter-stimulating factor 1 (IPS-1), NF-κB, and inflammatory factors tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 were measured by quantitative real-time PCR. MDA5 protein expression was measured by western blotting. Levels of malondialdehyde (MDA), glutathione peroxidase (GSH-Px), and superoxide dismutase (SOD) were measured by corresponding antioxidant kit. The morphological change of PBLs was measured by transmission electron microscopy. The results showed that Cd significantly increased the expression of MDA5, IPS-1, NF-κB, and their downstream cytokines, IL-1β and TNF-α, IL-6 in PLBs. In addition, a high level of MDA was observed in the Cd treatment group; the activities of GSH-Px and SOD were significantly lower in the Cd treatment group than those in controls (p < 0.05). Ultrastructural changes of PBLs showed that Cd promoted autophagy, apoptosis, and necrosis in PBLs. However, APS can efficiently improve Cd-induced cell damage by decreasing the activation of the MDA5 signaling pathway. The effect is consistent with that of anti-MDA5 mAb or/and BAY. The results indicated that APS inhibited Cd-induced cytotoxicity through the regulation of MDA5/NF-κB signaling.

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Chronic waterborne zinc and cadmium exposures induced different responses towards oxidative stress in the liver of zebrafish

Cited by 96 publications

References 51 publications

Untitled

Untitled

Effects of chronic exposure to cadmium and temperature, alone or combined, on the threespine stickleback (Gasterosteus aculeatus): Interest of digestive enzymes as biomarkers

Astragalus Polysaccharide Protect against Cadmium-Induced Cytotoxicity through the MDA5/NF-κB Pathway in Chicken Peripheral Blood Lymphocytes

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