Antioxidant, cytotoxic and antimutagenic activities of 7-epi-clusianone obtained from pericarp of Garcinia brasiliensis

Carvalho-Silva, Luciano Bruno de; Oliveira, Maysa do Vale; Gontijo, Vanessa Silva; Oliveira, Williana F.; Derogis, Priscilla Bento Matos Cruz; Stringheta, Paulo César; Nagem, Tânus Jorge; Brigagão, Maı́sa Ribeiro Pereira Lima; Santos, Marcelo H. dos

doi:10.1016/j.foodres.2012.03.003

Cited by 22 publications

(10 citation statements)

References 39 publications

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“…Brine shrimp lethalities of the crude extracts were determined by using the brine shrimp (Artemia salina) lethality assay (Carvalho-silva et al, 2012) which is considered as a useful tool for preliminary assessment of toxicity as a positive correlation exists between brine shrimp lethality and human carcinoma. Shrimp eggs (50 mg), were incubated at 37 ºC in brine solution (38 g sea salt in 1 L of distilled water, pH 7.4), for 48-72 hours to provide large numbers of larvae.…”

Section: Brine Shrimp Lethality Assay (Bsla)mentioning

confidence: 99%

Antibacterial, antifungal, antioxidant, brine shrimp lethality and polyphenolic content of Holarrhena mitis (Vahl) R.Br. ex Roem. & Schult

et al. 2018

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Holarrhena mitis (Vahl) R.Br. ex Roem. & Schult. which is an endemic plant growing mainly in the dry regions of the low-country has been used in the treatment of dysentery in Ayurvedic medicine. During the present study, we tested antibacterial, antifungal, antioxidant activities and brine shrimp lethality, as well as the total phenolic content of the dichloromethane, ethyl acetate and methanol extracts of the bark and leaves of H. mitis. The methanol extract of bark produced a measurable zone of inhibition against two Candida species, namely Candida albicans, Candida krusei among all five tested species and against both dermatophytes, Microsporum gypseum and Tricophyton mentagrophytes. In addition, the methanol extract of bark showed very strong antifungal activity against C. krusei (20 mm), which is very close to that of the positive control ketoconazole (22 mm). Both dicholoromethane extracts of bark and leaves and methanol extracts of leaves showed an activity against both tested strains of Staphylococcus aureus but were negative against Escherichia coli and Salmonella enteritidis. However, the rest of the extracts exerted an active against all the tested bacterial strains. In the brine shrimp cytotoxicity assay, the dichloromethane extracts of both leaves and bark showed lower LC 50 values (27.13 ppm and 9.38 ppm, respectively) than that of positive control, K 2 Cr 2 O 7 , (35.74 ppm) indicating cell toxicity. Compared to the positive control, DL-α-tocopherol (IC 50 : 12.2 ppm), the antioxidant activity of the ethyl acetate and methanol extracts of leaves exhibited comparable activity (IC 50 :16.9 ppm and 29.8 ppm, respectively). Antioxidant activity correlated well with the polyphenol content of methanol and ethyl acetate extracts (473.25 and 138.74 mg (GAE) /g, respectively) of leaves, with respect to gallic acid. These empirical results revealed that methanol extract of bark of H. mitis showed strong antifungal activity as well as very low brine shrimp lethality indicating that it would be a potential nontoxic anti-fungal natural product. The dichloromethane extracts exhibited strong brine shrimp lethality and may contain potential natural anticancer lead compounds. Ethyl acetate extracts of both leaves and bark having significant antibacterial activity, would be source of potential antibacterial lead compounds.

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Section: Brine Shrimp Lethality Assay (Bsla)mentioning

confidence: 99%

Antibacterial, antifungal, antioxidant, brine shrimp lethality and polyphenolic content of Holarrhena mitis (Vahl) R.Br. ex Roem. & Schult

et al. 2018

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“…Some reports state that antioxidants are compounds which can neutralize free radicals and have antimutagenic activity. The phenolic compounds, especially flavonoids and phenolic acids, are antioxidant compounds contained in plants 3 . The phenolic compounds can neutralize free radicals, catalyze the formation of metallic chelate compounds, increase the activation of antioxidant enzymes, and inhibit the oxidation process.…”

Section: Introductionmentioning

confidence: 99%

Determination of Total Phenolic Content, Total Flavonoid Content, and Antimutagenic Activity of Ethanol Extract Nanoparticles of Rhaphidophora Pinnata (L.F) Schott Leaves

Sumaiyah¹,

Masfria²,

Dalimunthe³

2018

RJC

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Phenolic and flavonoid compounds in plants are a natural source of antioxidants which can inhibit the development of several types of diseases in humans, such as cardiovascular diseases and cancer. This study aimed to determine total phenolic content, total flavonoid content, and antimutagenic activity of ethanol extract nanoparticles of Rhaphidophora pinnata (L.f) Schott leaves. The total phenolic content and the total flavonoid content of Rhaphidophora pinnata extract was determined with Folin-Ciocalteu method and aluminum chloride. The antimutagenic activity of the ethanol extract of Rhaphidophora pinnata leaves was evaluated based on the bone marrow micronucleus assay method. The total phenolic content of the ethanol extract of Rhaphidophora pinnata was 49.2 mg gallic acid equivalent/g and the total flavonoid content was 21.8 mg quercetin equivalent/g. The administration of ethanol extract nanoparticles of Rhaphidophora pinnata at a dose of 200, 400, 800 mg/kgBW could decrease the formation of micronucleus in the spinal cord of rats compared to the cyclophosphamide group (P<0.05). The ethanol extract nanoparticles of Rhaphidophora pinnata had antimutagenic activity and dosedependent protection effects against cyclophosphamide -inducer of DNA oxidative damage.

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“…Plants from the genus Rheedia are often used in Brazilian folk medicine to treat disorders, such as constipation, rheumatism, inflammation, and pain [ 5 ]. Rheedia brasiliensis is native to the Amazonian region, planted all across Brazil, and popularly known as bacupari [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Antibacterial Activity of 7-Epiclusianone and Its Novel Copper Metal Complex on Streptococcus spp. Isolated from Bovine Mastitis and Their Cytotoxicity in MAC-T Cells

Barros

Perciano²,

Santos

et al. 2017

Molecules

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Mastitis is an inflammation of mammary gland parenchyma that adversely affects bovine health and dairy production worldwide despite significant efforts to eradicate it. The aim of this work was to characterize the antimicrobial activity of 7-epiclusianone (7-epi), a compound extracted from the Rheedia brasiliensis fruit, its complex with copper against Streptococcus spp. isolated from bovine mastitis, and to assess their cytotoxicity to bovine mammary alveolar cells (MAC-T). The complex 7-epiclusianone-Cu (7-epi-Cu) was an amorphous green solid with optical activity. Its vibrational spectrum in the infrared region showed absorption bands in the high-frequency region, as well as bands that can be attributed to the unconjugated and conjugated stretching of the free ligand. The complex was anhydrous. One of the tested bacterial strains was not sensitive to the compounds, while the other three had MIC values of 7.8 µg mL−1 and minimum bactericidal concentration (MBC) values between 15.6 and 31.3 µg mL−1. These two compounds are bacteriostatic, did not cause damage to the cell wall and, at sub-inhibitory concentrations, did not induce bacterial adhesion. The compounds were not cytotoxic. Based on these results, 7-epi and 7-epi-Cu exhibited desirable antimicrobial properties and could potentially be used in bovine mastitis treatment.

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Antioxidant, cytotoxic and antimutagenic activities of 7-epi-clusianone obtained from pericarp of Garcinia brasiliensis

Cited by 22 publications

References 39 publications

Antibacterial, antifungal, antioxidant, brine shrimp lethality and polyphenolic content of <i>Holarrhena</i> <i>mitis</i> (Vahl) R.Br. ex Roem. & Schult

Antibacterial, antifungal, antioxidant, brine shrimp lethality and polyphenolic content of <i>Holarrhena</i> <i>mitis</i> (Vahl) R.Br. ex Roem. & Schult

Determination of Total Phenolic Content, Total Flavonoid Content, and Antimutagenic Activity of Ethanol Extract Nanoparticles of Rhaphidophora Pinnata (L.F) Schott Leaves

Antibacterial Activity of 7-Epiclusianone and Its Novel Copper Metal Complex on Streptococcus spp. Isolated from Bovine Mastitis and Their Cytotoxicity in MAC-T Cells

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