Antibacterial Activity of 7-Epiclusianone and Its Novel Copper Metal Complex on Streptococcus spp. Isolated from Bovine Mastitis and Their Cytotoxicity in MAC-T Cells

Barros, Mariana de; Perciano, Pedro Griffo; Santos, Marcelo H. dos; Oliveira, Leandro Licursi de; Costa, Éderson D'Martin; Moreira, Maria Aparecida Scatamburlo

doi:10.3390/molecules22050823

Cited by 16 publications

(14 citation statements)

References 57 publications

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“…7- epi -clusianone and its configurational isomers are natural products that have been demonstrated to feature antimicrobial, anti-allergenic, schistosomicidal, and anti-inflammatory effects [30,31,32,33]. Additionally, these compounds have been shown to induce cell death in glioblastoma, as well as lung, melanoma, breast, prostate, renal, cervical, and tongue cancer [34,35,36,37].…”

Section: Introductionmentioning

confidence: 99%

7-epi-Clusianone, a Multi-Targeting Natural Product with Potential Chemotherapeutic, Immune-Modulating, and Anti-Angiogenic Properties

et al. 2019

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Targeted therapies have changed the treatment of cancer, giving new hope to many patients in recent years. The shortcomings of targeted therapies including acquired resistance, limited susceptible patients, high cost, and high toxicities, have led to the necessity of combining these therapies with other targeted or chemotherapeutic treatments. Natural products are uniquely capable of synergizing with targeted and non-targeted anticancer regimens due to their ability to affect multiple cellular pathways simultaneously. Compounds which provide an additive effect to the often combined immune therapies and cytotoxic chemotherapies, are exceedingly rare. These compounds would however provide a strengthening bridge between the two treatment modalities, increasing their effectiveness and improving patient prognoses. In this study, 7-epi-clusianone was investigated for its anticancer properties. While previous studies have suggested clusianone and its conformational isomers, including 7-epi-clusianone, are chemotherapeutic, few cancer types have been demonstrated to exhibit sensitivity to these compounds and little is known about the mechanism. In this study, 7-epi-clusianone was shown to inhibit the growth of 60 cancer cell types and induce significant cell death in 25 cancer cell lines, while simultaneously modulating the immune system, inhibiting angiogenesis, and inhibiting cancer cell invasion, making it a promising lead compound for cancer drug discovery.

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Section: Introductionmentioning

confidence: 99%

7-epi-Clusianone, a Multi-Targeting Natural Product with Potential Chemotherapeutic, Immune-Modulating, and Anti-Angiogenic Properties

et al. 2019

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“…7-epiclusianone (7-epi) ( Figure 2 ) derived from gut-A exhibits a broad spectrum of biological activities ( Ionta et al, 2015 ), including toxicity to Trypanosoma cruzi trypomastigotes ( Alves et al, 1999 ), anti-HIV ( Piccinelli et al, 2005 ), vasodilation ( Cruz et al, 2006 ), anti-anaphylaxis ( Neves et al, 2007 ), antinociception and anti-inflammatory activities ( Santa-Cecília et al, 2011 ), antimicrobial activity against Streptococcus mutans ( Almeida et al, 2008 ) and other Streptococcus spp. ( Barros et al, 2017 ), inhibition of cell reproduction in MDCK and IIN-5 cells ( Vieira et al, 2009 ), and antiproliferation activity against cancer cells ( Almeida et al, 2008 ).…”

Section: Introductionmentioning

confidence: 99%

Synergism of Plant Compound With Traditional Antimicrobials Against Streptococcus spp. Isolated From Bovine Mastitis

et al. 2018

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Mastitis is an inflammation of the mammary gland that causes major losses in the dairy industry. Streptococcus spp. are among the main agents of this disease. Increased resistance to antibiotics is one of the causes of therapeutic failure. Plants, due to their broad chemodiversity, are an interesting source of new molecules with antibacterial activity. Using these compounds along with traditional antibiotics is a possible method for reversing resistance. The objective of this work was to determine the interactions between the activities of guttiferone-A and 7-epiclusianone, two active substances isolated from the fruits of Garcinia brasiliensis, and traditional antibiotics against Streptococcus spp. isolated from bovine mastitis and known to be resistant to them. First, the MIC for the antibiotics and bioactive compounds was determined, followed by their activities, alone and in combination. Then, their cytotoxicity was measured in bovine mammary epithelial cells. Finally, molecular docking simulations were performed to elucidate molecular details of the interactions between β-lactamase and the compounds binding to it (clavulanic acid, ampicillin, 7-epiclusianone, and guttiferone-A). The bacterial isolates were resistant to ampicillin and gentamicin. Both antibiotics showed predominantly synergistic antibacterial activities in combination with guttiferone-A or 7-epiclusianone. These two active substances were not cytotoxic at synergistic concentrations and both showed strong binding to β-lactamase, which may explain the reversal of ampicillin resistance. These substances are promising for the treatment of bovine mastitis.

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“…Hasil penelitiannya menunjukkan bahwa sapi Holstein yang diberi tambahan 20 ppm Cu sulfat mengandung bakteri dan SCC lebih rendah jika dibandingkan dengan sapi tanpa penambahan Cu sulfat. Reyes-Jara et al, (2016) Barros et al (2017) dengan menggunakan senyawa metal complex 7-epiclusianone-copper (7epi-Cu) menunjukkan bahwa senyawa tersebut memiliki sifat antibakterial terhadap Streptococcus spin vitro.…”

Section: Cu Dan Mastitisunclassified

Review : Kejadian mastitis dan kaitannya dengan vitamin dan Trace Mineral Cu, Zn, Se

Yanuartono¹,

Nururrozi²,

Indarjulianto³

et al. 2018

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<p>Mastitis means inflammation of the udder and is a common disease among dairy cows worldwide. It is subdivided into clinical mastitis and subclinical mastitis, both influence milk quality and quantity, and mastitis is therefore of major economic concern for the farmer. The incidence rate of mastitis varies between herds and depends on the exposure to pathogens, environmental and management factors and the immune status of the cow. Mastitis is associated with release of free radicals, increased total oxidant capacity and decreased total antioxidants capacity in milk. Vitamin C, E, , α tocopherol, A, β-karoten and trace mineral (Cu, Zn, and Se) feeding, as an antioxidant, in dairy cows have shown appreciable protective effects by reducing the incidence of mastitis.The purpose of this review is to discuss the role of Vitamins C, E, α tocopherol, A, β-carotene, Cu, Zn, and Se in the incidence of mastitis.</p>

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Antibacterial Activity of 7-Epiclusianone and Its Novel Copper Metal Complex on Streptococcus spp. Isolated from Bovine Mastitis and Their Cytotoxicity in MAC-T Cells

Cited by 16 publications

References 57 publications

7-epi-Clusianone, a Multi-Targeting Natural Product with Potential Chemotherapeutic, Immune-Modulating, and Anti-Angiogenic Properties

7-epi-Clusianone, a Multi-Targeting Natural Product with Potential Chemotherapeutic, Immune-Modulating, and Anti-Angiogenic Properties

Synergism of Plant Compound With Traditional Antimicrobials Against Streptococcus spp. Isolated From Bovine Mastitis

Review : Kejadian mastitis dan kaitannya dengan vitamin dan Trace Mineral Cu, Zn, Se

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