Antioxidant capacity of follicular fluid in relation to follicular size and stage of estrous cycle in buffaloes

El-Shahat, K. H.; Kandil, Mahrousa M. H.

doi:10.1016/j.theriogenology.2011.11.018

Cited by 25 publications

(12 citation statements)

References 26 publications

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“…MDA concentration in LFs did not differ during the late luteal and follicular stages. Similarly, MDA concentrations in SFs did not differ during the early II luteal, late luteal, and follicular stages and these findings were in accordance with those of El-Shahat and Kandil (31). It was expected that LF fluids would have more oxidant activity due to the high metabolic activation.…”

Section: Discussionsupporting

confidence: 82%

Lipid and protein metabolism along with oxidative status of follicular fluidthroughout the estrous cycle in Anatolian water buffalo

Yilmaz

Jaroszewski²,

Bülbül³

et al. 2016

Turk J Vet Anim Sci

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This study was performed to determine the concentrations of creatinine (CRE), high-density lipoprotein (HDL), low-density lipoprotein (LDL), blood urea nitrogen (BUN), nitric oxide (NO), and malondialdehyde (MDA) in follicular fluid (FF) in relation to follicular size and/or stage of the estrous cycle [early I luteal (n = 14), early II luteal (n = 16), late luteal (n = 16), and follicular (n = 16) phases] in Anatolian water buffaloes. The concentrations of CRE (P < 0.001), HDL (P < 0.001), LDL (P < 0.05) BUN (P < 0.001), and NO (P < 0.01) in FF of large follicles (LFs, ≥10 to <20 mm) during the early I luteal phase were lower than those of other stages. In FF of small follicles (SFs, ≥5 to <10 mm), LDL and BUN concentrations were lower during the early I luteal stage (P < 0.05), whereas MDA levels were the highest (P < 0.001). MDA concentrations in SFs were higher than those in LFs during the early I luteal stage (P < 0.001). In conclusion, higher CRE, HDL, LDL, BUN, and NO activity in FF of LFs during luteal stages (early II and late) revealed that these biochemicals may play some roles in follicle development prior to domination and/or ovulation.

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Section: Discussionsupporting

confidence: 82%

Lipid and protein metabolism along with oxidative status of follicular fluidthroughout the estrous cycle in Anatolian water buffalo

Yilmaz

Jaroszewski²,

Bülbül³

et al. 2016

Turk J Vet Anim Sci

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“…The serum level of MDA was significantly low in the GnRH-ant group when compared to the GnRH-a group, but FF level of this was similar in the groups. The animal study has shown that MDA concentrations did not differ between medium and large follicles; however, in the small follicles its concentration was lower at the follicular phase than the luteal phase [50]. The discrepancy of this result between FF and serum could be serum levels reflect entire follicles.…”

Section: Discussionmentioning

confidence: 87%

A comparative study on oxidative and antioxidative markers of serum and follicular fluid in GnRH agonist and antagonist cycles

Çelik

Kumbak

et al. 2012

J Assist Reprod Genet

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Objective To determine whether concentrations of oxidative stress markers of follicular fluid and serum are different in GnRH agonist protocol from GnRH antagonist protocol. Material and method This was a cross-sectional study. Eighty-four women undergoing controlled ovarian stimulation with either GnRH agonist (n039) or GnRH antagonist protocols (n045) for IVF/ICSI treatment were assigned by a physician. Blood was obtained at the time of oocyte retrieval, and follicular fluid (FF) from the mature follicles of each ovary was centrifuged and frozen until analysis. Malondialdehyde (MDA), nitric oxide (NO), protein carbonyl (PC), hydroxyl proline (OH-P), sodium oxide dismutase (SOD), reduced glutathione (GSH), glutathione peroxidase (GSH-Px), adenosine deaminase (ADA) and xanthine oxidase (XO) were assessed in the serum and follicular fluid of each participants. ResultsThe mean serum concentrations of GSH-Px, GSH and MDA were lower in the GnRH antagonist group compared to GnRH agonist group, but mean serum SOD was higher in the GnRH antagonist group. The mean follicular SOD, ADA and NO were higher in GnRH antagonist group than GnRH agonist group. The IVF/ICSI outcomes were similar in both groups. Conclusion(s) GnRH antagonist protocol is associated with increased oxidative stress. The relation of GnRH analogues with oxidative stress and its implication in follicular growth needs to be addressed in further studies.

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“…Both the amount and activity of the three SOD isoforms in follicular fluid are greater in small and medium follicles than in large antral follicles, and these findings have been assessed and verified in different animals [68, 69, 83, 84]. Interestingly, compared with follicular fluid, no changes in SOD have been observed in granulosa cells with regard to the size of the follicles [83].…”

Section: Antioxidants In the Ovariesmentioning

confidence: 98%

“…Singh and Pandey observed that catalase activity in total ovary homogenate was highest in the metestrus phase, declined in the oestrous and pro-oestrous stages, and reached the lowest levels in the diestrus phase [67]. Nevertheless, catalase concentration in follicular fluid is not significantly different among follicles of different sizes [68, 69]. …”

Section: Antioxidants In the Ovariesmentioning

confidence: 99%

“…Studies of ovarian functions have demonstrated that the GSH concentration in follicular fluid in large follicles is significantly higher than that in small follicles during the luteal phase [68]. Luderer et al reported that the GSH content in the ovaries increased from the oestrous to metestrus phase compared with the diestrus to pro-oestrus phase in adult rats [103].…”

Section: Antioxidants In the Ovariesmentioning

confidence: 99%

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The Role of Antioxidant Enzymes in the Ovaries

Wang

Chen

et al. 2017

Oxidative Medicine and Cellular Longevity

127

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Proper physiological function of the ovaries is very important for the entire female reproductive system and overall health. Reactive oxygen species (ROS) are generated as by-products during ovarian physiological metabolism, and antioxidants are indicated as factors that can maintain the balance between ROS production and clearance. A disturbance in this balance can induce pathological consequences in oocyte maturation, ovulation, fertilization, implantation, and embryo development, which can ultimately influence pregnancy outcomes. However, our understanding of the molecular and cellular mechanisms underlying these physiological and pathological processes is lacking. This article presents up-to-date findings regarding the effects of antioxidants on the ovaries. An abundance of evidence has confirmed the various significant roles of these antioxidants in the ovaries. Some animal models are discussed in this review to demonstrate the harmful consequences that result from mutation or depletion of antioxidant genes or genes related to antioxidant synthesis. Disruption of antioxidant systems may lead to pathological consequences in women. Antioxidant supplementation is indicated as a possible strategy for treating reproductive disease and infertility by controlling oxidative stress (OS). To confirm this, further investigations are required and more antioxidant therapy in humans has to been performed. BackgroundReactive oxygen species (ROS) are formed during normal metabolism of oxygen and are produced as by-products of aerobic metabolism. A certain amount of ROS production is necessary for gene expression [1], cell signalling [2,3], and redox homeostasis. Scavenging antioxidant systems are indispensable for maintaining an adequate amount of ROS. The balance between the generation and elimination of ROS is a key factor required for almost every metabolic function in mammals. Maintenance of this balance is an important constitutive process and has a particular influence on cell proliferation, differentiation, apoptosis, and death [4]. When ROS production overwhelms the scavenging ability of antioxidants, oxidative stress (OS) occurs. Unfortunately, disruption of this balance can easily result from either an increase in the concentration of ROS or a decrease in scavenging ability. Excessive ROS levels are harmful to the human body and can result in accumulation of oxidative damage in distinct subcellular compartments that exert very toxic effects on DNA, proteins, and lipids. ROS-mediated damage can ultimately influence physiological functions, such as cell signalling pathways and redox-sensitive signalling pathways, and lead to pathological conditions [5].Regarding the female reproductive system, ROS and antioxidants have been recognized as key factors involved in ovarian physiological metabolism. Many studies have investigated the presence of antioxidants and their transcripts in the female reproductive tract [6][7][8]. Previous studies have reported that the balance between ROS and antioxidants greatly influences th...

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Antioxidant capacity of follicular fluid in relation to follicular size and stage of estrous cycle in buffaloes

Cited by 25 publications

References 26 publications

Lipid and protein metabolism along with oxidative status of follicular fluidthroughout the estrous cycle in Anatolian water buffalo

Lipid and protein metabolism along with oxidative status of follicular fluidthroughout the estrous cycle in Anatolian water buffalo

A comparative study on oxidative and antioxidative markers of serum and follicular fluid in GnRH agonist and antagonist cycles

The Role of Antioxidant Enzymes in the Ovaries

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