Antioxidant, Antiproliferative and Antimicrobial Activities of the Volatile Oil from the Wild Pepper <i>Piper capense</i> Used in Cameroon as a Culinary Spice

Woguem, Verlaine; Maggi, Filippo; Fogang, Hervet Paulain Dongmo; Tapondjou, Léon Azefack; Womeni, Hilaire Macaire; Quassinti, Luana; Bramucci, Massimo; Vitali, Luca Agostino; Petrelli, Dezemona; Lupidi, Giulio; Papa, Fabrizio; Vittori, Sauro; Barboni, Luciano

doi:10.1177/1934578x1300801234

Cited by 23 publications

(18 citation statements)

References 27 publications

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“…The chemical composition of the essential oil of P. capense seed was comparable to those reported in the literature (Woguem et al, 2013, Amvam Zollo et al, 1998, Tchounbougnang et al, 2009). Indeed, previous study showed that essential oil obtained from P. capense seed is mainly constituted by monoterpenes (64.7%) with predominance of hydrocarbons (56.5%).…”

Section: Differences In the Extraction Yields Were Noted Betweensupporting

confidence: 86%

“…Indeed, previous study showed that essential oil obtained from P. capense seed is mainly constituted by monoterpenes (64.7%) with predominance of hydrocarbons (56.5%). Major constituents were monoterpene hydrocarbons: α-pinene (8.9%), sabinene (10.0%), β-pinene (33.2%) and sesquiterpene hydrocarbons: α-caryophyllène (6.3%) and germacrene D (3.8%) (Woguem et al, 2013). Other studies also reported that major volatile compounds of fruits from Western Cameroon were monoterpene hydrocarbons: α-pinene (10.5-14.4%), sabinene (14.7-17.4%), β-pinene (46.8-59.3%) and sesquiterpene hydrocarbons:…”

Section: Differences In the Extraction Yields Were Noted Betweenmentioning

confidence: 99%

“…Evolution of epithelialization percentages in male and female rats during the treatment. Bars represent the mean ± SD of three independent experiments carried out (Woguem et al, 2013)…”

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confidence: 99%

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Chemical composition and antimicrobial activity of essential oils from Aframomum citratum, Aframomum daniellii, Piper capense and Monodora myristica

Dongmo¹,

Tamokou²,

Njateng³

et al. 2019

J. Med. Plants Res.

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This study was initiated to evaluate the chemical composition and in vitro antimicrobial activity of essential oils from four Cameroonian spices and to determine the therapeutic effect of a cream based on essential oil from Aframomum citratum. Essential oils were extracted from seeds by hydrodistillation and analyzed by gas chromatography coupled with mass spectrometry (GC/MS). The broth microdilution method w as used for the minimum inhibitory concentration ( MIC) and minimum microbicidal concentration (MMC) determinations. The therapeutic effect of a cosmetic cream based on essential oil from A. citratum seed (1.25, 2.5 and 5% w/w) was evaluated against dermatosis induced with a Methicillin-Resistant Staphylococcus aureus (MRSA) in rats. The main identified compounds in the essential oils are geraniol for A. citratum; eucalyptol, α-terpineol and geraniol for Aframomum daniellii; β-pinene, germacrene D, trans-β-caryophyllene, α-pinene, naphthalene and sabinene for Piper capense; α-phellandrene, germacradienol and δ-cadinene for Monodora myristica. Essential oil of A. citratum (MIC = 8-4096 µg/ml) was the most active against bacteria and fungi, following in decreasing order by those of A. daniellii, P. capense and M. myristica. The antibacterial activity of the essential oil of A. citratum against MRSA and Escherichia coli S2(1) (MIC = 8 µg/ml) was higher than that of amoxicillin used as reference drug (MIC = 128-256 µg/ml). The combination of essential oils of A. citratum and A. daniellii (1:1) displayed a synergistic effect. The cream based on essential oil of A. citratum (5%) and Baneocin (reference drug) eradicated the dermatosis induced with MRSA in rat after two weeks of treatment. These results indicate that the tested essential oils possess antimicrobial activities which could be a function of either the individual or the additive effects of the identified phytoconstituents.

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Section: Differences In the Extraction Yields Were Noted Betweensupporting

confidence: 86%

Section: Differences In the Extraction Yields Were Noted Betweenmentioning

confidence: 99%

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Chemical composition and antimicrobial activity of essential oils from Aframomum citratum, Aframomum daniellii, Piper capense and Monodora myristica

Dongmo¹,

Tamokou²,

Njateng³

et al. 2019

J. Med. Plants Res.

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“…These oils are basically composed of phenylpropanoids, such as safrole, dillapiole and myristicin, and/or terpenes, such as limonene, β-caryophyllene, spathulenol, (E)-nerolidol, bicyclogermacrene and α-cadinol (Guerrini et al 2009;Santos et al 2001;Maia and Andrade 2009). Investigations of the biological properties of essential oils from this genus have revealed antimicrobial (Oliveira et al 2016), antioxidant (Woguem et al 2013), acaricidal (Araújo et al 2012) and insecticidal activities (Santana et al 2015).…”

Section: Introductionmentioning

confidence: 99%

First record of the chemical composition of essential oil of Piper bellidifolium, Piper durilignum, Piper acutilimbum and Piper consanguineum from the Brazilian Amazon forest

et al. 2018

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Piper bellidifolium, Piper durilignum, Piper acutilimbum and Piper consanguineum are bushes that occur in the Amazon and are morphologically similar. With the aim of analyzing the chemical profile of the volatile constituents of these species, essential oils from the leaves were obtained through steam distillation and analyzed using gas chromatography-flame ionization detection (GC-FID) and gas chromatograph coupled to a mass spectrometer (GC-MS). The chemical analysis enabled the identification of 95 compounds representing 96.3 ± 0.6% of the P. bellidifolium oil, 95.5 ± 0.71% of the P. durilignum oil, 98.0 ± 1.0% of the P. acutilimbum oil and 96.1 ± 2.1% of the P. consanguineum oil. Although sesquiterpenes were the predominant chemical class in the oils of the four species, qualitative and quantitative differences were found in their chemical composition. The major constituents were (E)-nerolidol (20.3 ± 0.4%) in the P. bellidifolium oil, germacrene D (11.1 ± 0.3%) in the P. durilignum oil, and γ-eudesmol in both the P. consanguineum (18.6 ± 0.5%) and P. acutilimbum (7.5 ± 0.4%) oils. Despite their morphological similarity, a principal component analysis (PCA) of the GC-MS data clearly separated the four species according to the chemical profile of the essential oil extracted from their leaves.

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“…However, complexity of the reaction system and high cost of the catalyst prevented the scale-up of the synthetic route at industrial levels [4]. Currently, sabinene is mainly isolated from plants which greatly limits the large-scale production and application of these compounds due to its low content in plant materials [10].…”

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confidence: 99%

Improvement of sabinene tolerance of Escherichia coli using adaptive laboratory evolution and omics technologies

Liu

et al. 2020

Biotechnol Biofuels

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Background: Biosynthesis of sabinene, a bicyclic monoterpene, has been accomplished in engineered microorganisms by introducing heterologous pathways and using renewable sugar as a carbon source. However, the efficiency and titers of this method are limited by the low host tolerance to sabinene (in both eukaryotes and prokaryotes). Results: In this study, Escherichia coli BL21(DE3) was selected as the strain for adaptive laboratory evolution. The strain was evolved by serial passaging in the medium supplemented with gradually increasing concentration of sabinene, and the evolved strain XYF(DE3), which exhibited significant tolerance to sabinene, was obtained. Then, XYF(DE3) was used as the host for sabinene production and an 8.43-fold higher sabinene production was achieved compared with the parental BL21(DE3), reaching 191.76 mg/L. Whole genomes resequencing suggested the XYF(DE3) strain is a hypermutator. A comparative analysis of transcriptomes of XYF(DE3) and BL21(DE3) was carried out to reveal the mechanism underlying the improvement of sabinene tolerance, and 734 up-regulated genes and 857 downregulated genes were identified. We further tested the roles of the identified genes in sabinene tolerance via reverse engineering. The results demonstrated that overexpressions of ybcK gene of the DLP12 family, the inner membrane protein gene ygiZ, and the methylmalonyl-CoA mutase gene scpA could increase sabinene tolerance of BL21(DE3) by 127.7%, 71.1%, and 75.4%, respectively. Furthermore, scanning electron microscopy was applied to monitor cell morphology. Under sabinene stress, the parental BL21(DE3) showed increased cell length, whereas XYF(DE3) showed normal cell morphology. In addition, overexpression of ybcK, ygiZ or scpA could partially rescue cell morphology under sabinene stress and overexpression of ygiZ or scpA could increase sabinene production in BL21(DE3). Conclusions: This study not only obtained a sabinene-tolerant strain for microbial production of sabinene but also revealed potential regulatory mechanisms that are important for sabinene tolerance. In addition, for the first time, ybcK, ygiZ, and scpA were identified to be important for terpene tolerance in E. coli BL21(DE3).

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Antioxidant, Antiproliferative and Antimicrobial Activities of the Volatile Oil from the Wild Pepper Piper capense Used in Cameroon as a Culinary Spice

Cited by 23 publications

References 27 publications

Chemical composition and antimicrobial activity of essential oils from Aframomum citratum, Aframomum daniellii, Piper capense and Monodora myristica

Chemical composition and antimicrobial activity of essential oils from Aframomum citratum, Aframomum daniellii, Piper capense and Monodora myristica

First record of the chemical composition of essential oil of Piper bellidifolium, Piper durilignum, Piper acutilimbum and Piper consanguineum from the Brazilian Amazon forest

Improvement of sabinene tolerance of Escherichia coli using adaptive laboratory evolution and omics technologies

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