Antioxidant, antimicrobial and cytotoxic activities of gold nanoparticles capped with quercetin

Milanezi, Felipe Guzansky; Meireles, Leandra Martins; Scherer, Rodrigo; Oliveira, Jairo Pinto de; Silva, André Romero da; Araújo, Mariceli Lamas de; Endringer, Denise Coutinho; Fronza, Márcio; Guimarães, Marco César Cunegundes

doi:10.1016/j.jsps.2019.07.005

Cited by 86 publications

(34 citation statements)

References 38 publications

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“…The FT-IR spectra of quercetin exhibited its distinguishing characteristic peaks at 3289.58 cm −1 (-OH stretching), 1672.73 cm −1 (C=O stretching), 1521.9 cm −1 (aromatic C=C stretching) and 1212 cm −1 (aromatic C-O stretching) [45,46]. Cholesterol showed its characteristic peaks at 3405.24 cm −1 (-OH stretching), 2866.69 cm −1 (CH 2 symmetric stretching vibration), 1466.54 cm −1 (asymmetric stretching vibration of CH 2 and CH 3 ), 1376.82 cm −1 (CH 2 and CH 3 bending vibration) and 1056.58 cm −1 (C-O bending vibration) [47,48].…”

Section: Ft-ir Studiesmentioning

confidence: 99%

Quercetin Loaded Monolaurate Sugar Esters-Based Niosomes: Sustained Release and Mutual Antioxidant—Hepatoprotective Interplay

et al. 2020

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Flavonoids possess different interesting biological properties, including antibacterial, antiviral, anti-inflammatory and antioxidant activities. However, unfortunately, these molecules present different bottlenecks, such as low aqueous solubility, photo and oxidative degradability, high first-pass effect, poor intestinal absorption and, hence, low systemic bioavailability. A variety of delivery systems have been developed to circumvent these drawbacks, and among them, in this work niosomes have been selected to encapsulate the hepatoprotective natural flavonoid quercetin. The aim of this study was to prepare nanosized quercetin-loaded niosomes, formulated with different monolaurate sugar esters (i.e., sorbitan C12; glucose C12; trehalose C12; sucrose C12) that act as non-ionic surfactants and with cholesterol as stabilizer (1:1 and 2:1 ratio). Niosomes were characterized under the physicochemical, thermal and morphological points of view. Moreover, after the analyses of the in vitro biocompatibility and the drug-release profile, the hepatoprotective activity of the selected niosomes was evaluated in vivo, using the carbon tetrachloride (CCl 4 )-induced hepatotoxicity in rats. Furthermore, the levels of glutathione and glutathione peroxidase (GSH and GPX) were measured. Based on results, the best formulation selected was glucose laurate/cholesterol at molar ratio of 1:1, presenting spherical shape and a particle size (PS) of 161 ± 4.6 nm, with a drug encapsulation efficiency (EE%) as high as 83.6 ± 3.7% and sustained quercetin release. These niosomes showed higher hepatoprotective effect compared to free quercetin in vivo, measuring serum biomarker enzymes (i.e., alanine and aspartate transaminases (ALT and AST)) and serum biochemical parameters (i.e., alkaline phosphatase (ALP) and total proteins), while following the histopathological investigation. This study confirms the ability of quercetin loaded niosomes to reverse CCl 4 intoxication and to carry out an antioxidant effect.Pharmaceutics 2020, 12, 143 2 of 18 and anticancer activities [1][2][3]. These intrinsic properties, together with their biocompatibility and remarkable antioxidant activity, have attracted researchers' attention to explore potential therapeutic applications [4,5].Quercetin (3, 30, 4, 5, 7-pentahydroxyflavone) is probably the most studied bioflavonoid, belonging to the family of flavonols; its therapeutic applications include hepatoprotection [6], prevention of neural cell apoptosis [7] and cancer chemo-prevention and treatment [8].Quercetin acts as strong antioxidant by scavenging free radicals and transition metal ions, thus decreasing the process of lipid peroxidation, which is responsible for the development of many diseases, e.g., cardiovascular and neurodegenerative diseases, as well as liver damage [9][10][11].However, quercetin therapeutic applications present challenges due to (i) low aqueous solubility, (ii) photo and oxidative degradability, (iii) high first-pass effect, (iv) poor intestinal absorption and, hence, low systemic...

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Section: Ft-ir Studiesmentioning

confidence: 99%

Quercetin Loaded Monolaurate Sugar Esters-Based Niosomes: Sustained Release and Mutual Antioxidant—Hepatoprotective Interplay

et al. 2020

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“…Nitric oxide (NO), 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) free radical scavenging assays were performed to evaluate the antioxidant activity of quercetin capped Au nanoparticles. Results demonstrated that capped Au nanoparticles exhibited higher antioxidant activity as compared to quercetin alone [ 102 ].…”

Section: Introductionmentioning

confidence: 99%

“…Both CuO-PEG and CuO-PVP nanoparticles exhibited excellent antioxidant, reducing power and DPPH-free radical scavenging activity. Most interestingly, the antioxidant activities were higher in capped nanoparticles as compared to uncapped ones [ 95 , 102 ]. Similar results were obtained from another study in which ZnO nanoparticles were capped with PEG and PVP showing higher antioxidant activity by capped nanoparticles as compared to uncapped nanoparticles.…”

Section: Introductionmentioning

confidence: 99%

Role of capping agents in the application of nanoparticles in biomedicine and environmental remediation: recent trends and future prospects

et al. 2020

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Capping agents are of utmost importance as stabilizers that inhibit the over-growth of nanoparticles and prevent their aggregation/coagulation in colloidal synthesis. The capping ligands stabilize the interface where nanoparticles interact with their medium of preparation. Specific structural features of nanoparticles are attributed to capping on their surface. These stabilizing agents play a key role in altering the biological activities and environmental perspective. Stearic effects of capping agents adsorbed on the surface of nanoparticles are responsible for such changing physico-chemical and biological characteristics. Firstly, this novel review article introduces few frequently used capping agents in the fabrication of nanoparticles. Next, recent advancements in biomedicine and environmental remediation approaches of capped nanoparticles have been elaborated. Lastly, future directions of the huge impact of capping agents on the biological environment have been summarized.

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“…However, a very slightly negative slope was found which represents very less cytotoxic at high concentration of nanoparticles. The previous study has used very few concentration gold nanoparticles for cytotoxicity analysis [33,34]. We have found that cytotoxicity is very less with a high concentration of nanoparticles.…”

Section: Cytotoxicity Assaysmentioning

confidence: 84%

Facile Synthesis of Triangular and Hexagonal Anionic Gold Nanoparticles and Evaluation of Their Cytotoxicity

et al. 2019

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Comprehension of the shape-dependent properties of gold nanoparticles (AuNPs) could benefit the advancements in cellular uptake efficiency. Spherical AuNPs have generally been used for drug delivery, and recent research has indicated that the cellular uptake of triangular AuNPs was higher than that of spherical ones. Previous reports have also revealed that chemically synthesized AuNPs were cytotoxic. Therefore, we have developed a facile, cost-effective, and environmentally friendly method for synthesizing triangular and hexagonal anionic AuNPs. The zeta potential of the synthesized AuNPs was negative, which indicated that their surface could be easily functionalized with positively charged molecules to upload drugs or biomolecules. Transmission electron microscopy (TEM) images illustrated that the largest particle size of the synthesized quasi-hexagonal AuNPs was 61 nm. The TEM images also illustrated that two types of equilateral-triangular AuNPs were synthesized: One featured sharp and the other rounded corners. The sides of the smallest and largest triangular AuNPs were 23 and 178 nm, respectively. Energy-dispersive X-ray spectra of the green-synthesized AuNPs indicated that they consisted entirely of elemental Au. The cytotoxicity of the green-synthesized AuNPs was evaluated using 3T3-L1 adipocytes. Using cell viability data, we determined that the green-synthesized AuNPs did not exhibit any cytotoxic effects on 3T3-L1 adipocytes.

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Antioxidant, antimicrobial and cytotoxic activities of gold nanoparticles capped with quercetin

Cited by 86 publications

References 38 publications

Quercetin Loaded Monolaurate Sugar Esters-Based Niosomes: Sustained Release and Mutual Antioxidant—Hepatoprotective Interplay

Quercetin Loaded Monolaurate Sugar Esters-Based Niosomes: Sustained Release and Mutual Antioxidant—Hepatoprotective Interplay

Role of capping agents in the application of nanoparticles in biomedicine and environmental remediation: recent trends and future prospects

Facile Synthesis of Triangular and Hexagonal Anionic Gold Nanoparticles and Evaluation of Their Cytotoxicity

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