Antioxidant and tumor cell suppression potential of Premna serratifolia Linn leaf

Abstract

Herbal and natural products have been used in folk medicine for centuries throughout the world. There has been renewed interest in screening higher plants for novel biologically active compounds, particularly those that effectively intervene in human ailments in the field of chronic diseases. The present study has been taken up to evaluate the free radical scavenging activity and tumor cell suppression potential of Premna serratifolia leaf in various in vitro model systems. The methanolic extract of P. serratifolia leaf was obtained by soxhlet extraction method. The superoxide radical scavenging activity, nitric oxide radical, hydroxyl radical, DPPH radical and ABTS radical scavenging activity and lipid peroxidation were determined. The tumor cell suppression cell potential was determined in three different cancer cell lines MCF7 (breast cancer), HepG2 (liver cancer) and A549 (lung cancer) by SRB assay. The study showed that the methanolic extract of P. serratifolia was having free radical scavenging activity against superoxide radical, nitric oxide radical, hydroxyl radical, DPPH radical, ABTS radical and inhibition of lipid peroxidation. The IC50 value showed the efficacy was dose dependent. The test extract showed cytotoxic activity against MCF7, HepG2 and A549 cells. The GI50, TGI and LC50 values were determined against each cell line and compared with standard drug Adriamycin. The present study proved the free radical scavenging activity and tumor cell suppression potential of P. serratifolia leaf in the selective in vitro model systems. The further study has to be carried out in the aspects of isolation of functional molecules of the extract.

Green synthesis of silver nanoparticles using aqueous leaf extract of Premna integrifolia (L.) rich in polyphenols and evaluation of their antioxidant, antibacterial and cytotoxic activity

“…This plant possesses an excellent antioxidant activity [12]. Cytotoxic effects of leaf methanolic extract on liver (HepG2), breast (MCF7) and lung (A549) cancer cell lines have previously been reported [13]. The growth of ehrlich ascites carcinoma (EAC) cell lines [14] was arrested by ethanolic extract of leaf.…”

Effect of aqueous enriched fraction of Premna integrifolia root against cafeteria diet induced obesity in Swiss albino mice

“…[14] Moreover, alkaloids, carbohydrates, amino acids, steroids, flavonoids, glycosides, tannins and phenolic compounds were found in preliminary phytochemical screening of the aqueous enriched fraction of P. integrifolia root (AEFPIR, aqueous extract). [15] P. integrifolia has been reported for its potential actions such as antidiabetic, hypoglycemic and antihyperglycaemic, [16][17][18] anti-inflammatory, [7,19,20] immunomodulatory, [21] cardiac stimulant and cardioprotective, [22,23] analgesic and antibacterial, [24] antimicrobial, [25][26][27] anti-arthritic, [19,28] hepatoprotective, [29][30][31] in-vitro cytotoxic and tumor suppression, [31,32] hypolipidemic or anti-hyperlipidemic and anti-obesity, [3,[33][34][35][36] neuroprotective, [37] antiparasitic, [38] antioxidant, [14,20,29,30,32,39,40] anticoagulation, [41] antitumor, [42] gastroprotective and antiulcer [43] and longevity-promoting. [44] The herbal formulations containing P. integrifolia were also evaluated for their memory enhancing, [45] hypolipidemic, [46] and in-vitro bioactivity effects.…”

Effect of <i>P. serratifolia</i> Leaf Extract on Hematological Parameters and Standarization of Snacks using <i>P. serratifolia</i> Leaf Powder

“…The alcoholic extract of P. serratifolia leaf has hepatoprotective activity against carbon tetrachloride induced hepatotoxicity in rats. According to Selvam et al 9 , the methanolic extract of P. serratifolia leaf has tumor cell suppression potential in 3 different cancer cell lines -breast cancer (MCF7), liver cancer (Hepg2), and lung cancer (A549) than the standard drug adriamycin.…”