Antioxidant and Immunity Activity of Water Extract and Crude Polysaccharide from Ficus carica L. Fruit

Abstract

The antioxidative activities of water extract (WE) and crude hot-water soluble polysaccharide (PS) from Ficus carica L. fruit were investigated using various assays in vitro, including scavenging abilities on DPPH, superoxide and hydroxyl radicals and reducing power. The immunity activities of PS were evaluated using the carbon clearance test and serum hemolysin analysis in mice. In addition, total phenolics and flavonoids contents were also determined. Both WE and PS have notable scavenging activities on DPPH with the EC(50) values of 0.72 and 0.61 mg/ml, respectively. The PS showed higher scavenging activity than WE on superoxide radical (EC(50), 0.95 mg/ml) and hydroxyl anion radical (scavenging rate 43.4% at concentration of 4 mg/ml). The PS (500 mg/kg) also has a significant increase in the clearance rate of carbon particles and serum hemolysin level of normal mice. The results indicate that both WE and PS might be applicable in healthy medicine and food industry.

Nutritional and pharmacological importance of Ficus carica - A review

“…Both water extract and crude hot-water soluble polysaccharide possessed notable scavenging activities on DPPH with the EC 50 values of 0.72 and 0.61 mg/ml, respectively. The crude hot-water soluble polysaccharide showed higher scavenging activity than water extract on superoxide radical (EC 50 , 0.95 mg/ml) and hydroxyl anion radical (scavenging rate 43.4%) at concentration of 4 mg/ml [92]. The free radical scavenging potential of Cyanidin-3-rhamnoglucoside, the major anthocyanin in fresh fig fruits, was evaluated in vitro using several free radical generators.…”

“…The crude hot-water soluble polysaccharide (500 mg/kg) possessed a significant increase in the clearance rate of carbon particles and serum hemolysin level of normal mice [92]. Ficus carica polysaccharides effectively stimulate dendritic cells, partially through the dectin-1/Syk pathway, and promote their maturation, as shown by the up-regulation of CD40, CD80, CD86, and major histocompatibility complex II (MHCII).…”

In vitro antioxidant activity and potential inhibitory action against α-glucosidase of polysaccharides from fruit peel of tea (Camellia sinensis L.)

“…Thus, there has been increasing interest in finding natural, effective, and safe antioxidants, since they may protect the human body from free radicals and retard the progress of many chronic diseases. A review of the literature shows that polysaccharides derived from plants, such as Pteridium aquilinum (Xu et al, 2009), Ficus carica L. (Yang et al, 2009), Lycium barbarum L. (Lin et al, 2009), and Portulaca oleracea L. ) have remarkable antioxidant activity. Meanwhile, the hypoglycemic effect of polysaccharides from plants, particularly the inhibition of postprandial hyperglycemia, has been highlighted recently (Zhu et al, 2013).…”

Ficus Carica and Its Constituents Role in Management of Diseases

“…A study was performed to determine the antioxidant activity, and results of the study revealed that antioxidant activity was found to be very good [25]. Antioxidative activities of water extract and crude hot-water soluble polysaccharide (PS) from fruit was investigated and finding confirmed that both water extract and crude hot-water soluble PS showed prominent scavenging activities with the effective concentration values of 0.72 and 0.61 mg/ml, respectively [26]. Furthermore, The PS also showed a significant increase in the clearance rate of carbon particles and serum hemolysin level of normal mice [26].…”