Antioxidant and free radical-scavenging properties of three flavonoids isolated from the leaves of Rhamnus alaternus L. (Rhamnaceae) : A structure-activity relationship study

Ammar, Rebaï Ben; Bhouri, Wissem; Sghaïer, Mohamed Ben; Boubaker, Jihed; Skandrani, Inès; Neffati, Aïcha; Bouhlel, Inès; Kilani, Soumaya; Mariotte, Anne‐Marie; Chekir‐Ghedira, Leila; Dijoux‐Franca, Marie‐Geneviève; Ghedira, Kamel

doi:10.1016/j.foodchem.2009.02.043

Cited by 123 publications

(84 citation statements)

References 51 publications

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“…Altogether these results revealed that the radical scavenging activities of the tested flavonoids were correlated with the number and position of phenolic hydroxyl groups in the molecules. Bioactivity data confirmed that ortho-dihydroxyl groups in the B-ring and the 2,3-double bond in conjugation with 4-oxo function in the C-ring play a crucial role in radical-scavenging activity in DPPH assay [27][28][29][30][31][32][33][34]. These trends are consistent with less active flavonoids (1-4, 7, 9, 10, 12, and 13) that possess mono-OH substitution in the B-ring.…”

Section: Resultssupporting

confidence: 57%

Fast Identification of Radical Scavengers from Securigera varia by Combining 13C-NMR-Based Dereplication to Bioactivity-Guided Fractionation

et al. 2015

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Securigera varia (Fabaceae) is a common herbaceous perennial plant widely growing in Europe and Asia and purposely established for erosion control, roadside planting, and soil rehabilitation. The aim of this study was to determine the radical scavenging activity of a crude methanol extract of S. varia aerial parts by using the free radical DPPH (1,1-diphenyl-2-picrylhydrazyl) and to rapidly identify the compounds involved in this activity. The crude extract was initially separated in five fractions on Diaion HP20 resin and the most active part was fractionated by Centrifugal Partition Extraction (CPE). Known compounds were directly identified by a 13 C-NMR-based dereplication method. Semi-preparative high performance liquid chromatography purification experiments were further performed to identify unknown or minor active compounds. As a result, one new (13) and twelve known flavonoid glycosides together with three nitropropanoylglucopyranoses were isolated, including astragalin (1) (14), coronillin (16) and coronarian (15). 120 mg of the most active compound isoorientin against the free radical DPPH was recovered by CPE with an HPLC purity of 99%.

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Section: Resultssupporting

confidence: 57%

Fast Identification of Radical Scavengers from Securigera varia by Combining 13C-NMR-Based Dereplication to Bioactivity-Guided Fractionation

et al. 2015

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“…Therefore, the natural products derived from medicinal plants have gained significance in the treatment of cancer (Boopathy and Kathiresan 2010). Recently, there is a growing interest in the use of natural products for anticancer activity against cancer development (Ben Ammar et al 2009;KilaniJaziri et al 2009;Ben Sghaier et al 2011). These include fatty acids, vitamins, minerals, lichens, flavonoids and terpenoids (Chaudhary et al 2009;Iranshahi et al 2009;Ben Sghaier et al 2011;Ranković et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity and genotoxicity of zingiberene on different neuron cell lines in vitro

et al. 2014

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The main objective of this study is to investigate the cytotoxic, genotoxic and antioxidant properties of zingiberene (ZBN) in an in vitro rat brain cell culture study. The cytotoxic effect was determined against the rat neuron and N2a neuroblastoma (N2a-NB) cell lines using the 3,(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, while the antioxidant activity was assessed using the total antioxidant capacity (TAC) and total oxidative stress (TOS) assays. The effects on DNA damage were also evaluated in this study by the single cell gel electrophoresis assay. The results indicated that ZBN has an anti-proliferative activity suppressing the proliferation of N2a-NB cells at concentrations over 50 mg L -1 and neuron cells at concentrations over 150 mg L -1. In addition, ZBN treatments at higher doses (B50 mg L -1 ) led to increases of TOS levels in N2a-NB cell cultures. However 25 mg L -1 of ZBN treatment caused increases of TAC levels in cultured neuron and N2a-NB cell cultures while ZBN at doses of 10-400 mg L -1 did not increase the number of total damage score in both cell lines. This study clearly indicates that ZBN has a significant potential to be used as a natural anticancer agent in cultured N2a-NBs.

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“…Results revealed that the radical scavenging activity of the tested flavonoids was correlated with the number and position of phenolic hydroxyl groups in the molecules [9]. Indeed, isolate 5 with highest DPPH .…”

Section: Radical Scavenging Activity -Dpphmentioning

confidence: 92%

Identification of flavonoid from leaves of gedi (Abelmoschus manihot L.) and its antioxidant activity

Taroreh¹,

Widiyantoro²,

Murdiati³

et al. 2016

AIP Conference Proceedings

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Antioxidant and free radical-scavenging properties of three flavonoids isolated from the leaves of Rhamnus alaternus L. (Rhamnaceae) : A structure-activity relationship study

Cited by 123 publications

References 51 publications

Fast Identification of Radical Scavengers from Securigera varia by Combining 13C-NMR-Based Dereplication to Bioactivity-Guided Fractionation

Fast Identification of Radical Scavengers from Securigera varia by Combining 13C-NMR-Based Dereplication to Bioactivity-Guided Fractionation

Cytotoxicity and genotoxicity of zingiberene on different neuron cell lines in vitro

Identification of flavonoid from leaves of gedi (Abelmoschus manihot L.) and its antioxidant activity

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