Antioxidant and antitumor effects of polysaccharides from the fungus Pleurotus abalonus

Ren, Daoyuan; Jiao, Yong; Yang, Xingbin; Li, Yuan; Guo, Jianjun; Zhao, Yan

doi:10.1016/j.cbi.2015.06.017

Cited by 65 publications

(34 citation statements)

References 42 publications

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“…For example, water-soluble extracts from PS displayed antitumor activity against Sarcoma 180 (Zhuang et al, 1993). Ren et al (2015) demonstrated that polysaccharides from PA exert potent antioxidant and anti-proliferative activities against LoVo cancer cells. Other researchers showed that the polysaccharide-peptide complex from the fruit bodies of PA induced anti-proliferative activities against human hepatoma (HepG2) and human breast cancer cells (MCF-7) (Li et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Antioxidant activity, anti-proliferative activity, and amino acid profiles of ethanolic extracts of edible mushrooms

2016

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ABSTRACT. Biological activities of various mushrooms have recently been discovered, particularly, immunomodulatory and antitumor activities. Herein, three edible mushrooms, Auricularia auricula-judae (AA), Pleurotus abalonus (PA) and Pleurotus sajor-caju (PS) extracted using Soxhlet ethanol extraction were evaluated for their antioxidative, anti-proliferative effects on leukemia cells. Using the Folin-Ciocalteau method and Trolox equivalent antioxidant capacity assay, phenolics and antioxidant activity were found in all sample mushrooms. Additionally, anti-proliferative activity of mushroom extracts against U937 leukemia cells was determined using a viability assay based on mitochondrial activity. PA (0.5 mg/mL) and AA (0.25-0.5 mg/mL) significantly reduced cell viability. Interestingly, PS caused a hormeticlike biphasic dose-response. Low doses (0-0.25 mg/L) of PS promoted cell proliferation up to 140% relative to control, whereas higher doses (0.50 mg/mL) inhibited cell proliferation. Against U937 cells, AA IC 50 was 0.28 ± 0.04 mg/mL, which was lower than PS or PA IC 50 (0.45 ± 0.01 and 0.49 ± 0.001 mg/mL, respectively). Furthermore, lactate dehydrogenase (LDH) leakage conferred cytotoxicity. PS and PA were not toxic to U937 cells at any tested concentration; AA (0.50 mg/mL) showed high LDH levels and caused 50% cytotoxicity. Additionally, UPLC-HRMS data indicated several phytochemicals known to support functional activities as either antioxidant or anti-proliferative. Glutamic acid was uniquely found in ethanolic extracts of AA, and was considered an anti-cancer amino acid with potent anti-proliferative effects on U937 cells. Collectively, all mushroom extracts exhibited antioxidant effects, but their anti-proliferative effects were dose-dependent. Nevertheless, the AA extract, with highest potency, is a promising candidate for future applications.

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Section: Introductionmentioning

confidence: 99%

Antioxidant activity, anti-proliferative activity, and amino acid profiles of ethanolic extracts of edible mushrooms

2016

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“…Another polysaccharide (PAP) has been acquired from P. abalonus. It exhibits antiproliferative properties against human colorectal cancer cells LoVo (Ren et al, 2015). Li and Shah (2014) conducted sulphatation of polysaccharides obtained from P. eryngii cells.…”

Section: Polysaccharidesmentioning

confidence: 99%

Bioactive compounds and medicinal properties of Oyster mushrooms (Pleurotus sp.)

Golak-Siwulska

Kałużewicz

Spiżewski

et al. 2018

Folia Horticulturae

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There are about 40 species in the Pleurotus genus, including those with high economic significance, i.e. P. ostreatus and P. pulmonarius. The fruiting bodies of oyster mushrooms are of high nutritional and health-promoting value. In addition, many species belonging to the Pleurotus genus have been used as sources of substances with documented medicinal properties, such as high-molecular weight bioactive compounds (polysaccharides, peptides and proteins) and low-molecular weight compounds (terpenoids, fatty acid esters and polyphenols). The bioactive substances contained in the mycelium and fruiting bodies of Pleurotus species exhibit immunostimulatory, anti-neoplastic, anti-diabetic, anti-atherosclerotic, anti-inflammatory, antibacterial and anti-oxidative properties. Their multidirectional positive influence on the human organism is the result of interaction of bioactive substances. Extracts from individual Pleurotus species can be used for the production of dietary supplements increasing the organism’s immunity. They are also used for the production of cosmetics. They can be added to functional foods as probiotics, or used as natural preservatives or ingredients of special foodstuffs for patients with specific diseases.

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“…The inhibition ability of WLP on the growth of HepG2 cells was evaluated in vitro using MTT assay (Ren et al, 2015). In this assay, HepG2 cells were seeded in 96-well plates at a density of 1×10 4 per well for 24 h. Afterwards, the medium was aspirated and new serum-free medium containing serial concentrations (0, 1, 3, 5, 7 and 9 mg/mL) of WLP in a volume of 100 µL was added.…”

Section: Cell Viability Assaymentioning

confidence: 99%

“…The intracellular ROS levels of HepG2 cells were detected by fluorescence using DCFH-DA, as described by Ren et al with a little change (Ren et al, 2015). Briefly, the cell suspensions of HepG2 (1×10 4 /well) were pipetted into a 96-well plate and hatched for 24 h to allow attachment.…”

Section: Measurement Of Intracellular Rosmentioning

confidence: 99%

“…Compared with untreated control cells, the intracellular ROS levels of HepG2 cells treated with different concentrations of WLP (1, 5 and 9 mg/ml) for 24 h were signally increased to 130.18%, 214.62% and 242.06%, respectively. The polysaccharides obtained from P. Abalonus and Salvia miltiorrhiza Bunge can augment oxydic stress-induced apoptosis in tumor cells (Ren et al, 2015;Wang et al, 2018). Antioxidant NAC was employed to further determine whether HepG2 apoptosis was related to oxidative stress.…”

Section: Wlp Increase Ros Level Of Hepg2 Cellsmentioning

confidence: 99%

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Inhibition Effect of Walnut Leaf Polysaccharide on HepG2 Cells in vitro

Yang¹,

Xiao²,

Yu³

et al. 2019

American Journal of Biochemistry and Biotechnology

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In this study, the effect of Walnut Leaf Polysaccharides (WLP) on human liver tumor HepG2 cells was investigated. The results showed that WLP had dose-dependent and time-dependent inhibitory effects on HepG2 cell viability. Typical apoptosis characteristics were observed in WLP-treated HepG2 cells through 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolyl-carbocyanine iodide (DAPI) staining and Acridine Orange (AO)/Ethidium Bromide (EB) dual staining. Meanwhile, significant decrease in Mitochondrial Membrane Potential (MMP) and increase in intracellular Reactive Oxygen Species (ROS) levels of WLP-induced HepG2 cells were detected by 5,5′,6,6′-Tetrachloro-1,1′,3,3′tetraethylbenzimidazolcarbocyanine iodide (JC-1) and 2′,7′-Dichlorodihydrofluorescein Diacetate (DCFH-DA) fluorescent probes, respectively. Moreover, cell viability, MMP and ROS level of HepG2 cells were observed after intervention with antioxidant NAC. Thus, we speculated that WLP may induce apoptosis of HepG2 cells by decreasing MMP and increasing intracellular ROS levels. The results suggest that WLP could be developed as a promising natural anticancer active substance in the pharmaceutical and functional food industries.

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Antioxidant and antitumor effects of polysaccharides from the fungus Pleurotus abalonus

Cited by 65 publications

References 42 publications

Antioxidant activity, anti-proliferative activity, and amino acid profiles of ethanolic extracts of edible mushrooms

Antioxidant activity, anti-proliferative activity, and amino acid profiles of ethanolic extracts of edible mushrooms

Bioactive compounds and medicinal properties of Oyster mushrooms (Pleurotus sp.)

Inhibition Effect of Walnut Leaf Polysaccharide on HepG2 Cells in vitro

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