Antioxidant and Anti Aging Assays of <I>Oryza sativa</I> Extracts, Vanillin and Coumaric Acid

Abstract

Ipomoea reniformis Chaos is claimed in Indian traditional medical practice to be useful in the treatment of epilepsy and neurological disorders. In the present study, pretreatment effect of methanolic extract of Ipomoea reniformis on epilepsy and psychosis was evaluated in rodents using standard procedures. Besides evaluating epileptic and behavioral parameters, neurotransmitters such as Gamma-Amino Butyric Acid (GABA) in epilepsy and in psychosis dopamine, noradrenaline and serotonin contents in the rodent brain were estimated. The extract pretreatment reduced maximal electro shock; Isoniazid (INH) and Pentylenetetrazole (PTZ) induced seizures and also significantly inhibited the attenuation of brain GABA levels by INH and PTZ in mice. These results suggested that the observed beneficial effect in epilepsy may be by enhancing the GABAergic system. The test drug also inhibited the apomorphine induced climbing and stereotyped behavior and showed significantly reduced levels of brain dopamine, noradrenaline and serotonin which may be due to blocking of central dopaminergic, noradrenergic and serotonergic pathways or by enhancing the GABAergic system. The results obtained in present study suggest that the title plant possesses antiepileptic and antipsychotic activities in rodents.

Antioxidant Activity of Phyllanthus niruri Extract, Rutin and Quercetin

“…(11)(12)(13)(14) Qualitative Phytochemical Screening Assay The phytochemical assay was conducted on P. niruri extract using modified Fransworth method to qualitatively identified the presence of phytochemicals. (12,(15)(16)(17) Phenol identification: the presence of phenol indicated by green/red/purple/blue or black color. Steroid/triterpenoid identification: the formation of green/blue color indicates the presence of steroid while red/orange sediment indicates the presence of triterpenoid.…”

“…Afterward, the absorbance was read using a microplate reader at 517 nm. (11,12,19,20) The radical scavenging activity was measured with following formula:…”

“…The percentage inhibition of ABTS radical (%) was determined by the ratio of reducing of ABTS absorbance in the presence of the sample relative to the absorbance in the absence of the sample (negative control). (12,18) The median inhibitory concentration (IC 50 ) were also calculated.…”

“…The absorbance value was measured at 593 nm with a microplate reader (Thermo Scientific Multiscan GO, Thermo Fisher Scientific, Ratastie, Finland). (12,18,19) …”

Topical application and oral supplementation of peptides in the improvement of skin viscoelasticity and density

“…Also, di‐ and tripeptides extracted from rice ( Orysza sativa ) have a great potential to be added in cosmetic formulations, once they are well accepted by the skin and act in the metabolic functions of the skin cells, stimulating the synthesis of fibroblasts, collagen (type III and VII), fibronectin, and integrin, which are responsible for cell adhesion, preventing, and improving skin aging …”