Antioxidant activity of sulfated polysaccharide fractions extracted from Laminaria japonica

Evaluation of the Antioxidant Activity of Coreopsis Tinctoria Nuff. and Optimisation of Isolation by Response Surface Methodology

“…-The hydroxyl radical scavenging activity of each extract was measured according to the method described by Wang et al (9)(10)(11) with some modifications. Extract samples were incubated with 2.0 mmol L -1 EDTA-Fe (0.5 mL), 3 % H 2 O 2 (1.0 mL) and 360 mg mL -1 crocus in 4.5 mL sodium phosphate buffer (150.0 mmol L -1 , pH 7.4) for 30 min at 37 °C.…”

Purification, composition analysis and antioxidant activity of the polysaccharides from Dendrobium nobile Lindl.

“…The hydroxyl radicals scavenging activity of the purified polysaccharides were measured according to the method of Wang, Zhang, Zhang, and Li (2008), with some modifications. Different concentrations (0.01-2.0 mg/ml) samples were incubated with 2 mmol/L EDTA-Fe (0.5 ml), 3% H 2 O 2 (1 ml) and 0.36 mg/ml crocus in 4.5 ml sodium phosphate buffer (150 mM, pH 7.4) for 30 min at 37°C and hydroxyl radical was detected by monitoring absorbance at 520 nm.…”

Hypolipidemic effect of fucoidan fromLaminaria japonicain hyperlipidemic rats

“…L. japonica contains alginate, mannitol, laminaran, fucoidan, cellulose, protein, iodine (Podkorytova et al, 2007) and other mineral substances (Zhao et al, 2008), dibutyl phthalate (Namikoshi et al, 2006), etc. Sulfated polysaccharides in seaweed show diverse biological activities of potential medicinal value, such as anticoagulant (Nishino & Nagumo 1992), antitumor (Zhuang et al, 1995), contraceptive, antiviral (Feldman et al, 1999;Ponce et al, 2003), antioxidant (Qi et al, 2005;Tannin-Spitz et al, 2005;Wang et al, 2008), neuroprotective (Jhamandas et al, 2005), and hypolipidemic effects (Shanmugam & Mody, 2000;Veena et al, 2007). Fucoidan polysaccharide sulfuric acid ester (FPS), a group of sulfated heteropolysaccharides, extracted from L. japonica consists mainly of fucose and sulfate, with a smaller amount of arabinose and a trace of rhamnose.…”