Antioxidant Activity of Ethanolic Extract and Fraction of Salak Fruit Seeds ( Salacca Zalacca (Gaertn.) Voss. ) Using DPPH (2,2-Diphenyl-1-Picrylhydrazyl) Method

Abstract

Salak seeds have been developed as a beverage, but there was still a little amount of research that focused on salak seeds. This research was conducted to find out the chemical compounds and the antioxidant activity of ethanolic extract and fraction of salak fruits seeds ( Salacca zalacca (Gaertn.) Voss. ) which have been grown extensively in Sleman Yogyakarta. Extraction was conducted using maceration, followed by fractionation using vacuum liquid chromatography. The identification of the chemical compounds contained in the ethanolic extract and fraction was performed by thin layer chromatography method, while the antioxidant activity was performed by DPPH method. Comparison of antioxidant activity was seen using IC 50 values. The results showed that ethanol extract and fraction contained phenol, flavonoid, and tannin. The largest antioxidant activity was found in F7 with an IC 50 value of 110.16 μg / ml. Keywords: Antioxidant, salak fruit seeds, DPPH ABSTRAKBiji buah salak telah banyak dikembangkan sebagai minuman kopi biji salak, namun penelitian mengenai kandungan serta manfaatnya belum banyak dilakukan. Penelitian ini bertujuan untuk mengetahui kandungan dan aktivitas antioksidan ekstrak etanol serta fraksi biji buah salak pondoh ( Salacca zalacca (Gaertn.) Voss. ) yang tumbuh di Sleman Yogyakarta. Ekstraksi dilakukan dengan metode maserasi yang dilanjutkan dengan fraksinasi menggunakan metode kromatografi cair vakum. Identifikasi senyawa yang terkandung dalam ekstrak dan fraksi kemudian dilakukan melalui metode kromatografi lapis tipis, sedangkan aktivitas antioksidan dilakukan dengan metode DPPH. Perbandingan aktivitas antioksidan dilihat menggunakan nilai IC 50 . Hasil penelitian menunjukkan bahwa ekstrak etanol dan fraksi mengandung fenol, flavonoid, dan tannin. Aktivitas antioksidan terbesar terdapat pada F7 dengan nilai IC 50 sebesar 110,16 µg/ml.

Antioxidants in the extract of balinese salak rind (Salacca amboinensis (Becc) Mogea) as an alternative substance to decrease blood sugar levels among Mus musculus L. with hyperglycemia

“…This study's results align with the results of research conducted by others containing phenols, flavonoids, tannins, and monoterpenoids. Another study also reported that snake fruit skin extract contains flavonoids, saponins, phenols, tannins, steroids/triterpenoids, and alkaloids [11]. The phytochemical content of the ethanolic extract of Salak skin provides various benefits, such as antioxidants or skin lightening.…”

Hepatoprotective Effects of Balinese Grape Extract (Vitis vinifera L.) on Hepar Histology and Reducing Blood Sugar Levels

“…Balinese grapes that have been dried and reduced in particle size are then extracted using 96% ethanol [11]. The extractant was filtered and then thickened using rotary vapour to produce an extract of Balinese grape [12]. BGE were then screened for their phytochemical content.…”

Fractionation of Snake Fruit Seeds Pondoh for Development of Anemia Treatment

“…However, snake peels and seeds become waste and are get ride to the land [2]. The utilization of snake fruit seeds has become necessary because the snake seeds occupy 30% of the whole snake fruit [3].…”

“…Therefore this study aimed to fractionate SFS Pondoh and analyze phytochemical compounds in SFS fractions to minimize secondary metabolites in lipid fraction. The method used to separate secondary metabolites such as phenols, flavonoids, and tannins has 229.27 ± g / mL IC50 for antioxidant activity in SFS flour [3].…”