Antioxidant Activity of Ethanolic Extract and Various Fractions from Green Tea (Camellia sinensis L.) Leaves

Indarti, Karmika; Apriani, Elsa Fitria; Wibowo, Agung Eru; Simanjuntak, Partomuan

doi:10.5530/pj.2019.11.122

Cited by 13 publications

(12 citation statements)

References 17 publications

(18 reference statements)

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“…The mixture was incubated in a dark room for 35 minutes. After that, its absorbance was measured at a wavelength of 515 nm with a UV-Visible spectrophotometer (Indarti et al, 2019). Measurements were made three times.…”

Section: Antioxidant Activity Testmentioning

confidence: 99%

Formulation And Optimization Peel-Off Gel Mask with Polyvinyl Alcohol and Gelatin Based Using Factorial Design from Banana Peel Flour (Musa paradisiaca L) As Antioxidant

2022

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Banana peel (Musa paradisiaca L) contains flavonoid compounds that act as an antioxidant that has the potential to be developed into cosmetic preparations such as peel-off gel masks. This study aims to determine the effect of variations in the concentration of banana peel flour, PVA, and gelatin on the physical properties of peel-off gel masks and to determine their antioxidant activity. This study uses a factorial design of 23 where the factors used are the concentration of banana peel flour, PVA, and gelatin with 2 levels. Based on the results of data analysis, it was found that there was a significant effect of each factor and the interaction between factors on the spreadability and drying time of the preparation (p<0.05). F5 with a concentration ratio of banana peel flour, PVA, and gelatin of 5:12:5 was chosen as the optimum formula and continued to antioxidant activity test compared to F4 which had a ratio of concentrations of banana peel flour, PVA, and gelatin of 10:14:3. The antioxidant activity produced by F5 was better than F4 with IC50 values of 525.41 and 355.64 ppm, respectively. It can be concluded that the optimization of the dosage formula will affect the activity of the active substance.

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Section: Antioxidant Activity Testmentioning

confidence: 99%

Formulation And Optimization Peel-Off Gel Mask with Polyvinyl Alcohol and Gelatin Based Using Factorial Design from Banana Peel Flour (Musa paradisiaca L) As Antioxidant

2022

Self Cite

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“…Antioxidant activity was called 'very strong' category on IC50 when the value is <50 μg/mL, 'strong' when the value is 50-100 μg/mL, 'moderate' when the value is 101-250 μg/mL, 'weak' when the value is 250-500 μg/mL and does not act as an antioxidant if the value is> 500 μg/mL (Table 2). 19 The DPPH method is the most commonly used in testing antioxidant activity. DPPH solution will react with antioxidants to become diphenylpycrylhydrazine.…”

Section: Discussionmentioning

confidence: 99%

Limpasu Pericarpium : an Alternative Source of Antioxidant From Borneo with Sequential Maceration Method

Zamzani

Triadisti

2021

J. prof. medika

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Antioxidants are substances that can slow down the oxidation process of free radicals. Limpasu plant (Baccaurea lanceolata (Miq) Muell. Arg), an indigenous plant of Borneo, is a natural antioxidant source. The purpose of this study was to determine the antioxidant activity of the limpasu pericarpium extract. The extraction of the limpasu pericarpium was done by maceration method using solvents with increasing polarity ranging from n-hexane, ethyl acetate, and methanol. Antioxidant activities of the three extracts were measured by the DPPH and FRAP methods. The IC50 values of n-hexane, ethyl acetate, and methanol extracts, as well as quercetin using the DPPH method were 517,45 µg/mL, 530,64 µg/mL, 10,63 µg/mL and 6,83 µg/mL, respectively. Meanwhile, the IC50 values obtained from FRAP method were 198,96 µg/mL, 190,07 µg/mL, 661,36 µg/mL, and 7,09 µg/mL, respectively. The results revealed that the methanol extract is more potent than other extracts tested for antioxidant activity.

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“…The extract solution was soaked for 48 hours. The filtrate is filtered and the residue is re-maceration for 24 hours [12]. The filtrate was collected and evaporated using a rotary evaporator at a temperature of 65°C to obtain a thick extract.…”

Section: Methodsmentioning

confidence: 99%

Optimization of Green Synthesis of Silver Nanoparticles From Areca Catechu L. Seed Extract With Variations of Silver Nitrate and Extract Concentrations Using Simplex Lattice Design Method

APRIANI¹

2022

FARMACIA

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Silver nanoparticles have been widely used because of their antibacterial properties. Green synthesis of silver nanoparticles was carried out using the ethanolic extract of Areca catechu L. seed because it can act as a bioreductor and stabilizer. This study aims to optimize the formula for silver nanoparticles using the simplex lattice design method with various concentrations of silver nitrate (AgNO 3 ) and extract. The study used 9 formulas to determine the surface plasmon resonance (SPR) of silver nanoparticles. The ethanolic extract of Areca catechu L. seed contains flavonoid compounds, alkaloids, polyphenols, tannins, and saponins which can reduce and stabilize silver nanoparticles. The flavonoid content was 110.849 mg CE/g extract. Optimum formula obtained from the concentration of silver nitrate (AgNO 3 ) 3 mM and 3 mL of extract concentration of 10% w/v. The maximum wavelength of SPR was 420 nm and the absorbance was 1.427, particle size was 122.53 nm, PDI was 0.35, and zeta potential was -21.17 mV. The results of the thermodynamic stability test showed the formation of an irreversible precipitate, a bathochromic and hypochromic shift in the SPR spectrum, and a decrease in the pH value. The optimum formula has quite good particle characteristics but is still not physically stable. RezumatNanoparticulele de argint au fost utilizate pe scară largă datorită proprietăților lor antibacteriene. Sinteza "verde" a nanoparticulelor de argint a fost realizată folosind extractul etanolic de semințe de Areca catechu L., deoarece acesta poate acționa ca bioreductor și stabilizator. Prezentul studiu are ca scop optimizarea formulei nanoparticulelor de argint folosind metoda de proiectare a rețelei simplex cu diferite concentrații de nitrat de argint (AgNO 3 ) și extract etanolic. Studiul a utilizat 9 formule pentru a determina rezonanța plasmonică de suprafață (RPS) a nanoparticulelor de argint. Extractul etanolic de semințe de Areca catechu L. conține flavonoide, alcaloizi, polifenoli, taninuri și saponine care pot reduce și stabiliza nanoparticulele de argint. Conținutul de flavonoide determinat a fost de 110,849 mg CE/g extract. Formula optimă a fost obținută din concentrația de nitrat de argint (AgNO 3 ) 3 mM și 3 ml de extract cu concentrație de 10% p/v. Lungimea de undă maximă a RPS a fost de 420 nm, iar absorbția a fost de 1,427, dimensiunea particulelor a fost de 122,53 nm, indicele de polidispersitate (IPD) a fost de 0,35, iar potențialul zeta a fost de -21,17 mV. Rezultatele testului de stabilitate termodinamică au arătat formarea unui precipitat ireversibil, o schimbare batocromică și hipocromică în spectrul RPS și o scădere a valorii pH-ului. Formula optimă are caracteristici destul de bune ale particulelor, dar nu este încă stabilă din punct de vedere fizic.

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Antioxidant Activity of Ethanolic Extract and Various Fractions from Green Tea (Camellia sinensis L.) Leaves

Cited by 13 publications

References 17 publications

Formulation And Optimization Peel-Off Gel Mask with Polyvinyl Alcohol and Gelatin Based Using Factorial Design from Banana Peel Flour (Musa paradisiaca L) As Antioxidant

Formulation And Optimization Peel-Off Gel Mask with Polyvinyl Alcohol and Gelatin Based Using Factorial Design from Banana Peel Flour (Musa paradisiaca L) As Antioxidant

Limpasu Pericarpium : an Alternative Source of Antioxidant From Borneo with Sequential Maceration Method

Optimization of Green Synthesis of Silver Nanoparticles From Areca Catechu L. Seed Extract With Variations of Silver Nitrate and Extract Concentrations Using Simplex Lattice Design Method

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