Antioxidant activity of egg-yolk protein hydrolysates in a linoleic acid oxidation system

Sakanaka, Senji; Tachibana, Yumi; Ishihara, Noriyuki; Juneja, Lekh Raj

doi:10.1016/j.foodchem.2003.08.014

Cited by 177 publications

(129 citation statements)

References 23 publications

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“…In addition, the protective effect of coconut water could also be linked to its major essential amino acids which play an important role in cell membrane integrity (Sakanaba et al, 2004;Yong et al, 2009). The cryoprotective potential of amino acids in mammalian spermatozoa during freezing stemmed from their ability to form a layer on the spermatozoa surface, and positively charged molecules combined with the phosphate groups of sperm plasma membrane phospholipids has been observed (Kundu et al, 2001;Atessahin et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Effects of coconut (Cocos nucifera) water with or without egg-yolk on viability of cryopreserved buck spermatozoa

et al. 2016

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The effects of semen extenders containing coconut (Cocos nucifera) water alone or combined with egg yolk on viability of spermatozoa during cryopreservation of semen obtained from West African Dwarf (WAD) bucks were studied. Coconut water (5, 10, 15 and 20 ml/100 ml) was diluted with pooled semen samples in study I, while in study II, the pooled semen samples were diluted with egg yolk and coconut water (EYCW) at 5:10, 10:10 and 10:5 in Tris-based extenders. The control consisted of Tris-based extenders plus sodium citrate. The diluted semen samples were cryopreserved for 30 days and evaluated for sperm viability parameters. Following cryopreservation, acrosome reaction and capacitation of spermatozoa were induced in vitro. The results showed that motility was higher (P < 0.05) in coconut water extenders compared to the control and improvement in this parameter was best at 10% coconut water. More spermatozoa cryopreserved with coconut water underwent induced acrosome reaction and capacitation compared to the control and 10% coconut water had the highest values (P < 0.05). The results showed that motility was better preserved in EYCW5:10 and EYCW10:10 extenders (P < 0.05). While more spermatozoa underwent induced acrosome reaction and capacitation with coconut water and egg yolk extenders at different combinations compared to the control (P < 0.05), the highest values of acrosome integrity and membrane integrity were observed in EYCW5: 10 extenders. The results revealed that 10% coconut water and EYCW5:10 gave better improvement of sperm viability parameters. The findings indicated that coconut water extender improved the quality of cryopreserved spermatozoa of WAD bucks.

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Section: Discussionmentioning

confidence: 99%

Effects of coconut (Cocos nucifera) water with or without egg-yolk on viability of cryopreserved buck spermatozoa

et al. 2016

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“…Many attempts have been made to develop chemical or enzymatic modifications that alter the functional characteristics of egg white protein (11,12). Sakanaka et al (13) found that the egg yolk protein hydrolysates, when compared with its original protein or amino acids mixture, showed stronger antioxidant activity in a linoleic acid oxidation system. In addition, a recent study reported the antioxidant activity of peptides produced from crude egg white by pepsin treatment (14).…”

Section: Introductionmentioning

confidence: 99%

Preparation of Egg White Liquid Hydrolysate (ELH) and Its Radical-Scavenging Activity

Noh

Suh

2015

JFN

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In the present study, an optimum protease was selected to hydrolyze the egg white liquid protein for the antioxidant peptides. Alcalase treatment yielded the highest amount of -amino groups (15.27 mg/mL), while the control (no enzymatic hydrolysis) showed the lowest amount of -amino groups (1.53 mg/mL). Alcalase also gave the highest degree of hydrolysis (DH) value (43.2%) and was more efficient for egg white liquid hydrolysis than the other enzymes. The Alcalase hydrolysate had the highest radical-scavenging activity (82.5%) at a concentration of 5.0 mg/mL. The conditions for enzymatic hydrolysis of egg white liquid with Alcalase were selected as substrate : water ratio of 2:1. Five percent Alacalse treatment did not show significant (P＞0.05) increases of DH and -amino nitrogen content after 24 hhydrolysis. Thirty two hour-hydrolysis with 5% Alcalase is sufficient to make antioxidative egg white liquid hydrolysate from egg white liquid. DPPH and ABTS radical-scavenging activities were significantly (P＜0.05) higher after enzymatic digestion. These results suggest that active peptides released from egg-white protein are effective radical-scavengers. Thus, this approach may be useful for the preparation of potent antioxidant products.

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“…CÂMARA et al, (2011) levantaram a hipótese de que o diluente TRIS, formulado com gema de ovo in natura, teria uma significativa capacidade antioxidante, devido às lipoproteínas da gema do ovo, as quais são capazes de prevenir a oxidação dos ácidos graxos poli-insaturados -PUFAs (SAKANAKA et al, 2004). No presente experimento o diluente utilizado também apresenta gema de ovo na formulação, o que talvez possa ter mascarado os resultados, não se detectando diferença significativa entre os grupos quanto à lipoperoxidação.…”

Section: Resultsunclassified

Glutationa Reduzida Na Lipoperoxidação Do Sêmen Equino Criopreservado

Bender¹,

Sampaio²,

Nogueira³

et al. 2015

Enciclopédia Biosfera

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RESUMOOs procedimentos de manipulação do ejaculado podem aumentar o estresse oxidativo sofrido pelos espermatozoides, com consequente redução da sua longevidade. Estudos mostram que o processo de criopreservação induz a lipoperoxidação, o que suscita a realização de pesquisas que estabeleçam estratégias para reduzir os danos causados. O objetivo do experimento foi avaliar o efeito antioxidante da glutationa reduzida adicionada ao sêmen equino pré-congelação, nas concentrações de 3 mM, 5 mM e 7 mM, sobre os níveis de lipoperoxidação após a descongelação. Os ejaculados (n=19) foram congelados em diluente a base de gema de ovo (Botucrio ® ). Foram analisadas as variáveis espermáticas: motilidade, morfologia, viabilidade/status acrossomal, atividade mitocondrial e nível de lipoperoxidação. As médias dos tratamentos foram comparadas pelo teste de LSD, em nível de 5% de significância. Quando comparada a ação das diferentes concentrações de GSH após a descongelação, não foi observado efeito protetor contra a lipoperoxidação, sendo que as concentrações de 5 mM (45,3% vs. 61,6%) e 7 mM (25,3% vs. 61,6%) causaram uma redução significativa da motilidade quando comparadas ao grupo controle, respectivamente. Conclui-se que as concentrações de glutationa reduzida testadas não conferiram proteção antioxidante durante o processo congelação/descongelação do sêmen equino, com as concentrações de 5 mM e 7 mM apresentando efeito deletério sobre a motilidade espermática. PALAVRAS-CHAVE: antioxidante, espermatozoide, estresse oxidativo, ROS

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Antioxidant activity of egg-yolk protein hydrolysates in a linoleic acid oxidation system

Cited by 177 publications

References 23 publications

Effects of coconut (Cocos nucifera) water with or without egg-yolk on viability of cryopreserved buck spermatozoa

Effects of coconut (Cocos nucifera) water with or without egg-yolk on viability of cryopreserved buck spermatozoa

Preparation of Egg White Liquid Hydrolysate (ELH) and Its Radical-Scavenging Activity

Glutationa Reduzida Na Lipoperoxidação Do Sêmen Equino Criopreservado

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