Effects of coconut (Cocos nucifera) water with or without egg-yolk on viability of cryopreserved buck spermatozoa

Abstract

The effects of semen extenders containing coconut (Cocos nucifera) water alone or combined with egg yolk on viability of spermatozoa during cryopreservation of semen obtained from West African Dwarf (WAD) bucks were studied. Coconut water (5, 10, 15 and 20 ml/100 ml) was diluted with pooled semen samples in study I, while in study II, the pooled semen samples were diluted with egg yolk and coconut water (EYCW) at 5:10, 10:10 and 10:5 in Tris-based extenders. The control consisted of Tris-based extenders plus sodium citrate. The diluted semen samples were cryopreserved for 30 days and evaluated for sperm viability parameters. Following cryopreservation, acrosome reaction and capacitation of spermatozoa were induced in vitro. The results showed that motility was higher (P < 0.05) in coconut water extenders compared to the control and improvement in this parameter was best at 10% coconut water. More spermatozoa cryopreserved with coconut water underwent induced acrosome reaction and capacitation compared to the control and 10% coconut water had the highest values (P < 0.05). The results showed that motility was better preserved in EYCW5:10 and EYCW10:10 extenders (P < 0.05). While more spermatozoa underwent induced acrosome reaction and capacitation with coconut water and egg yolk extenders at different combinations compared to the control (P < 0.05), the highest values of acrosome integrity and membrane integrity were observed in EYCW5: 10 extenders. The results revealed that 10% coconut water and EYCW5:10 gave better improvement of sperm viability parameters. The findings indicated that coconut water extender improved the quality of cryopreserved spermatozoa of WAD bucks.

Effect of Different Egg Yolk Levels in Tris Extender With and Without Aloe Vera and Onion Crude Extracts on Surti Buck Semen at Refrigerated Temperature

“…Moreover, postchilled abnormal sperm count was consistently higher at all the concentrations of egg yolk (5-20%) with onion (EY5+O, EY10+O, EY20+O) followed by Aloe vera (EY5+A, EY10+A, EY20+A) at both 24 and 48 h of storage in present experiment, while the abnormalities were lowest without any additive (EY5, EY10, EY20) groups. Similarly, higher abnormal sperm percentage after Aloe vera treatment in West African Dwarf bucks (Olugbenga et al, 2011), and the non-significant difference between different groups (Daramola et al, 2016a) has been documented, while contradictory findings were reported in other studies (Khalifa and Abdel-Hafez, 2013;Daramola et al, 2016b). Non-significantly higher sperm abnormalities were observed in EY0 than EY5, EY10 and EY20 groups in the present study.…”

A diluent containing coconut water, fructose, and chicken egg yolk increases rooster sperm quality at 5°C

“…Fructose is added to the diluent because the sperm plasma contains a variety of specific organic compounds, one of which is fructose. Egg yolk is a good diluent because it is affordable and accessible, has good energy source, and contains nearly the same physical and biochemical elements as sperm [17].…”

Coconut water-based extender for seminal preservation in small ruminants: A meta-analysis study

“…In addition to having water, sugars, proteins, salts, and vitamins in its composition, coconut water is easy to prepare and has a low cost (Carvalho et al, 2006;Rondon et al, 2008). Moreover, improvements in sperm cells integrity and viability in small ruminants have already been reported with the use coconut water (Cavalcante et al, 2014;Daramola et al, 2016;Brito et al, 2019).…”

“…In seminal plasma, sperms have a limited survival time, thus the necessity of using diluents and cryoprotectants that can maintain sperm quality during the execution of preservation techniques and storage at low temperatures, providing an adequate environment for survival of these gametes and, consequently prolonging sperm viability (Daramola et al, 2016).…”