A diluent containing coconut water, fructose, and chicken egg yolk increases rooster sperm quality at 5°C

Abstract

Aim: The present study was conducted to evaluate the quality of rooster sperm at 5°C after treatment with a diluent containing coconut water, fructose, and chicken egg yolk and stored the semen sample at 5°C. Materials and Methods: Ten semen samples from 10 healthy roosters were subjected to four different treatments. For the treatments, 0.2 ml fresh semen with a sperm concentration of 5.2X109 cell/ml was mixed with T0 (no diluent), T1 (0.34 ml coconut water and 6 μl fructose), T2 (0.274 ml coconut water, 0.12 ml egg yolk, and 6 μl fructose), and T3 (0.34 ml egg yolk and 6 μl fructose) solutions. Each treated solution was stored at 5°C and evaluated both macroscopically and microscopically. Macroscopically, semen volume, pH, and sperm concentration were evaluated. The microscopic sperm characteristics examined included total motility (i.e., rapid, medium, or slow), progressive and non-progressive motility, viability, and spermatozoa abnormalities noted at different storage times. The results showed that spermatozoa motility was under 40%. Results: The results indicated that sperm viability significantly affected (p<0.05). The highest mean value of sperm viability on day 7 of storage was found after treatment with the T2 solution (46.100±0.5677%). Similarly, spermatozoa abnormalities were significantly lower after treatment with the T2 solution (6.680±1.702%). Conclusion: The addition of a diluent containing coconut water, egg yolk, and fructose helped in the better preservation spermatozoa motility, as well as viability for up to 7 days when the semen samples were stored at 5°C.

Pengaruh penambahan ekstrak bawang merah dalam pengencer Ringer’s Dextrose terhadap kualitas sperma Gallus domesticus pada suhu 5°C

“…This result was in accordance with that the pH of chicken semen was between 7.2-7.6 (Iswati et al, 2018). Massa motility was obtained 3+ which was indicated that it was in accordance with Rochmi & Sofyan (2019) who stated that the excellent mass motility was 3+ and marked by cloud-like huddling sperm. Individual motility and viability produced were greater at 85% and 94.17 ± 1.47% compared to Sun et al, (2019) at 67.84%, 71.17% and (Shanmugam et al, 2014) at 46.25 %, 93.01 ± 2.39%.…”

“…Gallus domesticus has an economic value due to its distinctive taste of meat and egg. Therefore, Indonesians put so much interest and have high demand towards it (Rochmi & Sofyan, 2019). Some advantages of the Gallus domesticus breeding compared to purebreed chicken are including the fact that it is more resistance to the disease, has high adaptability to the environment, as well as its breeding is relatively easier.…”

“…The use of lactate ringers diluent was able to maintain semen quality for 32 hours in cold temperatures (Iswati et al, 2018). The chicken's spermatozoa could be stored effectively for 7 days at 5 o C (Rochmi & Sofyan, 2019). The temperature and storage time of semen in the diluent affected the quality of the semen and its motility.…”

Spermatozoa Quality of Kintamani Dogs in Coconut Water-Egg Yolk Diluent with Addition of Moringa Leaves and Carrot Extract

“…The young coconut water diluent with egg yolk contains a nutritional source for spermatozoa metabolism and isotonic properties to semen. It acts as a buffer, protects spermatozoa in the cooling process, and is not toxic (Rochmi and Sofyan 2019;Puja et al 2018). Furthermore, the optimal ratio of young coconut water and egg yolk is 80:20% (Apriliana et al 2021;Priharyanthi et al 2021).…”

The Effect of the Combination of Glucose Concentration with the Type of Extenders on the Quality of Native Rooster Spermatozoa during Storage

“…According to Qiu, Li, Xie, Li, Dong, Sun, Gao & Tan (2016), spermatozoa metabolize glucose during long-term liquid storage of semen, and this is important not only in the goat but also in other species. Previous researchers have proven the effectiveness of the combination of extenders with carbohydrates on the quality of poultry spermatozoa, for example, Rochmi & Sofyan (2019) found a diluent containing a mixture of coconut water, egg yolk, and fructose can be added to rooster sperm to increase spermatozoa motility and viability for up to 7 days when the cement samples are stored at 5 ° C.…”