Antioxidant Activity and Metabolomic Analysis of Cagaitas (Eugenia dysenterica) using Paper Spray Mass Spectrometry

Abstract

Cagaita is a fruit from Brazilian cerrado, belongs to Myrtaceae family, and has important economic value. This work aimed to determine the total antioxidant capacity (extractable and nonextractable fractions) by different methods and to evaluate the use of paper spray mass spectrometry to obtain fingerprints of cagaita from different regions with the aid of principal components analysis. Cagaitas had higher antioxidant activity than those found in other fruits mentioned in literature, and the non-extractable fraction was 18.90 to 21.05% of the antioxidant capacity. The analysis of paper spray mass spectrometry in positive and negative ionization modes identified several substances, including organic acids, sugars, amino acids and several other classes of phenolic compounds. Analysis of the main components of cagaita samples permitted discrimination of the major constituents such as sugars and different kinds of phenolic compounds. Thus, this study demonstrated that paper spray mass spectrometry is a simple and ultrafast method with minimum sample preparation that allows the analysis of the chemical profile of cagaita.

Influence of thermal processing on the characteristics and chemical profile of ora-pro-nobis by PS/MS paper spray

“…The analysis of the chemical profile of the samples was performed using an LCQ Fleet mass spectrometer (Thermo Scientific, San José, CA, USA) equipped with a source of ionization by paper spray. The analysis was performed in triplicate in positive and negative ionization modes, according to Silva et al (2019).…”

Perfil químico da farinha extrusada de sorgo do genótipo BRS 305 por paper spray

“…A Figura 1 mostra o espectro gerado a partir da amostra de farinha extrusada de sorgo no modo negativo de ionização. (Silva et al, 2019;Campelo et al, 2020). Os flavonoides são metabólitos secundários de plantas e o maior grupo de fenólicos naturais presentes em vegetais.…”

“…Para identificação dos compostos em análise foi realizada uma comparação entre as razões massa/carga obtidas no estudo com as encontradas na literatura, mediante fragmentação por espectrometria de massa sequencial. A energia de colisão utilizada para fragmentar os compostos variou de 15 a 40 V e os espectros de massas obtidos foram processados no software Xcalibur (Silva et al, 2019;Ramos et al, 2020).…”

“…Neste cenário, a espectrometria de massas com ionização em paper spray tem sido utilizada na avaliação e controle de qualidade de várias matrizes alimentares de origem vegetal, tais como grumixama (Ramos et al, 2020), açaí (Minighin et al, 2020), gengibre , azeite de oliva (Mazzotti et al, 2013), cagaita (Silva et al, 2019), farinha de banana (Silva et al, 2020), cacau (Oliveira-Júnior et al, 2020), nêspera (Silva et al, 2020b), chá (Deng & Yang, 2013), café (Garrett Research, Society and Development, v. 10, n. 1, e40710111414, 2021 (CC BY 4.0) | ISSN 2525-3409 | DOI: http://dx.doi.org/10.33448/rsd-v10i1.11414 et al, 2013) e pera-do-cerrado (Mariano et al, 2020), por apresentar alta precisão, sensibilidade e possuir baixo custo de operação.…”

Bioactive activities and chemical profile characterization using paper spray mass spectrometry of extracts of Eriobotrya japonica Lindl. leaves

“…However, several stages of sample preparation and long analysis times have been reported as limitations of these techniques. In this sense, ambient ionization techniques such as MS with paper spray (PS) ionization have been shown to be efficient in overcoming these limitations, because they enable fingerprints to be obtained from several complex matrices using ultrafast analyses and low‐cost analytical methods, and without generating chemical residue 8–11 2,4 …”