Antinociceptive activity of Amazonian Copaiba oils

Gomes, Niele M.; Rezende, Cláudia M.; Fontes, Silvia Paredes; Matheus, Maria Eline; Fernandes, Patrícia Dias

doi:10.1016/j.jep.2006.08.018

Cited by 151 publications

(121 citation statements)

References 22 publications

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“…Num estudo conduzido por Fernandes et al (1992) com Copaifera cearensis, camundongos foram expostos a doses de 1250, 2500, 3700 e 5000 mg/kg pc de óleo-resina (dez animais de ambos os sexos/grupo), e a DL 50 estimada em 3090 mg/kg pc. Gomes et al (2007) estimaram a DL 50 do óleo resina de Copaifera reticulata e Copaifera multijuga em camundongos em 3900 mg/kg pc e 4300 mg/kg pc, respectivamente.…”

Section: Resultsunclassified

“…A dose inicial selecionada para o estudo foi de 300 mg/kg pc, definida a partir de dados de estudos prévios com outras espécies desta planta (Ribeiro et al, 2007;Gomes et al, 2007). A próxima dose testada foi selecionada dependendo da mortalidade observada nas primeiras 24 h de exposição.…”

Section: Methodsunclassified

“…O seu uso tem sido indicado, entre outros, pela sua ação antiinflamatória (Carvalho et al, 2005;Veiga Júnior et al, 2007), antitumoral (Lima et al, 2003), antimicrobiana (Santos et al, 2008;Tincusi et al, 2002) e atividade antinociceptiva (Gomes et al, 2007). Apesar da existência de estudos da ação terapêutica do óleo-resina de copaíba, os estudos de toxicidade deste produto são escassos na literatura científica; alguns destes estudos obtiveram resultados contraditórios.…”

Section: Introductionunclassified

“…Segundo Tisserand & Balancs (1995) apud Cavalini et al, 2005 não há relatos de toxicidade oral, cutânea ou fotossensibilidade devido à administração do óleo-resina de copaíba. A administração de 500 mg/kg peso corpóreo do óleo-resina (Copaifera multijuga Hayne e Copaifera reticulata Ducke, Fabaceae) em camundongos por via oral não foi relacionada a alterações comportamentais, perda dos reflexos a luz, aumento ou diminuição da ingestão de água e ração e lesões ou hemorragias no estômago (Gomes et al, 2007). Por outro lado, Souza et al (2000) observaram que a administração de 0,4 mL do óleo-resina pela via transdiafragmática ocasionou diarréia, piloereção e hemorragia nos animais tratados.…”

Section: Introductionunclassified

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Avaliação da toxicidade aguda e potencial neurotóxico do óleo-resina de copaíba (Copaifera reticulata Ducke, Fabaceae)

Sachetti¹,

Fascineli²,

Sampaio³

et al. 2009

Rev. bras. farmacogn.

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RESUMO:O óleo-resina de copaíba obtido do gênero Copaifera L., Fabaceae, é largamente utilizado na medicina popular como antiinflamatório, antimicrobiano e antitumoral. Porém, informações sobre seu potencial tóxico são escassos na literatura. O objetivo deste estudo foi estabelecer a toxicidade oral aguda e os possíveis efeitos neurotóxicos relacionados à ingestão do óleo-resina de Copaifera reticulata Ducke, Fabaceae, em ratas Wistar. O estudo foi conduzido com quinze ratas nulíparas distribuídas nos grupos de doses 300 e 2000 mg/kg pc de óleo-resina administrado por gavagem. Os resultados obtidos mostraram que nestas doses não houve sinais clínicos de toxicidade ou neurotoxicidade, alteração no consumo de ração ou alteração no peso corpóreo. A dose letal aguda foi estimada como maior que 2000 mg/kg pc e classificada como categoria 5, segundo o Guia OECD 423. Estes resultados indicam que existe uma relativa margem de segurança para o uso do óleo-resina de copaíba como agente terapêutico, embora estudos toxicológicos adicionais sejam ainda necessários, principalmente com a administração repetida de baixas doses.Unitermos: copaíba, ratos, toxicidade aguda, neurotoxicidade.ABSTRACT: "Assessment of the neurotoxic potential and acute toxicity of copaiba". Copaiba oil-resin obtained from Copaifera L. genus, Fabaceae, is largely used in popular medicine as antinflammatory, antimicrobial and antitumoral. Information concerning the potential toxicity of this oil is limited in the literature. The goal of this study was to investigate the acute toxicity and the possible neurotoxic effects related to the ingestion of Copaifera reticulata Ducke, Fabaceae, oilresin using female Wistar rats. Fifteen nulliparous rats were used and distributed in the experimental groups orally exposed to doses of 300 e 2000 mg/kg bw of oil-resin (gavage). No overt clinical signs of toxicity or neurotoxicity, alteration of food consumption or body weight were observed in the animals at the tested doses. The lethal oral toxicity was estimated to be higher than 2000 mg/ kg bw, classified as category 5 according to OECD Guide 423. These results indicate that there is a certain safety margin associated with the use of copaiba as therapeutic agent, although additional toxicological studies are still necessary, mainly using repeated low doses.

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Section: Resultsunclassified

Section: Methodsunclassified

Section: Introductionunclassified

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Avaliação da toxicidade aguda e potencial neurotóxico do óleo-resina de copaíba (Copaifera reticulata Ducke, Fabaceae)

Sachetti¹,

Fascineli²,

Sampaio³

et al. 2009

Rev. bras. farmacogn.

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“…antimicrobial and wound healing activities [14][15][16][17][18]. Most studies of gender Copaifera use the oleoresin from the bark, due to its application in folk medicine; however, there are few studies using the aerial parts of the tree [19].…”

Section: Introductionmentioning

confidence: 99%

Hydroalcoholic Extract from Copaifera Langsdorffii Has Skin Wound Healing Activity in Rats

Gushiken

Hussni

Bastos

et al. 2017

IJCAM

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The loss of skin integrity causes wounds, and in response, the organism promotes the healing process, which is divided into three phases: inflammatory, proliferative and remodeling. Several plant species have been tested in healing experimental wounds due to popular application. Among the species used in skin wound healing related in popular knowledge is Copaifera langsdorffii. By this way, we aim to analyze wound healing changes in rats with skin lesions after treatment with hydroalcoholic extract from Copaifera langsdorffii leaves. Male Wistar rats were submitted to excisional injury with a 2 cm diameter in the dorsal region. They were divided into groups (n=6) with daily treatments of hydroalcoholic extract at 1; 5 and 10% concentrations or a negative control (saline) during 3, 7 and 21 days. The lesions were analyzed and measured daily. Blood was collected for toxicity analysis, and dorsal lesions were collected for histological analysis and measurements of cellularity in normal skin, border and center of the lesion. The toxicity data did not show any significant differences between the groups. Microscopic analysis showed an increase in total cell number at the edges of the extract treated groups after 3 days. Macroscopically, the groups treated with 5% and 10% extract demonstrated better wound retraction compared with saline treatment. In summary, the groups treated with hydroalcoholic extract of Copaifera langsdorffii showed the efficacy of the plant as treatment of skin wound healing.

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Development and validation of a rapid RP‐HPLC method for analysis of (−)‐copalic acid in copaíba oleoresin

Souza

Moreira

Borges

et al. 2012

Biomedical Chromatography

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The Copaifera species (Leguminoseae) are popularly known as 'copaíba' or 'copaíva' and are grown in the states of Amazonas, Pará and Ceará in northern Brazil. The oleoresins obtained from these species have been extensively used owing to their pharmacological potential and their application in cosmetic and pharmaceutical preparations. In the present study, the development and validation of a novel, rapid and efficient RP-HPLC methodology for the analysis of the diterpene (-)-copalic acid (CA), pointed out as the only chemical marker of the Copaifera genus, are described. The regression equation (Y = 26,707x - 29,498) was obtained with good linearity (r(2) = 0.9993) and the limits of quantification and detection were 9.182 and 3.032 µg/mL, respectively. The precision and the accuracy of the method were adequate (lower than 4%). Finally, the validation parameters evaluated were satisfactorily met, so the developed method represents a suitable tool for application in the quality control of such natural products. Further studies aiming to develop analytical methodologies for each Copaifera species using a more representative number of chemical markers should be performed.

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Antinociceptive activity of Amazonian Copaiba oils

Cited by 151 publications

References 22 publications

Avaliação da toxicidade aguda e potencial neurotóxico do óleo-resina de copaíba (Copaifera reticulata Ducke, Fabaceae)

Avaliação da toxicidade aguda e potencial neurotóxico do óleo-resina de copaíba (Copaifera reticulata Ducke, Fabaceae)

Hydroalcoholic Extract from Copaifera Langsdorffii Has Skin Wound Healing Activity in Rats

Development and validation of a rapid RP‐HPLC method for analysis of (−)‐copalic acid in copaíba oleoresin

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