Antimutagenicity Screening of Extracts from Medicinal and Edible Plants against N-Methyl-N-nitrosourea by the Ames Assay

Tatsuzaki, Jin; Yang, Jinwei; Kojo, Yukiko; Mine, Yusuke; Ishikawa, Satoko; Mochizuki, Masahito; Inami, Keiko

doi:10.3123/jemsge.2014.007

Cited by 4 publications

(3 citation statements)

References 68 publications

(78 reference statements)

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“…Not only that LAURESH ® is non-mutagenic, a study conducted on laurel revealed that 3-kaempferyl p-coumarate isolated from laurel leaves showed anti-mutagenic activity against a known dietary carcinogen, 3-amino-1-methyl-5H-pyrido[4,3-b]indole (Samejima et al, 1998). However, in another study on anti-mutagenic activity, aqueous laurel extract was not effective against another carcinogen, N-methyl-N-nitrourea (Tatsuzaki et al, 2014). On the other hand, the ability of LAURESH ® in eliciting acute toxicity was evaluated using single dose acute oral toxicity study conducted on five male and five female mice allocated to each of the LAURESH ® and control group.…”

Section: Discussionmentioning

confidence: 99%

Safety studies of LAURESH® a standardized Laurus nobilis leaf extract

Wong¹,

Kobayashi²,

Yang³

2023

Fundam. Toxicol. Sci.

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The erroneous assumption that herbal products is generally safe for consumption, is a major factor leading to the increased of herb-induced liver injury (HILI). Even though Laurus nobilis or laurel is a commonly used spice, the safety aspect for its consumption is under-studied. To bridge this gap of knowledge, the mutagenicity, acute toxicity, and subacute toxicity of LAURESH ® , which is a standardized laurel leaf extract were evaluated. Mutagenicity study using two S. typhimurium strains, TA100 and TA98 indicated that LAURESH ® does not cause base substitution and frameshift mutation, thus suggesting that LAURESH ® is non-mutagenic. While acute oral toxicity on mice established the LD 50 at no less than 2,000 mg/kg of body weight, and a 28-day subacute toxicity test on rat revealed the NOAEL to be 1,000 mg/kg/day. Furthermore, blood chemistry, urinalysis, necropsy, and histopathological data from subacute toxicity study on rats does not show adverse event that could be attributed to LAURESH ® , thus indicating that LAURESH ® is unlikely to cause HILI. Taken together, the findings from this study and previous clinical study on LAURESH ® , in combination with the historic use of laurel and previous toxicity studies conducted on laurel leaves extract, strongly suggest that LAURESH ® is safe for human consumption.

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Section: Discussionmentioning

confidence: 99%

Safety studies of LAURESH® a standardized Laurus nobilis leaf extract

Wong¹,

Kobayashi²,

Yang³

2023

Fundam. Toxicol. Sci.

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“…In our previous study, a powdered ethanolic extract of G. aspera root decreased MNU-induced mutagenicity in a preliminary antimutagenic screen using the Ames assay [30]. The aim of this study was to identify the antimutagenic components of the powdered ethanolic extract of G. aspera root.…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of antimutagenic components of Glycyrrhiza aspera against N-methyl-N-nitrosourea

Inami

Mine

Tatsuzaki³

et al. 2017

Genes and Environ

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BackgroundA powdered ethanolic extract of Glycyrrhiza aspera root exhibits antimutagenic activity against N-methyl-N-nitrosourea (MNU) based on the Ames assay with Salmonella typhimurium TA1535. The aim of this study was to identify the antimutagenic components of the powdered ethanolic extract of G. aspera root.ResultsThe powdered ethanolic extract of G. aspera root was sequentially suspended in n-hexane, carbon tetrachloride, dichloromethane, ethyl acetate, and ethanol, and each solvent soluble fraction and the residue were assayed for antimutagenic activity against MNU in S. typhimurium TA1535. The dichloromethane soluble fraction exhibited the highest antimutagenicity and was fractionated several times by silica gel chromatography. The fraction with the highest antimutagenic activity was further purified using HPLC, and the fractions were assayed for antimutagenicity against MNU in S. typhimurium TA1535. Finally, five components with antimutagenic activity against MNU were identified as glyurallin A, glyasperin B, licoricidin, 1-methoxyphaseollin, and licoisoflavone B.ConclusionsThe five components were demonstrated to possess an antigenotoxic effect against carcinogenic MNU for the first time. It is important to prevent DNA damage by N-nitrosamines for cancer chemoprevention.Electronic supplementary materialThe online version of this article (doi:10.1186/s41021-016-0068-2) contains supplementary material, which is available to authorized users.

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“…Antimutagenic agents are known to counteract the effects of mutagenic agents, and there is a high demand to identify such compounds ( Sloczynska et al , 2014 ). According to some authors ( De Flora and Ferguson, 2005 ; Steward and Brown, 2013 ; Tatsuzaki et al , 2014 ), the daily consumption of antimutagenic agents could prevent cancer and genetic diseases in humans. For example, Varanda et al (1999) have shown an antimutagenic effect exerted by an ethanolic extract of propolis on Salmonella typhimurium (TA102, TA100 and TA98).…”

Section: Introductionmentioning

confidence: 99%

Antigenotoxicity and antimutagenicity of ethanolic extracts of Brazilian green propolis and its main botanical source determined by the Allium cepa test system

et al. 2016

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Brazilian green propolis is a resinous substance prepared by bees from parts of the plant Baccharis dracunculifolia. As it possess several biological properties, this work assessed the cytotoxic/anticytotoxic, genotoxic/antigenotoxic and mutagenic/antimutagenic potential of ethanolic extracts of Brazilian green propolis (EEGP) and of B. dracunculifolia (EEBD), by means of the Allium cepa test system. The effects were evaluated by assessing the chromosomal aberrations (CA) and micronuclei (MN) frequencies on meristematic and F1 generation cells from onion roots. Chemical analyses performed with the extracts showed differences in flavonoid quality and quantity. No genotoxic or mutagenic potential was detected, and both extracts were capable of inhibiting cellular damage caused by methyl methanesulfonate (MMS) treatment, reducing the frequencies of CA and MN. By these data, we can infer that, independent of their flavonoid content, the extracts presented a protective effect in A. cepa cells against the clastogenicity of MMS.

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Antimutagenicity Screening of Extracts from Medicinal and Edible Plants against N-Methyl-N-nitrosourea by the Ames Assay

Cited by 4 publications

References 68 publications

Safety studies of LAURESH<sup>®</sup> a standardized <i>Laurus nobilis</i> leaf extract

Safety studies of LAURESH<sup>®</sup> a standardized <i>Laurus nobilis</i> leaf extract

Isolation and characterization of antimutagenic components of Glycyrrhiza aspera against N-methyl-N-nitrosourea

Antigenotoxicity and antimutagenicity of ethanolic extracts of Brazilian green propolis and its main botanical source determined by the Allium cepa test system

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