Antimutagenicity profiles for some model compounds

Waters, Michael D.; Brady, Ann L.; Stack, H.Frank; Brockman, Herman E.

doi:10.1016/0165-1110(90)90039-e

Cited by 261 publications

(129 citation statements)

References 96 publications

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“…(5,(12)(13)(14)(15)(16)(17)(18)(19) The findings of this study demonstrated that A.a. presents characteristics similar to previously reported results on studies of tucumã, even in its various derivative forms in which it was examined. (7,8,19,20) Substances, such as carotenoids and polyphenols found in the oil of tucumã, were previously evaluated by de Rosso and Mercadante, (5) and Gonçalves et al, (8) who associated these compounds with the elimination of free radicals.…”

Section: Discussionsupporting

confidence: 50%

“…The micronucleus distribution among the cells may have value in identification of the mechanism, so, for its determination, 2,000 PCEs per animal were analyzed in the peripheral blood samples (24h and 48h), and a total of 400 erythrocytes per animal were analyzed to calculate the Nuclear Division Index (IDN) (15)(16)(17) using the formula: IDN = ___PCE___ PCE + NCE The percentage of frequency reduction of PCEMNs was calculated according to Waters et al (1990), (18) to determine the effectiveness of treatments for antigenotoxic effect, using the following formula:…”

Section: Analysis Of the Glass Slidesmentioning

confidence: 99%

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Efeito da Astrocaryum aculeatum (Tucumã) na toxicidade da Doxorrubicina: modelo experimental in vivo

et al. 2017

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Objective: To obtain the oil of Astrocaryum aculeatum (A.a), and evaluate its genotoxicity/antigenotoxicity activities using the micronucleus test in peripheral blood cells. Methods: The oil of Astrocaryum aculeatum was obtained by hydraulic pressing. The animals used were healthy Swiss male mice, at 6-7 weeks of age; there were six per group. The genotoxic and antigenotoxic activity of concentrations were 500, 1,000 and 2,000 mg/kg per 0.5 mL (oral), followed or not followed by intraperitoneal injection of doxorubicin (0.3 mL-15 mg/kg by body weight), in addition to a negative group (water) and dimethyl sulfoxide (600 μL). Peripheral blood samples were collected 24h and 48h after treatment. Results: A statistically significant reduction was identified in the frequency of micronuclei in polychromatic cells ranging from 34.72% to 38.19% for 24-hour treatments, and from 63.70% to 66.12% for 48 hour. Conclusion: The fixed oil of tucumã presented antigenotoxic potential for the concentrations used in acute treatments. ResumoObjetivo: Obter o óleo do Astrocaryum aculeatum (A.a) e avaliar a genotoxidade/antigenotoxidade pelo teste do micronúcleo em células do sangue periférico. Métodos: O óleo da A.a foi obtido por prensagem hidráulica. Os animais foram camundongos Swiss, machos e saudáveis com 6-7 semanas de idade, 6 por grupo. Teste genotóxico e antigenotóxico as concentrações foram de 500, 1.000 e 2.000 mg/kg por 0,5 mL (via oral), seguidas ou não de injeção intraperitoneal de doxorrubicina (0,3mL -15 mg/kg por peso corporal), além do grupo negativo (água) e dimetilsufóxido (600 µL). As amostras de sangue periférico foram coletadas 24h e 48h após o tratamento. Resultados: Houve redução estatisticamente significativa na frequência de micronúcleos em células policromáticas que variou de 34,72% à 38,19% para os tratamentos de 24h, e de 63,70 à 66,12% para os de 48h. Conclusão: O óleo fixo do tucumã apresentou potencial antigenotóxico para as concentrações em tratamentos agudos.

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Section: Discussionsupporting

confidence: 50%

Section: Analysis Of the Glass Slidesmentioning

confidence: 99%

Efeito da Astrocaryum aculeatum (Tucumã) na toxicidade da Doxorrubicina: modelo experimental in vivo

et al. 2017

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“…The DR% by MEST of damage caused by the mutagenic and/or genotoxic agent was performed as suggested by Manoharan and Banerjee (1985) and Waters et al (1990) …”

Section: Calculating the Dr%mentioning

confidence: 99%

Antigenotoxic and antimutagenic effects of Schinus terebinthifolius Raddi in Allium cepa and Swiss mice: A comparative study

Fedel-Miyasato¹,

Formagio²,

Auharek³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. It is estimated that 60% of anticancer drugs are derived directly or indirectly from medicinal plants. Schinus terebinthifolius Raddi (Anacardiaceae) is traditionally used in Brazilian medicine to treat inflammation, ulcers, and tumors. Because of the need to identify new antimutagenic agents and to determine their mechanism of action, this study evaluated the chemopreventive activity of the methanolic extract from leaves of S. terebinthifolius (MEST) in Allium cepa cells and in Swiss mice analyzing different protocols of MEST in association with DNA-damaging agents. The antigenotoxic and antimutagenic aspects in peripheral blood were evaluated using the comet and micronucleus assays, respectively. The percentage of damage reduction was used to compare the A. cepa and mice results. Our results showed for the first time that MEST can act as a chemopreventive compound that promotes cellular genome integrity by desmutagenic and bioantimutagenic activities in vegetal and animal models. This finding may therefore have therapeutic applications that can indirectly correlate to the prevention and/or treatment of the degenerative diseases such as cancer.

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“…The percent reduction in the number of cells treated with extracts that showed antimutagenic effect was calculated according to Manoharan and Banerjee (1985) and Waters et al (1990) …”

Section: Micronucleus Testmentioning

confidence: 99%

Genotoxic and antigenotoxic effects of organic extracts of mushroom Agaricus blazei Murrill on V79 cells

et al. 2005

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Agaricus blazei Murrill, popularly known as the sun mushroom, is a native mushroom in SP, Brazil, that has been widely used in the treatment of cancer and many other pathologies in different parts of the world. A water-soluble protein-polysaccharide complex (1 ® 6)b-D-glucan has been isolated from its fruiting body that showed immune-modulation activity. From organic extracts, linoleic acid has been isolated and determined to be the main substance with antimutagenic activity. Using both the micronucleus (MN) and comet (single cell microgel electrophoresis) assays, this study determined the genotoxic and antigenotoxic potential of A. blazei (AB) obtained from commercial sources or the following strains: a) strains AB 97/29 (young and sporulated phases); b) a mixture taken from AB 96/07, AB 96/09 and AB 97/11 strains; and c) commercial mushrooms from Londrina, PR and Piedade, SP, designated as AB PR and AB SP, respectively. The extracts from these mushrooms were isolated in chloroform:methanol (3:1) and used in vitro at three different concentrations. V79 cells (Chinese hamster lung cells) were exposed to the extracts under pre-, simultaneous and post-treatment conditions, combined with methyl methanesulfonate (MMS). Under the circumstances of this study, these organic extracts did not show any genotoxic or mutagenic effects, but did protect cells against the induction of micronuclei by MMS.

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Antimutagenicity profiles for some model compounds

Cited by 261 publications

References 96 publications

Efeito da Astrocaryum aculeatum (Tucumã) na toxicidade da Doxorrubicina: modelo experimental in vivo

Efeito da Astrocaryum aculeatum (Tucumã) na toxicidade da Doxorrubicina: modelo experimental in vivo

Antigenotoxic and antimutagenic effects of Schinus terebinthifolius Raddi in Allium cepa and Swiss mice: A comparative study

Genotoxic and antigenotoxic effects of organic extracts of mushroom Agaricus blazei Murrill on V79 cells

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