Antigenotoxic and antimutagenic effects of Schinus terebinthifolius Raddi in Allium cepa and Swiss mice: A comparative study

Abstract

ABSTRACT. It is estimated that 60% of anticancer drugs are derived directly or indirectly from medicinal plants. Schinus terebinthifolius Raddi (Anacardiaceae) is traditionally used in Brazilian medicine to treat inflammation, ulcers, and tumors. Because of the need to identify new antimutagenic agents and to determine their mechanism of action, this study evaluated the chemopreventive activity of the methanolic extract from leaves of S. terebinthifolius (MEST) in Allium cepa cells and in Swiss mice analyzing different protocols of MEST in association with DNA-damaging agents. The antigenotoxic and antimutagenic aspects in peripheral blood were evaluated using the comet and micronucleus assays, respectively. The percentage of damage reduction was used to compare the A. cepa and mice results. Our results showed for the first time that MEST can act as a chemopreventive compound that promotes cellular genome integrity by desmutagenic and bioantimutagenic activities in vegetal and animal models. This finding may therefore have therapeutic applications that can indirectly correlate to the prevention and/or treatment of the degenerative diseases such as cancer.

Moquiniastrum polymorphum subsp floccosum extract: screening for mutagenic and antimutagenic activity

“…Plants are well-known as biological models for screening mutagens (Leme and Marin-Morales, 2009), and data obtained from plant systems exhibit 80% agreement with data produced by mammalian model systems (Rank and Nielsen, 1997;Fedel-Miyasato et al, 2014a). An A. cepa test enables different types of analysis, such as cell cycle dysregulation, chromosomal aberrations, nuclear changes, and micronuclei formation.…”

“…The main protocols in studies of antimutagenic modes of action are pre-treatment and simultaneous treatment, which indicate both desmutagenesis and bioantimutagenesis activity; simultaneous treatment with preincubation, which indicates desmutagenesis activity; and post-treatment, which indicates bioantimutagenesis activity (Oliveira et al, 2006(Oliveira et al, , 2007. These protocols are frequently used in pre-clinical experiments (Fedel-Miyasato et al, 2014a,b;Cantero et al, 2015;Navarro et al, 2015;Oliveira et al, 2015;Magosso et al, 2016;Martello et al, 2016;Rocha et al, 2016;Schneider et al, 2016), and have been further adapted for the A. cepa assay (Fedel-Miyasato et al, 2014a;Felicidade et al, 2014a,b;Mauro et al, 2014;Nantes et al, 2014).…”

“…After treatment, the roots were collected and fixed in Carnoy's solution for at least 6 h. They were then subjected to acid hydrolysis (1 N) and Schiff-stained for 2 h in the dark. Meristem regions were sectioned, and a 2% acetic carmine drop was added to the sections in order to prepare smear slides (Fedel-Miyasato et al, 2014a;. A total of 15,000 cells/treatment (1000 cells/slide, three replicates) were analyzed under a light microscope at 400X magnification.…”

Evaluation of the antimutagenic activity and mode of action of carrageenan fiber in cultured meristematic cells of Allium cepa

“…This study aimed to evaluate the antimutagenic activity and mode of action of carrageenan fiber in cultured meristematic cells of Allium cepa, which is currently considered an important method for screening natural antimutagenic compounds (Fedel-Miyasato et al, 2014;Mauro et al, 2014).…”

Acrocomia aculeata prevents toxicogenetic damage caused by the antitumor agent cyclophosphamide

“…1.2603.0056.002-1; Batch 063020, Brazil), an indirect alkylating agent, was used as a positive control to induce DNA damage. Cyclophosphamide was prepared in normal saline (0.9% NaCl), pH 7.4, and applied in a single dose of 100 mg per kg body weight (bw) via intraperitoneal (ip) injection (Fedel-Miyasato et al, 2014a).…”