Antimicrorobial evaluation of sodium hypochlorite and ozonated water on E. faecalis biofilm

Abstract

This study aim to evaluate antimicrobial efficacy of sodium hypochlorite (NaOCl) and ozonated water against Enterococci faecalis biofilm. The bacterial biofilm was exposed to 2.62%, 1.31% NaOCl and 0.1 ppm ozonated water over a range of time periods. The presence of viable cells was determined by enumeration of colony forming units (CFU). All experiments were repeated four times (n=4). The effectiveness of the agents was compared using nonparametric Kruskal-Wallis test. The result revealed that 2.62% of NaOCl can completely kill E. faecalis biofilm in 15 minutes whereas 1.31 % NaOCl required a longer time to produce such effect. 0.1 ppm ozonated, however, did not exhibit any antimicrobial effect within the period of time tested. From this study, it can be concluded that 0.1 ppm ozonated water was not comparable with 2.62% and 1.31 % NaOCl in antimicrobial efficacy against E.faecalis biofilm.

Root Canal Irrigants in Primary Teeth

“…3 Although it is a powerful antimicrobial agent, less attention has been paid to its antibacterial activity on bacterial biofilm and hence in root canal infection. 34,35 Studies have found that when the specimen was irrigated with sonication killing ability of ozonated water and 2.5% sodium hypochlorite was almost comparable, However, it was found that effect on E. faecalis was more with NaOCl than ozonated water in broth culture and in biofilm. Hence concluded that there is need for further studies and modifications in ozonated water before it could be used as a root canal irrigant.…”

Newer Root Canal Irrigants in Horizon: A Review

“…Ibrahim and Abdullah studied that 1.31% NaOCI might allow passage of oxidation of ozonated water, thus increasing their antibacterial effect compared to 1.31% NaOCI or ozonated water alone [67]. …”

“…The concentration of ozone in ozonated water can be measured using a dissolved ozone meter. Although ozonated water is a powerful antimicrobial agent against bacteria, fungi, protozoa, and viruses, less attention has been paid to the antibacterial activity of ozonated water in bacterial biofilm and hence in root canal infection [ 66 , 67 ].…”

“…Study by Hems et al however found that NaOCI was superior to ozonated water in killing E. faecalis in broth culture and in biofilm [ 73 ]. Ibrahim and Abdullah studied that 1.31% NaOCI might allow passage of oxidation of ozonated water, thus increasing their antibacterial effect compared to 1.31% NaOCI or ozonated water alone [ 67 ].…”

Ozonated water antimicrobial efficacy in comparison to antimicrobial activity of different irrigant solutions against E. faecalis and E. coli

“…It is this particular reactivity that provides the starting point for ozone's therapeutic effects on the body [17] . Although ozonated water is a powerful antimicrobial agent against bacteria, fungi, protozoa and viruses, less attention has been paid to the antibacterial activity of ozonated water in bacterial biofilm and hence in root canal infection [18] . Therefore the aim of this study was to determine the antimicrobial efficacy of ozonated water and compare the antimicrobial activity of different irrigant solutions against E. faecalis and E. coli.…”