Ozonated water antimicrobial efficacy in comparison to antimicrobial activity of different irrigant solutions against E. faecalis and E. coli

Abstract

to test and compare the efficacy of Ozonated Water, 3% Sodium Hypochlorite and Normal Saline in relation to Enterococcus faecalis (MTCC 439) and Escherichia coli (ATCC 25922) micro-organisms. After incubation, 21 contaminated roots were divided into 3 groups according to the irrigation regimen used i.e. Group A: Ozonated Water (7 teeth), Group B: 3% NaOCl (7 teeth) and Group C: Saline (7 teeth). All the teeth were handled with sterile gloves and sterile tweezers to prevent contamination. A sterile 5 mL syringe with 26 gauge needle was used to deliver irrigant into the canal. All experimental teeth were then flushed with distilled water to prevent potential carry-over of irrigants. Results: Results indicated that Group C showed highly significant difference from group A and Group B indicating that Group A and Group B were effective against both E. faecalis and E. coli. Results also show that there was no significant difference in efficacy of Group A and Group B. Conclusion:The results found that ozonated water has proved to be an alternative antimicrobial agent.