Antimicrobial Susceptibility of Autochthonous Aquatic Vibrio cholerae in Haiti

Baron, Sandrine; Lesné, Jean; Jouy, Éric; Larvor, Emeline; Kempf, Isabelle; Boncy, Jacques; Rebaudet, Stanilas; Piarroux, Renaud

doi:10.3389/fmicb.2016.01671

Cited by 30 publications

(19 citation statements)

References 39 publications

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“…The observation of resistance amongst the V. cholerae to these antibiotic group is a potential determinant of epidemiological relevance especially when considering future strategies for management of cholera cases. Similar to the above observation is the reports from other clinical/environmental studies in the Asian continent (China, Mumbai, North India) (outside Africa) and other regions (Haiti) which have also reported similar occurrence of resistance to the cephalosporin group of antibiotics [ 59 , 76 , 77 , 78 ]. Their studies accessed water and stool samples and observed both O1/O139 and non-O1/nonO139 V. cholerae strains of diverse virulent and multiple antibiotic resistant determinants.…”

Section: Discussionsupporting

confidence: 90%

Antibiotic Susceptibility Testing (AST) Reports: A Basis for Environmental/Epidemiological Surveillance and Infection Control Amongst Environmental Vibrio cholerae

Igere

Okoh

Nwodo

2020

IJERPH

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Distribution, investigation, surveillance and control (DISC) of cholera outbreaks in endemic/non-endemic regions has been a concerted approach towards the management of the causal pathogen. Relevant organization, government, health systems and the public have implemented several steps towards controlling the menace, yet pathogen continues to occur with diverse phenotypes/genotypes of high clinical and epidemiological relevance. The study determines antibiotic susceptibility/resistance pattern of Vibrio cholerae isolates retrieved from six domestic water sources between March and August 2018. Serological and molecular typing methods (polymerase chain reaction or PCR) were used to confirm the isolates identity. Antibiotic susceptibility testing was conducted using six commonly employed antibiotics of V. cholerae according to the recommendation of Clinical Laboratory Standard and European Committee for Antimicrobial Susceptibility Testing with other relevant antibiotics of investigative epidemiology and infection control, employing both disc diffusion test and PCR gene detection. Samples presumptive counts ranged between 1.10 to 7.91 log10 CFU/mL. Amongst the 759 presumptive isolates retrieved, sixty-one were confirmed as V. cholerae which were further serogrouped as Non-O1/Non-O139 V. cholerae. Various V. cholerae resistant phenotypes/genoytypes were detected vis: carbapenemase (CR-Vc; 31.1%/5.3%). New Delhi Metallobetalactamase (NDM-1-Vc; 23.0%/42.5%), extended spectrum betalactamase (ESBL-Vc; 42.6%/blaTEM:86,7%), chloramphenicol resistance (62.3%/Flor: 46.2%}, tetracycline resistance (70.5%/46.7%), AmpC resistance (21.0 (34.4%/56.7%)) and various other resistant genotypes/phenotypes. It was observed that more than 50% of the confirmed V. cholerae isolates possess resistance to two or more antibiotic classes/groups with multiple antibiotic resistance index (MARI) ranging from 0.031 to 0.5. This observation provides necessary information and updates for surveillance, planning and implementation of control strategies for cholera. It would also encourage decision making, formulation of policy by the government and cholera control authorities.

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Section: Discussionsupporting

confidence: 90%

Antibiotic Susceptibility Testing (AST) Reports: A Basis for Environmental/Epidemiological Surveillance and Infection Control Amongst Environmental Vibrio cholerae

Igere

Okoh

Nwodo

2020

IJERPH

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“…A recent study found no ermA gene and 90% of V. cholerae isolates harboured the ermB gene. 57 The present study found that isolates showed no amplification for the ermA and ermB genes. So, erythromycin is a good choice for treating cholera infections.…”

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confidence: 41%

In silico Assessment of the Genotypic Distribution of Virulence and Antibiotic Resistance Genes in Vibrio cholerae

Nahar

Rashid

2017

Dhaka Univ. J. Pharm. Sci

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Vibrio cholerae has long been reported as an important cause of death in developing countries. The study detected the virulence and antibiotic resistance gene of eight V. cholerae isolates through in silico tools. Cholera toxins, ctxA and ctxB were found in six isolates (75%). Seventy-five percent isolates were also found to be positive for zonula occludens toxin, zot which is known to increase the permeability by altering the tight junction of the small intestine. Accessory cholera enterotoxin ace, responsible for fluid accumulation, was detected in four V. cholerae strains. Seven isolates (87.5%) were positive for toxin-coregulated pilus, tcp which helps the bacteria to adhere to gut mucosa. Both ompW and toxR genes were found in 87.5% of the isolates. Twenty-five percent isolates harboured strA, strB, sulII, dfrA1, floR genes and SXT element demonstrating that they were multidrug-resistant (MDG) bacterium. One isolate was found to be positive for tetA gene while no erythromycin resistance gene, ermA and ermB was found. Virulence genes were found in all genotypes indicating that their distribution was not genotypeoriented while genotype 2 harboured no antibiotic resistance genes. This data helps to predict virulence genes associated with cholera and also demonstrates the presence of antibiotic resistance genes. Bacteria acquired the antibiotic resistance gene through natural process which cannot be stopped. So by analyzing the resistance pattern we can choose appropriate antibiotics. In silico study helps us to predict the antibiotic resistant genotypes and can easily identify antibiotic resistant strains which help us to treat cholera infections and reduce the morbidity and mortality rate of the infected individuals.

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“…For doxycycline and erythromycin, no breakpoint were available, based on the distribution of the diameter of inhibition (Figures 4A,B ), only one population could be observed. All the strains were considered as susceptible (Baron et al, 2016 ).…”

Section: Resultsmentioning

confidence: 99%

Antimicrobial Susceptibility among Urban Wastewater and Wild Shellfish Isolates of Non-O1/Non-O139 Vibrio cholerae from La Rance Estuary (Brittany, France)

et al. 2017

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The early 2000s marked the end of the Golden age of the antibiotics and the beginning of the awareness on the potential threat to human health due to the dissemination of antimicrobial resistance. As a base-line study, we investigated the antimicrobial susceptibility of 99 strains of non-O1/non-O139 Vibrio cholerae isolated from wastewater and shellfish in 2000/2001 within La Rance estuary (Brittany, France). All isolates were susceptible to amoxicillin-clavulanic acid, cefotaxime, imipenem, chloramphenicol, nalidixic acid, ciprofloxacin, norfloxacin, amikacin, gentamicin, tetracycline, doxycycline, trimethoprim-sulfamethoxazole, and erythromycin. The only resistances were to streptomycin, sulfonamides and ampicillin: 54.6% of the isolates had acquired resistance to at least one antimicrobial agent among them and only six isolates from cockles were multidrug resistant. On the basis of the distribution of a limited selection of resistance associated genes, our study shows that V. cholerae can constitute an environmental reservoir for these genes. However, none of our isolates harbored integron. This result casts doubt on the capacity of non-O1/non-O139 V. cholerae to acquire resistance-associated genes in such context, and on its potential role of indicator of the dissemination of antimicrobial resistance in the aquatic environment.

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Antimicrobial Susceptibility of Autochthonous Aquatic Vibrio cholerae in Haiti

Cited by 30 publications

References 39 publications

Antibiotic Susceptibility Testing (AST) Reports: A Basis for Environmental/Epidemiological Surveillance and Infection Control Amongst Environmental Vibrio cholerae

Antibiotic Susceptibility Testing (AST) Reports: A Basis for Environmental/Epidemiological Surveillance and Infection Control Amongst Environmental Vibrio cholerae

In silico Assessment of the Genotypic Distribution of Virulence and Antibiotic Resistance Genes in Vibrio cholerae

Antimicrobial Susceptibility among Urban Wastewater and Wild Shellfish Isolates of Non-O1/Non-O139 Vibrio cholerae from La Rance Estuary (Brittany, France)

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