Antimicrobial Susceptibility among Urban Wastewater and Wild Shellfish Isolates of Non-O1/Non-O139 Vibrio cholerae from La Rance Estuary (Brittany, France)

Abstract

The early 2000s marked the end of the Golden age of the antibiotics and the beginning of the awareness on the potential threat to human health due to the dissemination of antimicrobial resistance. As a base-line study, we investigated the antimicrobial susceptibility of 99 strains of non-O1/non-O139 Vibrio cholerae isolated from wastewater and shellfish in 2000/2001 within La Rance estuary (Brittany, France). All isolates were susceptible to amoxicillin-clavulanic acid, cefotaxime, imipenem, chloramphenicol, nalidixic acid, ciprofloxacin, norfloxacin, amikacin, gentamicin, tetracycline, doxycycline, trimethoprim-sulfamethoxazole, and erythromycin. The only resistances were to streptomycin, sulfonamides and ampicillin: 54.6% of the isolates had acquired resistance to at least one antimicrobial agent among them and only six isolates from cockles were multidrug resistant. On the basis of the distribution of a limited selection of resistance associated genes, our study shows that V. cholerae can constitute an environmental reservoir for these genes. However, none of our isolates harbored integron. This result casts doubt on the capacity of non-O1/non-O139 V. cholerae to acquire resistance-associated genes in such context, and on its potential role of indicator of the dissemination of antimicrobial resistance in the aquatic environment.

Detection of Multidrug-Resistance Gram-Negative Bacteria from Hospital Sewage in North East, Nigeria

“…In the current study, results also found isolates of significant health importance that show high degree of multidrug resistance and analysis of the MAR index of isolates showed that most of the isolates had MARI of >0.2, this serves as a pointer to high use of antibiotics in such environments. The high rates of resistance by these isolates in this study agreed with the previous studies [42,[49][50][51][52]. These isolates should raise concern as they play role in many hospital infections.…”

Isolation and antibiotic profile ofVibriospp. in final effluents of two wastewater treatment plants in the Eastern Cape of South Africa

“…This study recorded 58% susceptibility level with 29% resistance to ampicillin. This compared favorably with other studies which exhibiting resistance to ampicillin [61,80,81]. Furthermore, Igbinosa, [12] and Quilici et al, [71] reported intermediate susceptibility to ampicillin while Ismail et al, [63] and Tran et al, [67] reported high susceptibility level to ampicillin from Vibrio cholera tested in South Africa and Vietnam.…”

<p>Non-O1, Non-O139 <em>Vibrio cholerae</em> (NOVC) Bacteremia: Case Report and Literature Review, 2015–2019</p>

“…22,23 Many NOVC stains have been isolated in the coastal waters of countries such as Germany, India, South Korea, and France. [24][25][26][27] Our review found that 47.8% of patients (11/23) had a possible source of infection; 6 cases were attributed to consuming seafood and 4 to contaminated water. Two cases presented swelling or pain in the lower leg after contact with contaminated water through a wound.…”