Antimicrobial Susceptibility and Detection of Virulence-Associated Genes in Escherichia coli Strains Isolated from Commercial Broilers

Kocúreková, Tímea; Karahutová, Lívia; Bujňáková, Dobroslava

doi:10.3390/antibiotics10111303

Cited by 3 publications

(2 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2). The results agree with those of Kocúreková et al [16] in Slovakia who found Tobramycin E. coli resistance from broilers in 1.96%. The most common mechanism of acquired resistance to aminoglycosides is the modification of the chemical structure of the antibiotic by bacterial enzymes.…”

Section: Tobramycin E Coli Resistance Before Covid-19 Appearancesupporting

confidence: 92%

“…Animal products may contain antimicrobial resistant bacteria as a result of fecal contamination during slaughter [32]. Commercial broilers can be reservoirs of virulent and resistant genes as well as Escherichia coli causing extra-intestinal infections, which can be a potential threat to humans via direct contact and food [16]. During the processing of meat, the bacteria from animal origin can contaminate other food items, the processing plant, or workers.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Emergence of Tobramycin Escherichia coli resistance in poultry meat linked to biocides overuse during COVID-19

Guergueb

Alloui

2023

RC FCV-LUZ

View full text Add to dashboard Cite

The effect of excessive use of biocides during the COVID-19, on the resistance of Escherichia coli to Tobramycin in poultry, meat was examined in this observational epidemiological study (Before and after COVID–19). Tobramycin E. coli resistant strains isolated from poultry meat before COVID-19 appearance were compared with those isolated after COVID-19 emergence. Univariable analyses were performed using t-test and chi-squared test. Odds ratios and 95% confidence intervals were used for statistically significant risk factor. Multivariate analysis was done with the binary logistic regression to detect an independent predictor, and with the principal component analysis (PCA), to analyze whether the Tobramycin resistance in E. coli was linked with the COVID-19 outbreak. Statistical significance was set at P<0.05. The frequency of Tobramycin E. coli resistant isolates was more important after COVID-19 emergence (12.5%) than before COVID-19 (2.1%). Graphical representation of PCA qualitative variables shows the interfactor relationship. A significant relationship between Tobramycin E. coli resistance and COVID-19 emergence (P=0.014), and the effect of the emergence of COVID-19 on the Tobramycin E. coli resistance was OR = 6.57 (95% Confidence interval (CI) 1.61-7.94). The probability of Tobramycin E. coli resistance linked with poultry meat bought after COVID-19 was 1.88 times more than before COVID-19 emergence. Poultry meat purchased after COVID-19 found related to Tobramycin resistance in E. coli. It seems possible that the overuse of biocides during COVID-19 increased the risk of Tobramycin E. coli resistance in poultry meat.

show abstract

Section: Tobramycin E Coli Resistance Before Covid-19 Appearancesupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Emergence of Tobramycin Escherichia coli resistance in poultry meat linked to biocides overuse during COVID-19

Guergueb

Alloui

2023

RC FCV-LUZ

View full text Add to dashboard Cite

show abstract

Bacteriophages Mediating Effective Elimination of Multidrug-Resistant Avian Pathogenic Escherichia coli

Koratkar,

Bhutada,

Giram

et al. 2024

PHAGE

View full text Add to dashboard Cite

Study of Antibacterial Chemical Substances and Molecular Investigation among Sulfamethoxazole-trimethoprim (SXT)- Resistant Escherichia coli Isolates

Ali¹,

Alshara

Tuwaij

et al. 2022

rbmb.net

View full text Add to dashboard Cite

Background: Escherichia coli (E. coli) remains one of the leading agents of urinary tract infection (UTIs), it has become resistant to many drugs. Current work aimed to evaluate some chemical substances as antibacterial agents and molecular study of virulence factors associated with UTIs. Methods: This work involved 133 urine specimens obtained from females' patients suffering from UTIs, Methods of well diffusion and disk diffusion were achieved to assay the effect of some chemical substances and antibiogram profiles toward Sulfamethoxazole-trimethoprim (SXT)resistant E. coli respectively. Virulence genes were done based on the technique of Polymerase Chain Reaction (PCR). Results:The results recorded 49/133 (36.84%) E. coli among women suffering UTIs, 28/49 (57.14%) were resistant to SXT drug. imipenem, meropenem, and nitrofurantoin were recorded more effectively. Chemicals substances at the concentration 0.3 (g/ml) recorded percentages of inhibition, reaching 9.143±1.442, 15.36±0.5914, and 21.82±0.8699 for NaHCO3, Ch4c, and Viroxide Super™ respectively. PCR demonstrated that 28/28 (100%) of SXT-resistant E. coli isolates were harbored Sul-2, FeoB and PapC genes, while 14/28 (50%), 15/28 (53.57%), 19/28 (67.85%) and 26/28 (92.85%) in U250 (pet), FumC, Sul-1 and IutA genes, respectively. Sul-3 gene was not observed. Conclusions: Observed a high percentage of E. coli that were resistant to SXT drug, and having several virulence genes, poses a real threat, it requires a real pause to create substitutions to limit the spreading of this threat.

show abstract

Antimicrobial Susceptibility and Detection of Virulence-Associated Genes in Escherichia coli Strains Isolated from Commercial Broilers

Cited by 3 publications

References 59 publications

Emergence of Tobramycin Escherichia coli resistance in poultry meat linked to biocides overuse during COVID-19

Emergence of Tobramycin Escherichia coli resistance in poultry meat linked to biocides overuse during COVID-19

Bacteriophages Mediating Effective Elimination of Multidrug-Resistant Avian Pathogenic Escherichia coli

Study of Antibacterial Chemical Substances and Molecular Investigation among Sulfamethoxazole-trimethoprim (SXT)- Resistant Escherichia coli Isolates

Contact Info

Product

Resources

About