Study of Antibacterial Chemical Substances and Molecular Investigation among Sulfamethoxazole-trimethoprim (SXT)- Resistant Escherichia coli Isolates

Ali, Ruaa Ali Muhammed; Alshara, Jamal M. R.; Tuwaij, Nabil Salim Saaid; AL-Khilkhali, Huda Jameel Baker

doi:10.52547/rbmb.11.1.166

Cited by 3 publications

(3 citation statements)

References 35 publications

(23 reference statements)

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“…A study done by Khurshid et al (18) found that frequency of sul-1 and sul-2 genes among 131 isolates of SXT-resistant A. baumannii were 10.7% and 72.5% respectively, while 16% of isolates have been both sul-1 and sul-2. However, this result were closer to a local study done by Muhammed et al (25) in Al-Najaf City, they observed high spreading of sul-1 and sul-2 but sul-3 was not detected among SXT-resistant E. coli isolates.…”

Section: Discussionsupporting

confidence: 86%

Genetic Detection of Folate Pathway Inhibitors Genes among Acinetobacter spp. Isolates

Al-Ghazaly¹,

Tuwaij²

2022

The Egyptian Journal of Hospital Medicine

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Background: Exist of folate pathway inhibitor genes among Acinetobacter spp. isolates are regarded as a significant mechanism of resistance for sulfa drugs in this pathogen. Objective: This study aimed to investigate genes associated with sulfa drugs resistance among Acinetobacter spp. using Polymerase Chain Reaction (PCR). Patients and Methods: This study included 928 specimens from patients who visited the main hospitals and private clinic laboratories of Al-Najaf City-Iraq. All specimens were cultivated and bacterial diagnosis was done according to standard methods. Antibiotics susceptibility and molecular investigation for sul-1, sul-2, sul-3, dfr-A, dfr-B, dfr-G and dfr-K for all Acinetobacter spp. isolates were done. Results: The rate of Acinetobacter spp. isolates were 28 (5.4%). Results showed a high resistance towards antibiotics classes, 28 (100%) of isolates were resistance to piperacillin, cefotaxime, ceftriaxone, ceftazidime and cefepime, while the lowest resistance rate was against minocycline reached 42.85. PCR showed 28 (100%) of Acinetobacter spp isolates were harbored sul-1 and drf-G genes. 25 (89.29%) and 21 (75%) of isolates were positive for sul-2 and drf-A genes respectively, while sul-3, drf-B and drf-K genes were not detected. Conclusion: There is a great deal of concern about antimicrobial agent resistance and also about the number of drugassociated resistance genes contained in Acinetobacter spp. isolates especially sul-1, sul-2, dfr-A, and dfr-G, which have a significant role in sulfa drug resistance.

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Section: Discussionsupporting

confidence: 86%

Genetic Detection of Folate Pathway Inhibitors Genes among Acinetobacter spp. Isolates

Al-Ghazaly¹,

Tuwaij²

2022

The Egyptian Journal of Hospital Medicine

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“…Also, a previous report indicated that E. coli isolates demonstrated notable resistance to LEV and DO, with resistance rates of 18% and 37% respectively (Aly, Essam & Amin 2012). Moreover, in previous study, the E. coli isolates showed 85.7% resistance against PRL and 100% resistance against SXT (Ali et al 2022). Based on the current findings, it is possible to attribute the high susceptibility rates of AK and MRP to the accurate selection of these antibiotics for therapeutic purposes, as this approach ensures the preservation of the antibiotics' efficacy.…”

Section: Discussionsupporting

confidence: 58%

Mitigation of Virulence in Resistant Escherichia Coli by Diclofenac Sodium, Phenotypic and Genotypic Study.

M. Eltabey,

H. Ibrahim,

M. Zaky

et al. 2024

Delta University Scientific Journal

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Escherichia coli is one of the most ubiquitous pathogens causing several life-threatening diseases. Currently, antibiotics are failing fast while rates of drug-resistant bacteria are increasing worldwide with almost no new drugs coming to market and even last-resort antibiotics are becoming ineffective. Anti-virulence is a new approach targeting bacterial virulence and unlike classic antibiotics is aimed at disarming pathogens rather than killing them or inhibiting their growth. Inactivation of the bacterial arsenal of virulence factors will attenuate pathogens and render them susceptible to natural host defenses. The effect of the FDA-approved drug diclofenac sodium at a sub-inhibitory concentration (¼ MIC) on some Escherichia coli virulence factors was evaluated phenotypically and genotypically. Phenotypically, biofilm formation ability, proteases production and motility behavior were found to be significantly decreased under the effect of diclofenac sodium. Genotypically, qRT-PCR was used to evaluate the relative expression levels of papC, fimH, ompT_m, stcE and flic genes regulating virulence factors production. Importantly, the relative expression levels of all the tested genes showed significant downregulation under the effect of diclofenac sodium. The current research revealed that diclofenac sodium has a promising anti-virulence effect against resistant Escherichia coli. As a result, it is suggested that, in addition to or as an adjuvant to traditional antimicrobials, diclofenac sodium could be a possible solution to treat infections caused by resistant Escherichia coli.

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“…Furthermore, the diameter of its inhibition zone was greater for S. aureus than for E. coli. Genes of E. coli were associated with main virulence factors such as adhesins, toxins with increased pathogenicity and caused human infection [14]. The resistance level of gram-negative bacteria was higher than its grampositive counterpart [15].…”

Section: Discussionmentioning

confidence: 99%

The Activity of α-glucosidase Inhibition of Pediococcus Acidilactici BAMA 4 Isolated from “Naniura” Traditional Foods from North Sumatera, Indonesia

Riastawaty,

Girsang,

Fachrial

et al. 2023

TOBIOCJ

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Background: Type 2 diabetes is caused by unhealthy lifestyles, such as consuming foods rich in simple sugars and lack of exercise. One of the treatment therapies for this disease is α-glucosidase inhibitors. Some strains of the lactic acid bacteria (LAB) group typically exhibit α-glucosidase inhibitory activity, antimicrobial activity, acids and bile salt tolerance, and probiotic status. Objective: This study aims to isolate LAB from naniura, characterize and test its activity as an α-glucosidase inhibitor, as well as identify those with the highest activity. Methods: The method used to molecularly identify potentially good LAB was through the amplification of the 16S rRNA gene. Results: This study obtained a total of 9 strains using BAMA codes 1, 2, 3, 4, 5, 6, 7, 8, and 9. The cocci cell activity, gram-positive, and antibacterial activity of BAMA 4 against E.coli was 7.54 mm, while against S. aureus was 8.05 mm. The percentage of viability in acid and bile salt is 28.7% and 68.6%, respectively. Additionally, the proportion of α-glucosidase inhibition is 65%. Discussion: The BAMA 4 strain is a species of Pediococcus acidilactici. Pediococcus is a genus of gram-positive lactic acid bacteria in the Lactobacillacea family. Conclusion: BAMA 4 strain produced antibacterial optimally and its cells survived the growth in acid and bile conditions. The percentage of probiotics was relevantly high in this activity. This study will be helpful for other in-vivo research.

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Study of Antibacterial Chemical Substances and Molecular Investigation among Sulfamethoxazole-trimethoprim (SXT)- Resistant Escherichia coli Isolates

Cited by 3 publications

References 35 publications

Genetic Detection of Folate Pathway Inhibitors Genes among Acinetobacter spp. Isolates

Genetic Detection of Folate Pathway Inhibitors Genes among Acinetobacter spp. Isolates

Mitigation of Virulence in Resistant Escherichia Coli by Diclofenac Sodium, Phenotypic and Genotypic Study.

The Activity of α-glucosidase Inhibition of Pediococcus Acidilactici BAMA 4 Isolated from “Naniura” Traditional Foods from North Sumatera, Indonesia

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