ANTIMICROBIAL STUDY AND SYNTHESIS OF MIXED LIGAND COMPLEX OF Ni(II) ION DERIVED FROM ISATIN SCHIFF BASE AND HISTIDINE

Sharma, Shobhana; Yadav, Poonam; Seema, None; Kumari, Suman; Ranka, Mamta

doi:10.31788/rjc.2022.1526584

Cited by 4 publications

(4 citation statements)

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“…Then incubated for 30 minutes, analyzed with a microplate reader and the data is ready to be processed. The value of % inhibition [6], [7] is calculated by the following formula: % Inhibition = ((A_control-A_sample))/A_control x 100)…”

Section: Antioxidant Testmentioning

confidence: 99%

“…After a 24-hour incubation period, the diameter of the zone that inhibited bacterial growth was assessed. Every treatment were carried out aseptically and repeated twice [6]- [10].…”

Section: Antibacterial Testmentioning

confidence: 99%

“…People frequently use Solanum ferox to cure a variety of illnesses, including syphilis, bodily discomfort, loss of appetite, fever, itching, wounds, bruising, and antipyretics. According to reports, Solanum ferox has pharmacological properties like antioxidant and antibacterial [6]. Streptococcus pyogenes (S.pyogenes) and Staphylococcus aureus (S. aureus).…”

Section: Introductionmentioning

confidence: 99%

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Isolation of Antioxidant and Antibacterial Compound from Solanum ferox L Leaves

Hazimah,

Haiyul,

Dina

et al. 2023

BIO Web Conf.

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The sour eggplant plant (Solanum ferox L), a vegetable that is frequently used in cooking and is a member of the Solanaceae family, is also used as an antipyretic and to treat syphilis, body aches, lack of appetite, fever, itching, cuts, and bruising. This is due to the presence of several bioactive chemicals that have toxic, rheumatoid, antiviral, anticancer, antibacterial, and anti-asthma properties. The objective of this work was to separate antioxidant and antibacterial substances from Solanum ferox L. leaves. N-hexane and methanol were used to extract the leaves of Solanum ferox. The characterization of chemicals in the n-hexane extract was done while the antioxidant and antibacterial properties of both extracts were examined. UV-Vis spectra, FTIR, proton and carbon nuclear magnetic resonance (1H-NMR and 13C-NMR), and FTIR were used to characterize the compound). Using vitamin C as a reference and the DPPH (1,1-diphenyl-2-picrylhydrazyl) technique to measure free radicals, the antioxidant activity of the Solanum ferox plant extract was assessed. E. coli, S. aureus, and S. subtilis bacteria are used in an agar diffusion method test for antimicrobial activity. The solvent used to dissolve the sample served as the negative control while amoxsan served as the positive standard. One pure substance that has been isolated with success is stigmasterol, with the chemical formula C29H48O. The IC50 of Solanum ferox leaf n-hexane extract at 1218.5 ppm, which included not active, indicated that the IC50 of Solanum ferox leaf methanol extract at 268.5 ppm was ineffective. E. coli is inhibited by n-hexane extract in antibacterial tests at an inhibition value of 9.27 mm. Methanol extracts had an inhibitory potency of 7.82 mm against E. coli and 11.29 mm against S. aureus.

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Section: Antioxidant Testmentioning

confidence: 99%

“…After a 24-hour incubation period, the diameter of the zone that inhibited bacterial growth was assessed. Every treatment were carried out aseptically and repeated twice [6]- [10].…”

Section: Antibacterial Testmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Isolation of Antioxidant and Antibacterial Compound from Solanum ferox L Leaves

Hazimah,

Haiyul,

Dina

et al. 2023

BIO Web Conf.

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“…Modified methods [16] were used for the test of antibacterial activity. Testing the antibacterial activity of the extract and fraction of tamoenju leaves against S. aureus and S. typhi was carried out using the agar diffusion method.…”

Section: Antibacterial Activity Testmentioning

confidence: 99%

Antibacterial Activity and Bioautographic Evaluation of Extract and Fraction From Tamoenju (Hibiscus Surattensis L.) Leaves

YULIET

KHUMAIDI²,

HIKMA³

et al. 2022

Int J App Pharm

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Objective: The tamoenju (Hibiscus surattensis L.) is one of the plants as traditional medicines to treat infections. Tamoenju leaves contain alkaloids, flavonoids, saponins, tannins, and steroids, a potential antibacterial agent. This study aimed to determine the antibacterial activity of tamoenju leaves extract and fraction against Staphylococcus aureus (ATCC 25923) and Salmonella typhi (ATCC 14028), and detect the active compounds using Thin Layer Chromatography (TLC) Bioautography techniques. Methods: The sample was extracted using maceration method with 96% ethanol as solvent. Fractionation of ethanol extract using the liquid-liquid extraction method using n-hexane and ethyl acetate. The agar well diffusion method was used to evaluate the antibacterial activity with various concentrations of 2.5%, 5%, 10%, and 20%, followed by TLC bioautography using n-butanol: acetic acid: aquadest (4:1:1) as the mobile phase and silica gel GF 254 as the stationary phase on the most active fraction. Zones of inhibition showed the sensitivity of the tested microorganisms. Results: The results showed the extract, n-hexane, and water fractions were more sensitive to S. typhi, while the ethyl acetate fraction was more sensitive to both bacteria. The zone of inhibition increased with the increasing extract and fractions concentration. The bioautography TLC showed that the compounds that had the potential as antibacterial in the most active fraction (ethyl acetate fraction) were flavonoids. Conclusion: The extract and fraction of tamoenju leaves have antibacterial activity. Ethyl acetate fraction had the highest antibacterial activity. The compounds predicted to have antibacterial activity against the two tested bacteria were flavonoids.

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