Antimicrobial resistance, virulence profiles, and phylogenetic groups of fecal Escherichia coli isolates: A comparative analysis between dogs and their owners in Japan

Harada, Kazuki; Okada, Erika; Shimizu, Takefumi; Kataoka, Yuki; Sawada, Takuo; Takahashi, Toshio

doi:10.1016/j.cimid.2011.12.005

Cited by 38 publications

(41 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…in dogs and cats [36,37,38,39,40,41,42], reliable quantitative data are needed to provide a better understanding of the dynamics of antimicrobial resistance transmission between household animals and humans. The assessment of risk factors that contribute to the dissemination of drug-resistant bacteria, or corresponding genetic elements, between pets and their owners is essential for the implementation of safe handling procedures of companion animals and prudent use of antimicrobial substances in veterinary medicine.…”

Section: Antimicrobial Use In Animalsmentioning

confidence: 99%

Transfer of Multidrug-Resistant Bacteria Between Intermingled Ecological Niches: The Interface Between Humans, Animals and the Environment

Costa

Loureiro

Matos

2013

IJERPH

267

189

View full text Add to dashboard Cite

The use of antimicrobial agents has been claimed to be the driving force for the emergence and spread of microbial resistance. However, several studies have reported the presence of multidrug-resistant bacteria in populations exposed to low levels of antimicrobial drugs or even never exposed. For many pathogens, especially those organisms for which asymptomatic colonization typically precedes infection (e.g., Enterococcus spp. and Escherichia coli), the selective effects of antimicrobial use can only be understood if we considerer all biological and environmental pathways which enable these bacteria, and the genes they carry, to spread between different biomes. This ecological framework provides an essential perspective for formulating antimicrobial use policies, precisely because it encompasses the root causes of these problems rather than merely their consequences.

show abstract

Section: Antimicrobial Use In Animalsmentioning

confidence: 99%

Transfer of Multidrug-Resistant Bacteria Between Intermingled Ecological Niches: The Interface Between Humans, Animals and the Environment

Costa

Loureiro

Matos

2013

IJERPH

267

189

View full text Add to dashboard Cite

show abstract

“…Although phylogenetic characterization is an important tool to improve the understanding of E. coli populations and the relationship between strains and disease (CLERMONT et al, 2011;COURA et al, 2015), few studies have determined phylogenetic groups of E. coli isolated from dogs worldwide, and of those that did, the most frequently used method was a triplex PCR method developed by CLERMONT in 2000. This method is only capable of determining phylogroups A, B1, B2, and D (HARADA et al, 2012;SALVARANI et al, 2012;SCHMIDT et al, 2015) instead of the seven phylogroups that were detected in this study. In Brazil, only two studies identified E. coli pathovars associated with diarrhea in dogs (ALMEIDA et al, 2012;PUÑO-SARMIENTO et al, 2013), but none determined the phylogenetic groups of E. coli.…”

Section: Introductionmentioning

confidence: 99%

Detection of virulence genes and the phylogenetic groups of Escherichia coli isolated from dogs in Brazil

et al. 2018

View full text Add to dashboard Cite

Detecção de genes de virulência e grupos filogenéticos de amostrasEscherichia coli isoladas de cães no Brasil INTRODUCTIONEscherichia coli are a component of normal intestinal microbiota in humans and other animals. Phenotypic and genotypic characteristics allow the identification of E. coli pathogenic strains or pathovars (COURA et al., 2014). Dogs are colonized by E. coli during the first days of their life and some strains can cause enteric or extra-intestinal infections (BEUTIN, 1999 (BEUTIN, 1999). Other pathovars are associated with diarrhea in other animals, namely enteroaggregative E. coli (EAEC) and enteroinvasive E. coli (EIEC) (MAINIL, 2013). Because of the close contact with humans, dogs could increase the risk of transmitting potentially zoonotic microorganisms, such as EPEC and STEC (BEUTIN, 1999).In addition to the pathotype and pathovar classification, E. coli strains can be assigned to one of the seven phylogenetic groups, including A, B1, B2, C, D, E, and F (CLERMONT et al., 2013). E. coli genomic structure has shown that the strains belonging to different phylogroups are associated with the disease state and source of isolation (CLERMONT et al., 2013). The extraintestinal pathogenic E. coli (ExPEC) strains are clustered mostly in groups B2 and D and intestinal pathogenic E. coli are mostly in phylogroups A, B1, and E (ESCOBAR- PÁRAMO et al., 2004; CLERMONT et al., 2011). Although phylogenetic characterization is an important tool to improve the understanding of E. coli populations and the relationship between strains and disease (CLERMONT et al., 2011;COURA et al., 2015), few studies have determined phylogenetic groups of E. coli isolated from dogs worldwide, and of those that did, the most frequently used method was a triplex PCR method developed by CLERMONT in 2000. This method is only capable of determining phylogroups A, B1, B2, and D (HARADA et al., 2012;SALVARANI et al., 2012;SCHMIDT et al., 2015) instead of the seven phylogroups that were detected in this study. In Brazil, only two studies identified E. coli pathovars associated with diarrhea in dogs (ALMEIDA et al., 2012;PUÑO-SARMIENTO et al., 2013), but none determined the phylogenetic groups of E. coli. Therefore, this study aimed to detect the virulence genes, pathovars, and phylogenetic groups of E. coli strains isolated from the feces of dogs with and without diarrhea. MATERIALS AND METHODS Stool samplesStool samples were collected from 154 dogs, of which 92 were diarrheic and 62 were without diarrhea. Samples from diarrheic dogs were obtained directly from the rectum, at the Veterinary Hospital of Universidade Federal de Minas Gerais (Belo Horizonte city), upon admission. Collections were performed from dogs that were undergoing a consultation for the occurrence of diarrhea. With owner's consent, fecal samples from non-diarrheic animals were obtained from the dogs of students attending the Universidade Federal de Minas Gerais. For non-diarrheic dogs, fecal samples were either taken directly from the rectum or after spontaneous ...

show abstract

“…Costa et al [5] reported a frequency of 10.5% of MDR E. coli isolates among healthy pets what is quite similar to our results in Brazil. In recent years cases of shared fecal E. coli isolates between dogs and their owners have been reported [12]. Thus, the significance of feline feces as a reservoir of antimicrobial-resistant E. coli for their owners should be carefully examined and effective monitoring of resistant organism and tracking the movement of the resistance genes is essential if resistance and resistant organisms want to be controlled.…”

Section: Resultsmentioning

confidence: 99%

“…Due to the great number of different clones of E. coli that can be found among the commensal strains in the micro biota of the intestinal tract, at least 5 isolates from each cat were selected to be tested against antimicrobial drugs [2,12]. The susceptibility to 16 antimicrobial drugs for these isolates is shown in Table 1.…”

Section: Resultsmentioning

confidence: 99%

Untitled

2017

JMEN

View full text Add to dashboard Cite

The number of pets has increased substantially in modern society and attention is increasingly devoted to pet welfare. The aim of the present study is to investigate the occurrence of antimicrobial resistance in Escherichia coli isolates present in cat intestinal micro biota. A hundred-ninety E. coli isolates from diarrheic (n=19) and healthy (n=21) cats from northwest of São Paulo State, Brazil were examined for resistance to 16 antimicrobial drugs. The predominantly observed resistance was to tetracycline (30.5%), ampicillin (20.0%) and cotrimoxazole (17.9%) among the isolates from healthy cats and to cephalothin (42.1%), tetracycline (20.0%) and ampicillin (15.8%) among the isolates from diarrheic cats. A moderate multidrug-resistance was found among 17.8% and 8.4% of the isolates from healthy and diarrheic cats, respectively. The E. coli isolates from healthy cats may act as a reservoir of resistance genes. To support the development of antimicrobial usage policies, regular updates on the status of resistance to antimicrobial agents used in veterinary medicine are needed.

show abstract

Antimicrobial resistance, virulence profiles, and phylogenetic groups of fecal Escherichia coli isolates: A comparative analysis between dogs and their owners in Japan

Cited by 38 publications

References 26 publications

Transfer of Multidrug-Resistant Bacteria Between Intermingled Ecological Niches: The Interface Between Humans, Animals and the Environment

Transfer of Multidrug-Resistant Bacteria Between Intermingled Ecological Niches: The Interface Between Humans, Animals and the Environment

Detection of virulence genes and the phylogenetic groups of Escherichia coli isolated from dogs in Brazil

Untitled

Contact Info

Product

Resources

About