Antimicrobial Resistance Patterns in Methicillin-Resistant &lt;b&gt;&lt;i&gt;Staphylococcus aureus&lt;/i&gt;&lt;/b&gt; from Patients Hospitalized during 2015–2017 in Hospitals in Poland

Kot, Barbara; Wierzchowska, Kamila; Piechota, Małgorzata; Grużewska, Agata

doi:10.1159/000501788

Cited by 69 publications

(62 citation statements)

References 21 publications

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“…Methicillin-resistant S. aureus (MRSA) are insusceptible to all beta-lactams, including antibiotics with beta-lactamase inhibitors and carbapenems. Moreover, resistance to methicillin accompanies the resistance to other groups of antibiotics (macrolides, lincosamides, aminoglycosides, fluoroquinolones, and sulfonamides), and determines multiple drug resistance [18].…”

Section: Antibacterial Activity Against Clinical Methicillin-resistanmentioning

confidence: 99%

N-(2-Arylmethylthio-4-Chloro-5-Methylbenzenesulfonyl)amide Derivatives as Potential Antimicrobial Agents—Synthesis and Biological Studies

Żołnowska

Sławiński

Garbacz

et al. 2019

IJMS

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Rising resistance of pathogenic bacteria reduces the options of treating hospital and non-hospital bacterial infections. There is a need to search for newer chemotherapies that will show antimicrobial ability against planktonic cells as well as bacterial biofilms. We have synthesized a series of N-(2-arylmethylthio-4-chloro-5-methylbenzenesulfonyl)amides, namely, molecular hybrids, which include a 2-mercaptobenzenosulfonamide fragment and either cinnamic or cyclohexylpropionic acid residues. The antimicrobial activity of compounds 8–17 was evaluated on Gram-positive, Gram-negative bacteria and fungal species. Experiments took into account investigation of antibacterial activity against planktonic cells as well as biofilms. Compounds 8–17 showed high bacteriostatic activity against staphylococci, with the most active molecules 10 and 16 presenting low MIC values of 4–8 μg/mL against reference methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-sensitive S. aureus (MSSA) strains as well as clinical isolates. Compounds 10 and 16 also showed an ability to inhibit biofilms formed by MRSA and MSSA. The potential of 10 and 16 as antibiofilm agents was supported by cytotoxicity assays that indicated no cytotoxic effect either on normal cells of human keratinocytes or on human cancer cells, including cervical, colon, and breast cancer lines.

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Section: Antibacterial Activity Against Clinical Methicillin-resistanmentioning

confidence: 99%

N-(2-Arylmethylthio-4-Chloro-5-Methylbenzenesulfonyl)amide Derivatives as Potential Antimicrobial Agents—Synthesis and Biological Studies

Żołnowska

Sławiński

Garbacz

et al. 2019

IJMS

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“…Methicillin-resistant Staphylococcus aureus (MRSA) are responsible for hard-to-treat infections among different patient populations globally, and pose a growing problem for human health. MRSA are recognized as resistant to β-lactam antibiotics, but often MRSA strains also show resistance to many commonly used antibiotic groups, such as aminoglycosides, fluoroquinolones, macrolides, tetracycline and chloramphenicol [1]. The limited treatment options of MRSA infections result in higher mortality and increased financial costs.…”

Section: Introductionmentioning

confidence: 99%

Effect of trans-Cinnamaldehyde on Methicillin-Resistant Staphylococcus aureus Biofilm Formation: Metabolic Activity Assessment and Analysis of the Biofilm-Associated Genes Expression

Kot

Sytykiewicz

Sprawka

et al. 2019

IJMS

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The effects of trans-cinnamaldehyde (TC) on transcriptional profiles of biofilm-associated genes and the metabolic activity of two methicillin-resistant Staphylococcus aureus (MRSA) strains showing a different degree of adherence to polystyrene, were evaluated. Metabolic activity of S. aureus in biofilm was significantly decreased in the presence of TC at 1/2 minimum biofilm inhibition concentration (MBIC). Expression levels of the genes encoding laminin binding protein (eno), elastin binding protein (ebps) and fibrinogen binding protein (fib) in the presence of TC at 1/2 MBIC were lower than in untreated biofilm in both the weakly and strongly adhering strain. The highest decrease of expression level was observed in case of fib in the strongly adhering strain, in which the amount of fib transcript was 10-fold lower compared to biofilm without TC. In the presence of TC at 1/2 MBIC after 3, 6, 8 and 12 h, the expression level of icaA and icaD, that are involved in the biosynthesis of polysaccharide intercellular adhesin, was above half lower in the weakly adhering strain compared to biofilm without TC. In the strongly adhering strain the highest decrease in expression of these genes was observed after 3 and 6 h. This study showed that TC is a promising anti-biofilm agent for use in MRSA biofilm-related infections.

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“…Concordance or discordance between the results of the two were calculated according to the following FDA definitions [1]. Essential agreement or "Minor errors" [2], Categorical agreement or "No Error" [3], Major errors [4], Very major errors [33].…”

Section: Fig 2 Agarose Gel Showing Pcr Amplified Products Of Mec a mentioning

confidence: 99%

“…USA reports 35% of their hospital strains of S. aureus as methicillin resistant which were declining till 2012 but has remained almost steady in recent studies [3]. In Europe 44% of the hospital strains of S. aureus were methicillin resistant which has increased in the previous years [4].…”

Section: Introductionmentioning

confidence: 99%

Direct Detection of Methicillin Resistant Staphylococci: Comparison of Phenotypic Methods with Multiplex PCR and Direct Susceptibility Testing

Bashir

Nissar

Wani

et al. 2020

JAMB

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Bloodstream infections are augean diseases characterized by a high morbidity and mortality, related with the lag in administration of the first adequate anti-infectious agent. Clinical and epidemiological data guide the clinicians towards empirical anti-infectious treatments whose effectualness remains questionable especially in the present day milieu of multidrug-resistant organisms. Early microbiological evidence of the causative agent is hierophantic of antimicrobial stewardship. We evaluated three rapid methods for the direct identification of S. aureus from 720 positive blood cultures in comparison to Vitek 2 automated microbial identification system. Early distinction S. aureus from CoNS was attempted with Direct Tube Coagulase. Multiplex PCR assay was used to separate MRSA from MSSA by the presence of mecA gene. Direct antibiotic susceptibility (DST) from positive blood culture bottles was performed in an endeavour to reduce time and compared to the routine Vitek 2 compact antimicrobial susceptibility. For Direct tube coagulase at 4 hrs of incubation sensitivity was 79.6% while specificity was 100% however, on overnight incubation sensitivity increased to 97.9% with 100% specificity. The positive predictive value and negative predictive values were 100% and 96.9% respectively when compared to Vitek 2 identification of staphylococcal species. By DST Categorical agreement of 95% was seen for coagulase negative staphylococci microorganism-antibiotic combinations. Categorical agreement of 89.7% was seen for S. aureus microorganism-antibiotic combinations. Direct multiplex PCR testing did not misidentify any S. aureus isolate compared to Vitek 2. In 12 methicillin resistant CoNS isolates mecA gene was not detected by PCR. In total 13(3.5%) strains of staphylococci identified by DST as methicillin resistant were not identified by PCR analysis. Each of the tests has positive qualities and all may have a place in a Gram positive cocci algorithm for testing blood cultures depending on the laboratory setting, workload volume and staffing. However, rapid detection methods are a pressing priority.

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Antimicrobial Resistance Patterns in Methicillin-Resistant <b><i>Staphylococcus aureus</i></b> from Patients Hospitalized during 2015–2017 in Hospitals in Poland

Cited by 69 publications

References 21 publications

N-(2-Arylmethylthio-4-Chloro-5-Methylbenzenesulfonyl)amide Derivatives as Potential Antimicrobial Agents—Synthesis and Biological Studies

N-(2-Arylmethylthio-4-Chloro-5-Methylbenzenesulfonyl)amide Derivatives as Potential Antimicrobial Agents—Synthesis and Biological Studies

Effect of trans-Cinnamaldehyde on Methicillin-Resistant Staphylococcus aureus Biofilm Formation: Metabolic Activity Assessment and Analysis of the Biofilm-Associated Genes Expression

Direct Detection of Methicillin Resistant Staphylococci: Comparison of Phenotypic Methods with Multiplex PCR and Direct Susceptibility Testing

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